scioCyto : Cytokine profiling

scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell cultur supernatants can be used in this robust assay. The complete analysis service comprises all steps from protein extraction from your samples to a comprehensive study report for interpretation of the data.

Features

  • up to 119 cytokines and chemokines analysed in a single assay
  • robust assay with 8 technical replicates
  • most antibodies available for follow-up studies
  • phosphorylation & ubiquitination status on request
 		  

Benefits for your research

  • comprehensive cytokine and chemokine signalling information
  • low sample volumes (10 μL plasma/serum)
  • sensitivity as ELISAs
  • analysis possible from various sample types, including plasma/serum, tissue, cells, supernatants, cerebrospinal fluid (CSF)
  • complete analysis service from protein extraction to bioinformatic data interpretation and a comprehensive study report

 

scioCyto analysis process

 

Applications

    

Plasma samples 

  • Immune system activity
  • Immune response profile
  • Biomarkers

Tissue samples

  • Microenvironment analysis
  • 3D cell culture models
  • Immune signalling
  • Inflammation status

Cell samples

  • Cellular signalling
  • Immune cell activation
  • Growth/stimuli profiling
  • Supernatant analysis

 

 You need more coverage? Have a look at our scioCD or scioDiscover service!

Contact us  

 

 scioCyto target proteins

 

scioCyto enables a robust profiling of up to 119 cytokines and chemokines in one single assay from minute sample amounts.
scioCyto target cytokines for profiling

 

Chemokines
C-C-Motive


 CCL1 ,   CCL2 ,   CCL3 ,   CCL4 ,   CCL5 ,   CCL7 ,   CCL8 ,   CCL11 ,   CCL13 ,   CCL14 ,   CCL15 ,   CCL16 ,   CCL17 ,   CCL18 ,   CCL19 ,   CCL20 ,   CCL21 ,   CCL22 ,   CCL23 ,   CCL24 ,   CCL25 ,   CCL26 ,   CCL27 ,   CCL28
C-X-C motif 

 CXCL1 ,   CXCL5 ,   CXCL6 ,   CXCL7 (PPBP) ,   CXCL8 (IL-8) ,   CXCL9 ,
CXCL10 ,   CXCL11 ,   CXCL12 (SDF-1) ,   CXCL13 ,   CXCL14 ,   CXCL16 
C motif XCL1
C-X3-C motif CX3CL1
   

Cytokines
Interferons IFNA1 ,   IFNG ,   IFNL1 ,   IFNL2 ,   IFNL3

Interleukins



 IL-1A ,   IL-1B ,   IL-2 ,   IL-3 ,   IL-4 ,   IL-5 ,   IL-6 ,   IL-7 ,   IL-8 (CXCL8) ,   IL-9 ,
IL-10 ,   IL-12p70 ,   IL-12B ,   IL-13 ,   IL-15 ,   IL-16 ,   IL-17 ,   IL-17B ,   IL-17C ,   IL-17F ,   IL-18 ,   IL-19 ,
IL-20 ,   IL-22 ,   IL-23A ,   IL-25 ,   IL-27A ,   IL-28A (IFNL2) ,   IL-28B (IFNL3) ,   IL-29 (IFN-L1) ,
IL-31 ,   IL-32 ,   IL-33 ,   IL-34 ,   IL-36G ,   IL-37
TNF-Family





 TNFA (TNF) ,   TNFB (LTA) ,
TNFSF4 (CD252) ,   TNFSF5 (CD154, CD40L) ,  
TNFSF6 (FASLG, CD95-L) ,   TNFSF7 (CD70, CD27-L) ,   TNFSF8 (CD153, CD30-L) ,
TNFSF10 (TRAIL, CD253) ,   TNFSF11 (CD254, OPG-L) ,   TNFSF12 (APO3-L) ,
TNFSF13 (APRIL, CD256) ,   TNFSF13B (BAFF, CD257) ,
TNFSF14 (CD258, HVEM-L) ,   TNFSF18 (AITRL) 
   

Cytokine / growth factor activity
Colony-stimulating factors (CSF) M-CSF ,   GM-CSF ,   G-CSF
TGF-Family TGFB1 ,   TGFB2 ,   TGFB3
VEGF-Family VEGF165/VEGF121 ,   VEGFA ,   VEGFC
Others










 CNTF ,   CRLF2 ,   CYTL1,
FGF2 ,   FLT3L ,
GDF2 ,   GDF15 ,   GRN,
HGF ,   HMGB1 ,
IGF1 ,   INHBA ,
LIF ,
MIF ,
NGF-beta ,
ONCM ,   OSTP ,
PLF4 ,
TSLP

 

Contact us  

 Need more coverage? Have a look at our scioCD or scioDiscover service!

