Our scioPhospho analysis combines protein expression profiling using scioDiscover with analysis of the corresponding protein's phosphorylation status. More than 1,400 proteins are analyzed in parallel, covering key pathways in diseases such as cancer, neurological disorders and organ failure.  This combination provides a comprehensive overview of signaling events and pathway activity regulation in your sample. scioPhosho enables targeted, rapid, and cost-effective research.

Key Features

more than 1,400 highly relevant proteins are profiled in a single assay Combines analysis of phosphorylation status with protein expression level analysis Analysis of tyrosine, serine, and threonine phosphorylation Analysis of individual phosphorylation types is available 'Sample-to-result' service Comprehensive data analysis and reporting Customized study report

Advantages High-content screening of proteins and phosphorylation is time and resource efficient Direct comparability by analyzing the level of protein expression and the level of phosphorylation in a single assay Complete analysis from a minimal amount of sample Coverage of important Signaling pathways Transcription factors Receptor molecules Kinases Custom data analysis options available Protein interaction network analysis Biological and pathway classification  Lists of differentially expressed proteins and levels of phosphorylation Variety of sample formats possible without the potential bias of sample fractionation or sample depletion Results presented in a publication-ready format

Applications T-cell activation Tissue treatment response Cell cycle regulation Oxidative stress response Cell adhesion and motility Phosphorylation profiling of various signaling pathways FAK phosphorylation PI3K-AKT pathway p53 pathway regulation Alzheimer pathway regulation

Which Sample Types can be Analyzed?

A wide range of samples can be analyzed with scioPhospho

• Fresh-frozen tissue samples
• Cellular samples
• Cerebrospinal fluid (CSF)
• Plasma or serum
• Other sample types available upon request

A minimal amount of sample is required*, for example

• Plasma sample: 5µl
• Tissue: 1mg (fresh-frozen)
• Cells: 100,000
• CSF: 5µl

 Our Sample-to-Result Service

As part of our analysis service, we will not only perform the microarray experiments but will also support you with an appropriate microarray study design and sample selection to best address your scientific questions. After we receive your samples, you will receive a customized study report including statistical analysis within 3-4 weeks.

Our analysis service includes:

• Definining a suitable study design
• Sample preparation: Protein extraction, concentration measurements, quality control, and sample purification
• Microarray analysis
• Raw data acquisition and data normalisation
• Data analysis including cluster analysis
• Statistical testing for differentially abundant proteins
• Statistical testing for differentially phosphorylated proteins
• Comprehensive study report

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Case Study

Eight treated cell line samples were compared with eight control samples. Multiple proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). The left graph provides an overview of the expression/phosphorylation differences for all tested proteins in the two sample groups, while the right graphs show individual protein levels. The sample status is indicated as C for control or T for treated.

Reference Customers

Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany

Dr. Caroline Pabst. University Hospital Halle, Germany

More Information

Download our scioPhospho or scioDiscover service brochure.

Information on protein and pathway content / coverage.

Get in touch to answer any remaining questions.

Ask for a quotation to get your study started.

*Minimum quantity. These values may vary depending on the sample type, study design, storage conditions. and additional parameters.

Protein & ubiquitination profiling

scioUbi is a high-content protein expression and ubiquitination level analysis.
With scioUbi you can analyse more than 1,400 proteins in a single assay.

Biological processes

Cell cycle: proteins in green are covered by the analysis

Applications

Contact us

You have identified a compound with the desired effect ?

You are looking for its targets ?

Which additional pathways are affected ?

Which proteins are altered in a diseased tissue ?

Get a complete picture of protein expression in multiple pathways !

In-depth knowledge about protein expression levels is crucial for the discovery and evaluation of new drug targets. Our Scio-Discover service is fully immuno-based and covers proteins involved in key disease and signaling pathways as well as secreted proteins. Only minimal sample amounts are required for highly parallel protein analysis. There is no need for sample fractionation or depletion. Our platform is capable of processing most biological samples without extensive sample preparation, saving valuable time and resources.

Applications

• Drug target identification and verification studies
• Mechanistic studies for various indications
• Correlation of next-gen sequencing (NGS) data with protein expression levels
• Profile cytokine and inflammatory status of your samples

Key Advantages

• Low sample consumption, for example*
  • Plasma sample: 5µl
  • Tissue: 1mg (fresh-frozen)
  • Cells: 100,000
  • CSF: 5µl
• Investigation of human, murine and rat samples**
• Fast turnaround time, as little as two weeks***
• No need for laborious sample depletion or fractionation
• High sensitivity (as sensitive as or more sensitive than ELISA)
• High reproducibility (CV < 10%)
• Various sample types can be analyzed
  
  • Blood serum or plasma
  • Tissue
  • Cell culture
  • Cerebrospinal fluid (CSF)

Service Features

• Study design
• Protein extraction and purification
• Protein quantification
• Protein labelling with high-performance fluorescent dyes
• Automated array incubation and handling 
• Array scanning and data acquisition
• Data analysis according to your requirements
• Customized study report

Overview
Scio-Discover     Array
Content     Biomarker
Discovery     Contact
us

*Minimum quantity. These values may vary depending on the sample type, study design, storage conditions. and additional parameters.

_{** Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].}

_{*** Depending on study design and sample numbers.}

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower cost through a highly-parallel protein expression level analysis using our Scio-Discover Antibody Microarray Platform. Furthermore, you may link to and verify your Genomics and Transcriptomics data with protein expression levels.

Applications:

• Pre-clinical Toxicology Studies
  • rodents such as mice* and rats*
  • cell culture models
  • tissue culture models
• Drug target identification and verification
• Mechanistic studies for various indications
• Profile cytokine and inflammatory status
• Protein Biomarker identification and verification

Key advantages:

• High multiplexity (more than 1,400 proteins in parallel)
• Low sample consumption
• Investigation of human and murine samples*
• Fast turnaround time, as low as two weeks**
• No need for laborious depletion or fractionation of your samples
• High sensitivity (as sensitive as an ELISA or better)
• High reproducibility (CV < 10%)
• Various sample types can be investigated
  • Plasma or Serum
  • fresh-frozen tissue samples
  • Cell culture samples
  • Cerebrospinal fluid (CSF)

Overview
Scio-Discover     Pathway
Coverage     Biomarker
Discovery     Contact
us

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

** Depending on study design and sample numbers.