scioDiscover - Protein Expression Analysis Platform

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1,300 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples. 

 

Application Sample types - Sample requirement Advantages Service includes
       
Signalling Pathway Profiling plasma/serum - 10 ul high-content sample preparation
Immunsystem activation marker profiling tissue - 50 mg 1030 proteins analysed protein extraction
Transcription factor analysis  cells - 5 million   protein concentration measurements
Cancer pathway screening cerebrospinal fluid - 30 ul broad coverage of:  protein quality control
Cell surface marker Profiling cell culture supernatant - 10-20 ml - signalling pathway proteins  
Profiling of apoptotic processes/ apoptosis pathway analysis    - transcription factors sample labelling
  others on request - apoptosis marker sample purification
Disease mechanism profiling   - markers for oxidative stress sample incubation on arrays
Pathway activation in stem cells    - cell surface markers  
Analysis of organ damage/failure models    - cytokines/chemokines raw data acquisition
Response of cells and tissue models to oxidative stress analysis     data normalisation
Profiling proteomic changes for various cell culture conditions   minimal sample requirements data analysis
    native matrixes (non-fractionated/non-depleted) statistical analysis
Molecular initiate event analysis   sensitivity as ELISA or better comprehensive study report
Compound mode-of-action analysis      
Prediction markers for toxicological effects    fully immuno-based assay  
       
Biomarker Discovery   Reliable dual-colour study design  
Drug Target Discovery   high reproducibility, CV < 10%  
       
CD cell surface molecule expression in immune cells        

 

Target coverage - GO annotations

Biological Process Cellular Component Protein Classes 
cellular process (GO:0009987) 438 cell part (GO:0044464) 172 signaling molecule (PC00207) 122
metabolic process (GO:0008152) 410 organelle (GO:0043226) 97 receptor (PC00197) 113
response to stimulus (GO:0050896) 228 extracellular region (GO:0005576) 60 nucleic acid binding (PC00171) 109
biological regulation (GO:0065007) 220 membrane (GO:0016020) 59 hydrolase (PC00121) 77
developmental process (GO:0032502) 172 macromolecular complex (GO:0032991) 47 transcription factor (PC00218) 71
immune system process (GO:0002376) 145 extracellular matrix (GO:0031012) 28 enzyme modulator (PC00095) 67
localization (GO:0051179) 116 cell junction (GO:0030054) 3 cell adhesion molecule (PC00069) 62
multicellular organismal process (GO:0032501) 72 synapse (GO:0045202) 2 defense/immunity protein (PC00090) 52
apoptotic process (GO:0006915) 67     cytoskeletal protein (PC00085) 40
cellular component organization or biogenesis (GO:0071840) 65     oxidoreductase (PC00176) 31
biological adhesion (GO:0022610) 60     kinase (PC00137) 31
reproduction (GO:0000003) 26     transporter (PC00227) 27
locomotion (GO:0040011) 4     transfer/carrier protein (PC00219) 25
        calcium-binding protein (PC00060) 20
        phosphatase (PC00181) 20
        structural protein (PC00211) 18
        extracellular matrix protein (PC00102) 17
        cell junction protein (PC00070) 17
        membrane traffic protein (PC00150) 14

PLEASE NOTE: GO annotation terms may overlap 

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Reference customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

Our service portfolio

scioPhospho: protein profiling and phosphorylation status

scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation. Features 1,300 highly relevant proteins are profiled in a single assay Phosphorylation status combined with protein expression levels…
Read more
scioDiscover - Protein Profiling on expression level

scioDiscover - Protein Profiling on expression level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…
scioCD - Cell surface marker and Cytokine profiling

scioCD - Cell surface marker and Cytokine profiling

  Characterise a certain cell type?   Cell composition in a certain tissue sample?   In-depth profiling of CD-marker expression?   Increase your throughput at high sensitivity levels? You aim…
scioUbi

scioUbi

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse up to 1,000 proteins in a single assay.   Features Screen…
scioCyto : Cytokine profiling

scioCyto : Cytokine profiling

scioCyto : Cytokine profiling scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or…

COVID-19 related activities and services

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News

New article | Prevention of Cyclosp.-A mediated anemia and peritubular loss

| June 2022 | In a study by Robert Labes et al. insights in prevention of cyclosporine mediated anemia and peritubular capillary loss were identified.

Labes, R. et al. Daprodust. prevents cyclosp.-A mediated anemia and peritubular capillary loss. Kidney International 2022 in press.
New article | The Lifestyle Modifications Affect the Endometrial Proteome

| June 2022 | In a study by D. Abdulkhalikova et al. Endometrial Proteome changes werde identified impacted by Lifestyle modification in woman with Polycystic Ovarian Syndrome and Obesity.

Abdulkhalikova, DR. et al. The Lifestyle Modifications and Endometrial Proteome Changes of Women With Polycystic Ovary Syndrome and Obesity. Front. Endocrinol. 2022 in press.
New article | Cycle-dependent biomarkers in endometriosis

| May 2022 | In a study by Maja Pušić et al. Cycle-dependent plasma protein biomarkers for peritoneal endometriosis were identified by our scioDiscover platform.

Pušić, M. et al. Antibody Arrays Identified Cycle-Dependent Plasma Biomarker Candidates of Peritoneal Endometriosis. J. Pers. Med. 202212, 852.
New article | Co-Expression of microRNAs and Proteins in Alzheimer’s Disease

| Jan 2022 | In a study by Valentina di Pietro at Birmingham University our scioDoiscover platform was used to analyse the co-expression of proteins and microRNAs in the brains of Alzheimer's disease patients.

Watson, C. N. et al. Co-Expression Analysis of microRNAs and Proteins in Brain of Alzheimer’s Disease Patients. Cells 11, 163 (2022).
New article | ETV6 levels predict outcome in DLBCL

| Jan 2022 | Erich Piovan and collegues at University of Padua collaborated with Sciomics to study specific biomarkers in FFPE tissue samples of DLBCL patients by custom antibody microarrays.

Marino, D. et al. High ETV6 Levels Support Aggressive B Lymphoma Cell Survival and Predict Poor Outcome in Diffuse Large B-Cell Lymphoma Patients. Cancers 14, (2022).

Testimonials

Dr. Felix Czernilofsky

Department of Medicine V, Heidelberg University Hospital,Heidelberg, Germany

"The scioCyto assay from Sciomics was a tremendous help in characterizing complex supernatants of in vitro tumor microenvironment models. With the professional, fast and friendly support from Ronny and colleagues we were able to confirm and extend our hypotheses. I am very happy to recommend the Sciomics experience!"

Product: scioCyto

 

Dr. Anke König

Clinics for dermatology, venerology and allergology, University Clinics Frankfurt, Frankfurt, Germany

"We enjoyed working with Sciomics on various research projects related to inflammatory skin diseases. The cooperation with Sciomics was just excellent. In addition to processing the samples and compiling the data in a perfectly understandable way, Sciomics GmbH supports us with the entire interpretation and presentation of our results with lots of ideas beyond the usual level of corporate performance. We are looking forward to developing new ideas for further clinical studies together."

Product: scioDiscover

 

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    RT @JPM_MDPI: Article: Antibody Arrays Identified Cycle-Dependent Plasma #Biomarker Candidates of Peritoneal #Endometriosis by Prof. Dr. Te…