scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation.
Eight treated cell line samples were compared with 8 control samples. Several proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). While the left plot provides an overview on expression / phosphorylation differences for all tested proteins in the two sample groups, individual levels are depicted in the plots on the right. The sample status is indicated as C = control or T = treated.
Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany
Dr. Caroline Pabst. University Hospital Halle, Germany
Information on protein and pathway content / coverage.
With the scioPhospho service a variety of samples can be analysed:
- fresh frozen tissue samples
- cellular content
- cerebrospinal fluid
- plasma / serum
- additional sample types on request
Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.
Our analysis service includes:
- definition of an appropriate study design
- sample preparation
- protein extraction
- protein concentration measurements
- protein quality control
- sample labelling
- sample purification
- incubation of the samples on antibody microarrays
- microarray scanning
- raw data acquisition
- data normalisation
- data analysis including cluster analysis
- statistical testing for differentially abundant proteins
- statistical testing for differentially phosphorylated proteins
- comprehensive study report