scioCD - Cell Surface Marker and Cytokine Profiling

Fluorescence image of a scioCD antibody microarray for the profiling of cytokines and cell surface markers

Immune response profiling

Cellular composition and signalling

CD-Marker Expression Profiles

 

 

 

Our scioCD profiling service covers 349 different proteins:

Protein interaction network of 119 cytokines / chemokines and 140 cell surface markers screened in parallel by our scioCD protein profiling service

  • up to 141 unique CD-marker molecules
  • up to 119 Cytokines/Chemokines
  • other relevant proteins such as various HLAs and p53


All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of samples and signalling in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently.

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

 

 

      Analysis
Service       Sample
Types       Contact
us

 

  

Applications

  • profiling of cell differentiation status
  • stem cell differentiation 
  • investigate soluble CD-molecules for e.g. allograft rejection
  • inflammatory response profiling
  • immuno-oncology profiling of check-point inhibitor targets
  • characterisation of tissue-isolated primary cells
  • immune-cell surface marker profiling on
    • various B-cells 
    • stimulated T-cells
    • patient derived T-cells
    • patient derived dendritic cells
  • oncology profiling for new biomarkers e.g. Leukemia
  • tumor micro-environment profiling
  • profile response to stimuli of cell lines
  • evaluate protein expression changes in silencing/overexpression  experiments
  

Advantages

  • high content: up to 349 proteins are analysed
  • broad coverage and parallel analysis of
    • CD molecules 
    • cytokines and chemokines
    • other relevant molecules (HLAs, p53)
  • minimal sample requirements
  • native matrixes ( non-fractionated / non-depleted )
  • sensitivity as ELISA or better
  • fully immuno-based assay
  • reliable dual-colour study design
  • high reproducibility, CV < 10%
  • most proteins are covered redundantly by two or more antibodies
  • each antibody is presented in four replicates

The assay

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

  • plasma  or serum
  • fresh frozen tissue samples
  • cellular samples
  • cell culture samples
  • cerebrospinal fluid (CSF)
  • additional sample types on request

  

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report


 

Latest Scientific Publications using scioCD

Article | Transpulmonary Pressure-Guided PEEP Improves Lung Protection

| October 2025 | In a porcine lung injury model, transpulmonary pressure–guided PEEP titration improved mechanics and reduced tidal stress. Sciomics supported the study by analysing cytokine, chemokine, and surface marker expression levels in plasma samples using the scioCD Precision Proteomics platform.

Mutschler et al., Optimizing Positive End-Expiratory Pressure in Asymmetric Acute Lung Injury in a Porcine Model: The Role of Transpulmonary Pressure. Int. J. Mol. Sci. 2025, 26, 9985  

Article | Long-Term Biobanked Dental Pulp Stem Cells Retain Angiogenic Potential

| September 2025 | Biobanked dental pulp stem cells preserved for years retained their angiogenic capacity and stem cell phenotype. Sciomics supported the study by analysing cell differentiation marker profiles from dental pulp stem cells using the scioCD Precision Proteomics platform.

Yamada et al., Long-Term Biobanked Dental Pulp Stem Cells Retain Angiogenic Potential for Vascularised Tissue Engineering—Laboratory Investigation. Int. Endod. J. (2025, online ahead of print)

Article | Fibroblast–monocyte signalling via TLR4 drives TNBC metastasis

| September 2025 | A TLR4-dependent axis between fibroblasts and monocytes in tumor-draining lymph nodes drives metastasis in triple-negative breast cancer. Sciomics provided protein profiling of lymph node and tumor samples to map surface- and immune-related protein changes using the scioCD protein profiling platform.

Mattavelli et al., A TLR4-dependent fibroblast-monocyte axis in tumor-draining lymph nodes contributes to metastasis in triple-negative breast cancer. Immunity. 2025 Sep 15 (online ahead of print)  

Article | Extracorporeal photopheresis alters cytokine profile in CTCL

| September 2025 | ECP rapidly modulates cytokine levels and immune cell composition in CTCL patients, revealing strong systemic immune effects. Sciomics‘ scioCD platform was used to profile plasma proteins and identify cytokine and surface marker changes in CTCL patients.

Melchers et al., Extracorporeal photopheresis therapy rapidly changes the cytokine profile and tumor microenvironment in cutaneous T cell lymphoma. Front Immunol. 2025;16:1669015  

Article | Altered Serum Proteins indicate Inflammation, Fibrogenesis and Angiogenesis in Fontan Patients

| September 2024 | Four identified serum proteins may advance diagnostic and therapeutic approaches by complementing traditional diagnostic laboratory variables. Sciomics contributed to this study by a scioCD serum analysis targeting different CD surface markers, cytokines and chemokines.

Michel M, Renaud D, Schmidt R, et al. Altered Serum Proteins Suggest Inflammation, Fibrogenesis and Angiogenesis in Adult Patients with a Fontan Circulation. International Journal of Molecular Sciences. 2024; 25(10):5416. 
 

Testimonials

PD Dr. med. Mascha O. Fiedler-Kalenka and Dr. med. Benjamin Seybold

Heidelberg University Hospital, Department of Anesthesiology, Heidelberg, GermanyGerman Center for Lung Research (DZL), Translational Lung Research Center Heidelberg (TLRC), Heidelberg, Germany

"From the very beginning, working with the friendly and highly competent team at Sciomics was both professional and refreshingly straightforward. We particularly valued their openness to our specific research questions and the expert support they provided in planning and interpreting our exploratory cytokine analysis in a preclinical lung injury model. Communication was always on equal footing, enriched by Sciomics’ deep expertise, which also sparked new ideas for future projects."

Product: scioCD

Tara Sigdel, PhD

Associate Professor, Department of Surgery, University of California San Francisco

"We collaborated with Sciomics GmbH in Germany to analyze proteomics data from blood samples of kidney transplant recipients and liver perfusates during normothermic machine perfusion. Throughout the process, Sciomics provided exceptional support, ensuring timely data delivery and valuable assistance with data analysis. Their expertise and commitment have been instrumental in advancing our research, and we are currently using their data in multiple manuscript submissions. I highly recommend Sciomics for their reliable service and expertise in proteomics."

Product: scioDiscover

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