scioCyto : Cytokine Profiling

scioCyto : Cytokine profiling

scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell cultur supernatants can be used in this robust assay. The complete analysis service comprises all steps from protein extraction from your samples to a comprehensive study report for interpretation of the data.

Features

up to 119 cytokines and chemokines analysed in a single assay
robust assay with 8 technical replicates
most antibodies available for follow-up studies
phosphorylation & ubiquitination status on request

Benefits for your research

comprehensive cytokine and chemokine signalling information
low sample volumes (10 μL plasma/serum)
sensitivity as ELISAs
analysis possible from various sample types, including plasma/serum, tissue, cells, supernatants, cerebrospinal fluid (CSF)
complete analysis service from protein extraction to bioinformatic data interpretation and a comprehensive study report

scioCyto analysis process

Applications

Plasma samples

Immune system activity
Immune response profile
Biomarkers

Tissue samples

Microenvironment analysis
3D cell culture models
Immune signalling
Inflammation status

Cell samples

Cellular signalling
Immune cell activation
Growth/stimuli profiling
Supernatant analysis

You need more coverage? Have a look at our scioCD or scioDiscover service!

scioCyto target proteins

scioCyto enables a robust profiling of up to 119 cytokines and chemokines in one single assay from minute sample amounts.

Chemokines
C-C-Motive	CCL1 , CCL2 , CCL3 , CCL4 , CCL5 , CCL7 , CCL8 , CCL11 , CCL13 , CCL14 , CCL15 , CCL16 , CCL17 , CCL18 , CCL19 , CCL20 , CCL21 , CCL22 , CCL23 , CCL24 , CCL25 , CCL26 , CCL27 , CCL28
C-X-C motif	CXCL1 , CXCL5 , CXCL6 , CXCL7 (PPBP) , CXCL8 (IL-8) , CXCL9 , CXCL10 , CXCL11 , CXCL12 (SDF-1) , CXCL13 , CXCL14 , CXCL16
C motif	XCL1
C-X3-C motif	CX3CL1

Cytokines
Interferons	IFNA1 , IFNG , IFNL1 , IFNL2 , IFNL3
Interleukins	IL-1A , IL-1B , IL-2 , IL-3 , IL-4 , IL-5 , IL-6 , IL-7 , IL-8 (CXCL8) , IL-9 , IL-10 , IL-12p70 , IL-12B , IL-13 , IL-15 , IL-16 , IL-17 , IL-17B , IL-17C , IL-17F , IL-18 , IL-19 , IL-20 , IL-22 , IL-23A , IL-25 , IL-27A , IL-28A (IFNL2) , IL-28B (IFNL3) , IL-29 (IFN-L1) , IL-31 , IL-32 , IL-33 , IL-34 , IL-36G , IL-37
TNF-Family	TNFA (TNF) , TNFB (LTA) , TNFSF4 (CD252) , TNFSF5 (CD154, CD40L) , TNFSF6 (FASLG, CD95-L) , TNFSF7 (CD70, CD27-L) , TNFSF8 (CD153, CD30-L) , TNFSF10 (TRAIL, CD253) , TNFSF11 (CD254, OPG-L) , TNFSF12 (APO3-L) , TNFSF13 (APRIL, CD256) , TNFSF13B (BAFF, CD257) , TNFSF14 (CD258, HVEM-L) , TNFSF18 (AITRL)

Cytokine / growth factor activity
Colony-stimulating factors (CSF)	M-CSF , GM-CSF , G-CSF
TGF-Family	TGFB1 , TGFB2 , TGFB3
VEGF-Family	VEGF165/VEGF121 , VEGFA , VEGFC
Others	CNTF , CRLF2 , CYTL1, FGF2 , FLT3L , GDF2 , GDF15 , GRN, HGF , HMGB1 , IGF1 , INHBA , LIF , MIF , NGF-beta , ONCM , OSTP , PLF4 , TSLP

Need more coverage? Have a look at our scioCD or scioDiscover service!