News

Article | Transpulmonary Pressure-Guided PEEP Improves Lung Protection

| October 2025 | In a porcine lung injury model, transpulmonary pressure–guided PEEP titration improved mechanics and reduced tidal stress. Sciomics supported the study by analysing cytokine, chemokine, and surface marker expression levels in plasma samples using the scioCD Precision Proteomics platform.

Mutschler et al., Optimizing Positive End-Expiratory Pressure in Asymmetric Acute Lung Injury in a Porcine Model: The Role of Transpulmonary Pressure. Int. J. Mol. Sci. 2025, 26, 9985  
Article | Long-Term Biobanked Dental Pulp Stem Cells Retain Angiogenic Potential

| September 2025 | Biobanked dental pulp stem cells preserved for years retained their angiogenic capacity and stem cell phenotype. Sciomics supported the study by analysing cell differentiation marker profiles from dental pulp stem cells using the scioCD Precision Proteomics platform.

Yamada et al., Long-Term Biobanked Dental Pulp Stem Cells Retain Angiogenic Potential for Vascularised Tissue Engineering—Laboratory Investigation. Int. Endod. J. (2025, online ahead of print)
Article | Fibroblast–monocyte signalling via TLR4 drives TNBC metastasis

| September 2025 | A TLR4-dependent axis between fibroblasts and monocytes in tumor-draining lymph nodes drives metastasis in triple-negative breast cancer. Sciomics provided protein profiling of lymph node and tumor samples to map surface- and immune-related protein changes using the scioCD protein profiling platform.

Mattavelli et al., A TLR4-dependent fibroblast-monocyte axis in tumor-draining lymph nodes contributes to metastasis in triple-negative breast cancer. Immunity. 2025 Sep 15 (online ahead of print)  
Article | Extracorporeal photopheresis alters cytokine profile in CTCL

| September 2025 | ECP rapidly modulates cytokine levels and immune cell composition in CTCL patients, revealing strong systemic immune effects. Sciomics‘ scioCD platform was used to profile plasma proteins and identify cytokine and surface marker changes in CTCL patients.

Melchers et al., Extracorporeal photopheresis therapy rapidly changes the cytokine profile and tumor microenvironment in cutaneous T cell lymphoma. Front Immunol. 2025;16:1669015  
Article | Lactate-driven immune hotspots resolved via SLC5A12 blockade

| August 2025 | Lactate signaling induces cytokine production and T/B cell clustering in Sjögren’s disease, and SLC5A12 blockade reverses these effects. Sciomics contributed to this study by comparing protein abundance and acetylation levels of murine cells using the scioDiscover protein profiling platform.

Certo, M, et al.. Lactate signalling leads to aggregation of immune-inflammatory hotspots and SLC5A12 blockade promotes their resolution. Nat Metab. 2025;7(8):1663-1680.  

Meet us

life—the biomedical convention (Feb 4, 2026)

Meet us at life—the biomedical convention in Heidelberg—the bioRN annual conference.

Testimonials

PD Dr. med. Mascha O. Fiedler-Kalenka and Dr. med. Benjamin Seybold

Heidelberg University Hospital, Department of Anesthesiology, Heidelberg, GermanyGerman Center for Lung Research (DZL), Translational Lung Research Center Heidelberg (TLRC), Heidelberg, Germany

"From the very beginning, working with the friendly and highly competent team at Sciomics was both professional and refreshingly straightforward. We particularly valued their openness to our specific research questions and the expert support they provided in planning and interpreting our exploratory cytokine analysis in a preclinical lung injury model. Communication was always on equal footing, enriched by Sciomics’ deep expertise, which also sparked new ideas for future projects."

Product: scioCD

Tara Sigdel, PhD

Associate Professor, Department of Surgery, University of California San Francisco

"We collaborated with Sciomics GmbH in Germany to analyze proteomics data from blood samples of kidney transplant recipients and liver perfusates during normothermic machine perfusion. Throughout the process, Sciomics provided exceptional support, ensuring timely data delivery and valuable assistance with data analysis. Their expertise and commitment have been instrumental in advancing our research, and we are currently using their data in multiple manuscript submissions. I highly recommend Sciomics for their reliable service and expertise in proteomics."

Product: scioDiscover

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