scioDiscover - Protein Expression Analysis

Protein expression profiling with protein arrays

The scioDiscover microarray platform enables high-content, immuno-based protein profiling of diverse biological samples. It provides a comprehensive overview of proteomic changes by analyzing more than 1,400 proteins in parallel. It covers key pathways in diseases such as cancer, neurological disorders and organ failure. The technology has been successfully applied in numerous biomedical studies and in the discovery of new biomarker signatures. scioDiscover enables rapid and targeted research while providing a broad overview of potential changes at the protein level in your samples.


Features and Benefits

  • Covers major signaling pathways, secreted proteins, cell surface and inflammatory markers
  • more than 1,400 proteins are profiled
  • Wide range of indications covered, including oncology and organ failure
  • Robust dual-colour study design
  • Complete data analysis and study report
  • Studies in human, murine and rat samples possible*
  • Saves time, money and valuable sample




Sample-to-Result ServiceAntibody microarray workflow

As part of our analysis service, we will not only perform the microarray experiments, but also support you with a proposal for an appropriate microarray study design and sample selection in order to optimally address your scientific question. You will receive a customized study report including statistical analysis within 3-4 weeks after receipt of your samples.

Our analysis service includes:

  • Definition of a suitable study design
  • Sample preparation
  • Protein extraction
  • Protein concentration measurements
  • Protein quality control
  • Sample labeling
  • Sample purification
  • Incubation of the samples on antibody microarrays
  • Microarray scanning
  • Raw data acquisition
  • Data normalization
  • Data analysis including cluster analysis
  • Statistical testing of differentially abundant proteins
  • Comprehensive study report


Analysis and Profiling of

  • Protein expression levels
  • Signaling pathways
  • Immune system activation markers
  • Transcription factors
  • Cancer pathway screening
  • Cell surface markers
  • Apoptotic processes/ apoptosis pathway
  • Disease mechanisms
  • Pathway activation in stem cells 
  • Organ damage/failure models 
  • Response of cells and tissue models to oxidative stress
  • Proteomic profiling for different cell culture conditions
  • Molecular initiate event analysis
  • Mode-of-action analysis of compounds
  • Prediction markers for toxicological effects
  • CD cell surface molecule expression in immune cells  



  • High-content protein profiling of more than 1,400 proteins 
  • Broad coverage of:
    • Signaling pathway proteins
    • Transcription factors
    • Apoptosis markers
    • Oxidative stress markers
    • Cell surface markers
    • Cytokines & chemokines
  • Minimum sample requirements
  • Native matrices (non-fractionated / non-depleted)
  • Sensitivity equal to or better than ELISA
  • Fully immuno-based assay
  • Reliable dual-color study design
  • Technical replication (4 replicates for each antibody)
  • High reproducibility (CV < 10%)

Our Team:

  • Over a decade of experience in biomedical antibody array studies
  • Reliable support from planning to the final analysis
  • Save time, money and precious sample



Which sample types can be analyzed ?

A wide range of samples can be analyzed with scioDiscover

  • Plasma or serum
  • Fresh-frozen tissue samples
  • Cellular samples
  • Cell culture supernatants
  • Cerebrospinal fluid (CSF)
  • Other sample types upon request
  • Studies can be performed on human, mouse or rat samples*

A minimal amount of sample is required, for example**

  • Plasma sample: 5µl
  • Tissue: 1mg (fresh frozen)
  • Cells: 100,000
  • CSF: 5µl


Reference Customers:

  • Prof. Dr. Faikah Güler, Molecular mechanisms of transplant dysfunction, Medical School Hanover, Hanover, Germany
  • Julian Kamhieh-Milz, Research Center ImmunoSciences, Charité, Berlin, Germany
  • Prof. Jose L. Peiró, Cincinnati Fetal Center Director, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, USA

Case studies:

Bladder Cancer

Acute Kidney Injury


 * Antibody array is designed to target human proteins. Due to a high sequence homology of the vast majority of proteins, it has often been successfully used to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].
**Minimum quantity. These values may vary depending on the sample type, study design, storage conditions. and additional parameters.

