scioCyto : Cytokine profiling

scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell cultur supernatants can be used in this robust assay. The complete analysis service comprises all steps from protein extraction from your samples to a comprehensive study report for interpretation of the data.

Features

  • up to 119 cytokines and chemokines analysed in a single assay
  • robust assay with 8 technical replicates
  • most antibodies available for follow-up studies
  • phosphorylation & ubiquitination status on request
 
 

Benefits for your research

  • comprehensive cytokine and chemokine signalling information
  • low sample volumes (10 μL plasma/serum)
  • sensitivity as ELISAs
  • analysis possible from various sample types, including plasma/serum, tissue, cells, supernatants, cerebrospinal fluid (CSF)
  • complete analysis service from protein extraction to bioinformatic data interpretation and a comprehensive study report

scioCyto analysis process

 

Applications

   

Plasma samples 

  • Immune system activity
  • Immune response profile
  • Biomarkers

Tissue samples

  • Microenvironment analysis
  • 3D cell culture models
  • Immune signalling
  • Inflammation status

Cell samples

  • Cellular signalling
  • Immune cell activation
  • Growth/stimuli profiling
  • Supernatant analysis

 

 You need more coverage? Have a look at our scioCD or scioDiscover service!

Contact us  

 

 scioCyto target proteins

 

scioCyto enables a robust profiling of up to 119 cytokines and chemokines in one single assay from minute sample amounts.

scioCyto target cytokines for profiling

 

Chemokines

C-C-Motive


CCL1 ,   CCL2 ,   CCL3 ,   CCL4 ,   CCL5 ,   CCL7 ,   CCL8 ,   CCL11 ,   CCL13 ,   CCL14 ,   CCL15 ,   CCL16 ,   CCL17 ,   CCL18 ,   CCL19 ,   CCL20 ,   CCL21 ,   CCL22 ,   CCL23 ,   CCL24 ,   CCL25 ,   CCL26 ,   CCL27 ,   CCL28
C-X-C motif 

CXCL1 ,   CXCL5 ,   CXCL6 ,   CXCL7 (PPBP) ,   CXCL8 (IL-8) ,   CXCL9 ,
CXCL10 ,   CXCL11 ,   CXCL12 (SDF-1) ,   CXCL13 ,   CXCL14 ,   CXCL16 
C motif XCL1
C-X3-C motif CX3CL1
   

Cytokines

Interferons IFNA1 ,   IFNG ,   IFNL1 ,   IFNL2 ,   IFNL3

Interleukins



IL-1A ,   IL-1B ,   IL-2 ,   IL-3 ,   IL-4 ,   IL-5 ,   IL-6 ,   IL-7 ,   IL-8 (CXCL8) ,   IL-9 ,
IL-10 ,   IL-12p70 ,   IL-12B ,   IL-13 ,   IL-15 ,   IL-16 ,   IL-17 ,   IL-17B ,   IL-17C ,   IL-17F ,   IL-18 ,   IL-19 ,
IL-20 ,   IL-22 ,   IL-23A ,   IL-25 ,   IL-27A ,   IL-28A (IFNL2) ,   IL-28B (IFNL3) ,   IL-29 (IFN-L1) ,
IL-31 ,   IL-32 ,   IL-33 ,   IL-34 ,   IL-36G ,   IL-37
TNF-Family





TNFA (TNF) ,   TNFB (LTA) ,
TNFSF4 (CD252) ,   TNFSF5 (CD154, CD40L) ,  
TNFSF6 (FASLG, CD95-L) 
,   TNFSF7 (CD70, CD27-L) ,   TNFSF8 (CD153, CD30-L) ,
TNFSF10 (TRAIL, CD253) ,   TNFSF11 (CD254, OPG-L) ,   TNFSF12 (APO3-L) ,
TNFSF13 (APRIL, CD256) ,   TNFSF13B (BAFF, CD257) ,
TNFSF14 (CD258, HVEM-L) ,   TNFSF18 (AITRL) 
   

Cytokine / growth factor activity

Colony-stimulating factors (CSF) M-CSF ,   GM-CSF ,   G-CSF
TGF-Family TGFB1 ,   TGFB2 ,   TGFB3
VEGF-Family VEGF165/VEGF121 ,   VEGFA ,   VEGFC
Others










CNTF ,   CRLF2 ,   CYTL1,
FGF2 ,   FLT3L ,
GDF2 ,   GDF15 ,   GRN,
HGF ,   HMGB1 ,
IGF1 ,   INHBA ,
LIF ,
MIF ,
NGF-beta ,
ONCM ,   OSTP ,
PLF4 ,
TSLP

 

Contact us  

 Need more coverage? Have a look at our scioCD or scioDiscover service!

Latest Scientific Publications using scioCyto

Article | Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia

| September 2023 | Article: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia. Sciomics supported this study by scioCyto serum analysis in a mouse cancer model.

Taylor, J., Uhl, L., Moll, I. et al. Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia. Nat Cancer 4, 1544–1560 (2023)

Article | HAPLN1 potentiates peritoneal metastasis in pancreatic cancer

| April 2023 | New Article: Wiedmann et al. identified HAPLN1 as a prognostic marker and as a and as a driver for peritoneal metastasis in pancreatic cancer. Sciomics contributed a plasma protein profiling of a mouse model to support this exciting study.

Wiedmann L et al. HAPLN1 potentiates peritoneal metastasis in pancreatic cancer. Nature Communications 14,2354 (2023)

Testimonials

Tara Sigdel, PhD

Associate Professor, Department of Surgery, University of California San Francisco

"We collaborated with Sciomics GmbH in Germany to analyze proteomics data from blood samples of kidney transplant recipients and liver perfusates during normothermic machine perfusion. Throughout the process, Sciomics provided exceptional support, ensuring timely data delivery and valuable assistance with data analysis. Their expertise and commitment have been instrumental in advancing our research, and we are currently using their data in multiple manuscript submissions. I highly recommend Sciomics for their reliable service and expertise in proteomics."

Product: scioDiscover

Dr. Matthew Wright

Chief Scientific Officer, Kinarus Therapeutics AG, Basel, Switzerland

"We are a small biotech company without our own wet labs. Sciomics recommended a UK CRO that performed cell culture experiments for our studies. Sciomics seamlessly coordinated the transfer of the biologic materials to their facility and performed the scioCD panel to characterize cell surface markers and cytokines in cell lines treated with our small molecule drug candidates. The Sciomics data were instrumental in supporting our IP protection strategy. Their advice was very helpful and they worked with us to extract the most out of the data. We are very satisfied and highly recommend the Sciomics platform and the team."

Product: scioCD

Our protein profiling services

scioDiscover - In depth profiling on the protein level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…

scioUbi: protein profiling and ubiquitination status

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse more than 1,400 proteins in a single assay.   Features Screen…

scioPhospho: phosphoproteomics and protein profiling service

Discover protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform. Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins. Our scioPhospho protein phosphorylation…

scioCD - Cell Surface Marker and Cytokine Profiling

Immune response profiling Cellular composition and signalling CD-Marker Expression Profiles       Our scioCD profiling service covers 349 different proteins : up to 141 unique CD-marker molecules up to 119 cytokines and chemokines

scioCyto : Cytokine Profiling

scioCyto is a high-content analysis service for a multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell culture supernatants can be screened in a high sensitivity and reproducibility