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scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation.

Features

  • 1,300 highly relevant proteins are profiled in a single assay
  • Phosphorylation status combined with protein expression levels
  • Tyrosine, serine and threonine phosphorylation
  • Analysis of individual phosphorylation types available
  • "Sample-to-result" service
  • Comprehenisive data analysis and presentation
  • Individualised study report
Protein abundance levels and phosphorylation status for one individual protein out of 900 proteins tested

Advantages

  • High-content screening of proteins and phosphorylation is time and ressource efficient
  • Direct comparability through protein expression and phosphorylation level analysis in a single assay
  • Complete analysis from minimal sample volume
  • Coverage of important
    • Signalling pathways
    • Transcription factors
    • Receptor molecules
    • Kinases
  • Individual data analysis options available
    • Protein interaction network analysis
    • Biological and pathway classification 
    • Lists with differential proteins and phosphorylation levels
  • Variety of sample formats possible without the potential bias of a sample fractionation or a sample depletion
  • Results presented in a publication-ready format

Applications

  • T cell activation
  • Tissue treatment response
  • Cell cycle regulation
  • Oxidative stress response
  • Cell adhesion and motility
  • Phosphorylation profiling of various signalling pathways:
    • FAK phosphorylation
    • PI3K-AKT pathway
    • p53 pathway regulation
    • Alzheimer pathway regulation

 

Case study   Target proteins   Contact us

 

Case study

Eight treated cell line samples were compared with 8 control samples. Several proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). While the left plot provides an overview on expression / phosphorylation differences for all tested proteins in the two sample groups, individual levels are depicted in the plots on the right. The sample status is indicated as C = control or T = treated.

 

Reference customers

Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany

Dr. Caroline Pabst. University Hospital Halle, Germany

 

More information

Download our scioPhospho or scioDiscover service brochure.

Information on protein and pathway content / coverage.

Get in touch to answer any remaining questions.

Ask for a quotation to get your study started.

 

Which sample types can be analysed?

With the scioPhospho service a variety of samples can be analysed:

  • fresh frozen tissue samples
  • cellular content
  • cerebrospinal fluid
  • plasma / serum
  • additional sample types on request

 

Sample-to-result service

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • statistical testing for differentially phosphorylated proteins
  • comprehensive study report

 

Our service portfolio

scioPhospho: protein profiling and phosphorylation status

scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation. Features 1,300 highly relevant proteins are profiled in a single assay Phosphorylation status combined with protein expression levels…
Read more
scioDiscover - Protein Profiling on expression level

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scioCD - Cell surface marker and Cytokine profiling

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COVID-19 related activities and services

covid 19 portfolio web

News

New article | Prevention of Cyclosp.-A mediated anemia and peritubular loss

| June 2022 | In a study by Robert Labes et al. insights in prevention of cyclosporine mediated anemia and peritubular capillary loss were identified.

Labes, R. et al. Daprodust. prevents cyclosp.-A mediated anemia and peritubular capillary loss. Kidney International 2022 in press.
New article | The Lifestyle Modifications Affect the Endometrial Proteome

| June 2022 | In a study by D. Abdulkhalikova et al. Endometrial Proteome changes werde identified impacted by Lifestyle modification in woman with Polycystic Ovarian Syndrome and Obesity.

Abdulkhalikova, DR. et al. The Lifestyle Modifications and Endometrial Proteome Changes of Women With Polycystic Ovary Syndrome and Obesity. Front. Endocrinol. 2022 in press.
New article | Cycle-dependent biomarkers in endometriosis

| May 2022 | In a study by Maja Pušić et al. Cycle-dependent plasma protein biomarkers for peritoneal endometriosis were identified by our scioDiscover platform.

Pušić, M. et al. Antibody Arrays Identified Cycle-Dependent Plasma Biomarker Candidates of Peritoneal Endometriosis. J. Pers. Med. 202212, 852.
New article | Co-Expression of microRNAs and Proteins in Alzheimer’s Disease

| Jan 2022 | In a study by Valentina di Pietro at Birmingham University our scioDoiscover platform was used to analyse the co-expression of proteins and microRNAs in the brains of Alzheimer's disease patients.

Watson, C. N. et al. Co-Expression Analysis of microRNAs and Proteins in Brain of Alzheimer’s Disease Patients. Cells 11, 163 (2022).
New article | ETV6 levels predict outcome in DLBCL

| Jan 2022 | Erich Piovan and collegues at University of Padua collaborated with Sciomics to study specific biomarkers in FFPE tissue samples of DLBCL patients by custom antibody microarrays.

Marino, D. et al. High ETV6 Levels Support Aggressive B Lymphoma Cell Survival and Predict Poor Outcome in Diffuse Large B-Cell Lymphoma Patients. Cancers 14, (2022).

Testimonials

Dr. Bettina Grötsch

Department of Medicine 3 - Rheumatology and Immunology, University Clinics Erlangen, Erlangen, Germany

"Our lab is working with human osteoclast that were isolated and differentiated from human peripheral blood. Therefore, we were quite limited in cell numbers and protein content for our planned Phosphoprotein analyses. Nonetheless, Sciomics and especially Camille Lowy helped us a lot to test and improve our protein isolation techniques to achieve some interesting phosphoproteome data in human osteoclasts. We were really impressed by the detailed data report and their willingness to discuss and reanalyze the data repeatedly until we got the best results out of our measurements. Even several month after their first report, we could ask for help and suggestions with an immediate reply.  The phosphoproteome analyses completed our preliminary project data and are now published in Frontiers Immunology.  Altogether, we were very happy with the service of Sciomics and special thanks to Camille Lowy and Christoph Schröder who guided us through the whole process."

Product: scioPhospho
Publication: Grötsch B. et al. 2022 Front. Immunol. 13:958974 

Alenka Djarmila Behsen, PhD candidate

Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, Trondheim, Norway

"The process of working with Ronny and his colleagues at Sciomics has been very easy and efficient. Response time is very quick and they are always available for meetings when you have more questions. Would definitely recommend working with Sciomics."

Product: scioCD

 

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