Our service portfolio

scioPhospho: protein profiling and phosphorylation status

scioPhospho: protein profiling and phosphorylation status

Our scioPhospho analysis combines protein expression profiling using scioDiscover with analysis of the corresponding protein's phosphorylation status. More than 1,400 proteins are analyzed in parallel, covering key pathways in diseases such as cancer, neurological disorders and organ failure.  This combination provides a comprehensive overview of signaling events and pathway activity…
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scioUbi: protein profiling and ubiquitination status

scioUbi: protein profiling and ubiquitination status

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse more than 1,400 proteins in a single assay.   Features Screen…
scioDiscover - Protein Profiling on the Expression Level

scioDiscover - Protein Profiling on the Expression Level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…
scioCD - Cell Surface Marker and Cytokine Profiling

scioCD - Cell Surface Marker and Cytokine Profiling

Characterize Your Cell Type! Cell Composition Analysis in Tissue Samples CD-Marker Expression Profiles Increased Throughput at High Sensitivity Levels High Reproducibility       Our scioCD profiling service covers 349 different…
scioCyto : Cytokine Profiling

scioCyto : Cytokine Profiling

scioCyto : Cytokine profiling scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell…

COVID-19 related activities and services

covid 19 portfolio web


New Article | Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia

| September 2023 | New Article: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia. Sciomics supported this study by scioCyto serum analysis in a mouse cancer model.

Taylor, J., Uhl, L., Moll, I. et al. Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia. Nat Cancer 4, 1544–1560 (2023)
New Article | Rapid Diagnostic Platform for Personalized Vitamin B6 Detection in Erythrocytes

| July 2023 | New Article: Fiedler et al. developed a novel methodology for vitamin B6 diagnostics. We supported the project by the production of custom antibody microarray targeting signature human PLP-DE and testing respective samples.

New Article | Microbiota Influences Chemotherapy in Pancreatic Cancer

| February 2023 | New Article: In the study Tintelnot et al. identified a microbiota-derived metabolite that has clinical implications in the treatment of pancreatic cancer. Sciomics supported the authors by a protein profiling study of tumours derived from a mouse model upon treatment with 3-IAA and Firinox.

Tintelnot et al. Microbiota-derived 3-IAA influences chemotherapy efficacy in pancreatic cancer. Nature 615, 168-174 (2023)
New Article | Predicting a severe Covid-19 disease

| April 2023 | New Article: In a protein biomarker development project initiated by Sciomics, we have identified protein biomarker panels predicting development of a severe Covid.19  disease.

Hufnagel K., Fathi A. et al. Discovery and systematic assessment of early biomarkers that predict progression to severe COVID-19 disease. Communications Medicine 3,51 (2023)
New Sciomics Proteomics Research Blog has Launched!

Sciomics Protomics Research Blog


At Sciomics, we are dedicated to advancing healthcare through proteomics research. We are collaborating with research groups and big pharma, alongside our in-house biomarker research initiatives. To make this research more accessible to you, we are launching the new Sciomics science blog!

The first study we will feature was published this year in a journal in the Nature publishing group. It's a topic that affects us all. Any guesses? Stay tuned!




Dr. Matthew Wright

Chief Scientific Officer, Kinarus Therapeutics AG, Basel, Switzerland

"We are a small biotech company without our own wet labs. Sciomics recommended a UK CRO that performed cell culture experiments for our studies. Sciomics seamlessly coordinated the transfer of the biologic materials to their facility and performed the scioCD panel to characterize cell surface markers and cytokines in cell lines treated with our small molecule drug candidates. The Sciomics data were instrumental in supporting our IP protection strategy. Their advice was very helpful and they worked with us to extract the most out of the data. We are very satisfied and highly recommend the Sciomics platform and the team."

Product: scioCD

Kyriaki Barmpa

PhD Candidate, Developmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine, University of Luxembourg

"By collaborating with Sciomics, we conducted a detailed analysis of the proteome of striatum organoids, uncovering significant differences that further assisted our research. Their communication was highly efficient, and the results were delivered promptly."

Product: scioPhospho