@

scioDiscover - Protein Expression Analysis

Protein expression profiling with protein arrays

Our scioDiscover service enables high-content, immuno-based protein profiling of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1,300 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples.

 

Features

  • Major pathways, secreted proteins, cell surface and inflammatory markers are covered
  • 1,300 proteins are profiled
  • Broad spectrum of indications covered such as oncology and organ failure
  • Robust dual-colour study design
  • Including full data analysis and study report
  • Studies possible in human, murine and rat samples*

 

 Array
 content
    Protein
 profiling
    Biomarker
 Discovery
     Target
 Discovery
     Contact
 us

 

 

Applications

  • Protein expression level analysis
  • Signalling Pathway Profiling
  • Immunsystem activation marker profiling
  • Transcription factor analysis 
  • Cancer pathway screening
  • Cell surface marker profiling
  • Profiling of apoptotic processes/ apoptosis pathway analysis 

 

  • Disease mechanism profiling
  • Pathway activation in stem cells 
  • Analysis of organ damage/failure models 
  • Response of cells and tissue models to oxidative stress analysis
  • Profiling proteomic changes for various cell culture conditions

 

  • Molecular initiate event analysis
  • Compound mode-of-action analysis
  • Prediction markers for toxicological effects 

 

 

  • CD cell surface molecule expression in immune cells  

 

 

 

 

Advantages

  • high-content protein profiling of 1,300 proteins 
  • broad coverage of:
    • signalling pathway proteins
    • transcription factors
    • apoptosis marker
    • markers for oxidative stress
    • cell surface markers
    • cytokines & chemokines

 

  • minimal sample requirements
  • native matrixes (non-fractionated / non-depleted)
  • sensitivity as ELISA or better

 

  • fully immuno-based assay

 

  • reliable dual-colour study design
  • technical replication ( 4 replicates for each antibody )
  • high reproducibility ( CV < 10% )

 

  • experienced partners with more than ten years of experience in conducting biomedical antibody array studies

 

 

Save time, costs and valuable sample

 

 

 

Reference Customers:

  • Prof. Dr. Faikah Güler, Molecular mechanisms of transplant dysfunction, Medical School Hanover, Hanover, Germany
  • Julian Kamhieh-Milz, Research Center ImmunoSciences, Charité, Berlin, Germany
  • Prof. Jose L. Peiró, Cincinnati Fetal Center Director, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, USA
 

Case studies:

Bladder Cancer

Acute Kidney Injury


 

Which sample types can be analysed ?

With the scioDiscover service a variety of samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request
  • studies possible in samples from human, mouse or rat*

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

Sample-to-result serviceAntibody microarray workflow

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

 

Our service portfolio

scioPhospho: protein profiling and phosphorylation status

scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation. Features 1,300 highly relevant proteins are profiled in a single assay Phosphorylation status combined with protein expression levels…
Read more
scioDiscover - Protein Profiling on the Expression Level

scioDiscover - Protein Profiling on the Expression Level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…
scioUbi

scioUbi

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse up to 1,000 proteins in a single assay.   Features Screen…
scioCD - Cell Surface Marker and Cytokine Profiling

scioCD - Cell Surface Marker and Cytokine Profiling

Characterize Your Cell Type! Cell Composition Analysis in Tissue Samples CD-Marker Expression Profiles Increased Throughput at High Sensitivity Levels High Reproducibility       Our scioCD profiling service covers 260…
scioCyto : Cytokine Profiling

scioCyto : Cytokine Profiling

scioCyto : Cytokine profiling scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or…

COVID-19 related activities and services

covid 19 portfolio web

News

New Article | Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia

| September 2023 | New Article: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia. Sciomics supported this study by scioCyto serum analysis in a mouse cancer model.

Taylor, J., Uhl, L., Moll, I. et al. Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia. Nat Cancer 4, 1544–1560 (2023)
New Article | Rapid Diagnostic Platform for Personalized Vitamin B6 Detection in Erythrocytes

| July 2023 | New Article: Fiedler et al. developed a novel methodology for vitamin B6 diagnostics. We supported the project by the production of custom antibody microarray targeting signature human PLP-DE and testing respective samples.

New Article | Microbiota Influences Chemotherapy in Pancreatic Cancer

| February 2023 | New Article: In the study Tintelnot et al. identified a microbiota-derived metabolite that has clinical implications in the treatment of pancreatic cancer. Sciomics supported the authors by a protein profiling study of tumours derived from a mouse model upon treatment with 3-IAA and Firinox.

Tintelnot et al. Microbiota-derived 3-IAA influences chemotherapy efficacy in pancreatic cancer. Nature 615, 168-174 (2023)
New Article | Predicting a severe Covid-19 disease

| April 2023 | New Article: In a protein biomarker development project initiated by Sciomics, we have identified protein biomarker panels predicting development of a severe Covid.19  disease.

Hufnagel K., Fathi A. et al. Discovery and systematic assessment of early biomarkers that predict progression to severe COVID-19 disease. Communications Medicine 3,51 (2023)
New Sciomics Proteomics Research Blog has Launched!

Sciomics Protomics Research Blog

 

At Sciomics, we are dedicated to advancing healthcare through proteomics research. We are collaborating with research groups and big pharma, alongside our in-house biomarker research initiatives. To make this research more accessible to you, we are launching the new Sciomics science blog!

The first study we will feature was published this year in a journal in the Nature publishing group. It's a topic that affects us all. Any guesses? Stay tuned!

 

 

Testimonials

Dr. Matthew Wright

Chief Scientific Officer, Kinarus Therapeutics AG, Basel, Switzerland

"We are a small biotech company without our own wet labs. Sciomics recommended a UK CRO that performed cell culture experiments for our studies. Sciomics seamlessly coordinated the transfer of the biologic materials to their facility and performed the scioCD panel to characterize cell surface markers and cytokines in cell lines treated with our small molecule drug candidates. The Sciomics data were instrumental in supporting our IP protection strategy. Their advice was very helpful and they worked with us to extract the most out of the data. We are very satisfied and highly recommend the Sciomics platform and the team."

Product: scioCD

Kyriaki Barmpa

PhD Candidate, Developmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine, University of Luxembourg

"By collaborating with Sciomics, we conducted a detailed analysis of the proteome of striatum organoids, uncovering significant differences that further assisted our research. Their communication was highly efficient, and the results were delivered promptly."

Product: scioPhospho