Proteomics service for pre-clinical research &

Protein biomarker discovery

Robust, comprehensive & fast

Our high-throughput protein profiling service supports researchers in identifying novel protein biomarkers, understanding disease mechanisms, and accelerating pre-clinical drug development.

Using our proprietary antibody microarray platform, we enable the simultaneous quantification of over 1,500 proteins from minimal sample volumes — ideal for biomarker discovery, pathway analysis, and protein profiling studies.

Partner with Sciomics – your specialized proteomics CRO – and take your biomarker discovery and pre-clinical research to the next level.

   

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Antibody Microarray Workflow for robust proteomics profiling: Illustration depicting the steps involved in protein profiling using antibody microarrays, from protein isolation and sample labeling to antibody immobilization, sample incubation, image acquisition, and data analysis for the scioDiscover platform.

The Assay

  • High-throughput immuno-based protein profiling using complex antibody microarrays
  • Ideal for pre-clinical protein biomarker discovery and pathway analysis
  • Broad coverage of key biological processes and signaling pathways
  • Native sample matrices (non-fractionated / non-depleted)
  • Highest sensitivity and reproducibility (CV <10%, 4 technical replicates) from minimal sample volumes
scioDiscover Proteomic Profiling and PTM Analysis: Diagram illustrating the applications of scioDiscover in biomarker discovery, disease model characterization, pathway activity analysis, drug target discovery, and mode of action studies. As your trusted CRO partner for immunobased proteomics, we offer end-to-end support – from study planning and sample analysis to comprehensive data interpretation. Whether you're exploring disease mechanisms or validating drug effects, our platform delivers actionable insights for your research success.

 

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Data Analysis is included

  • In-depth bioinformatic analysis based on > 6,000 data points per sample
  • Protein interaction and pathway network mapping
  • Functional annotation and biological pathway classification
  • Data integration for different sample types

Application examples

  • Protein biomarker discovery
  • Pre-clinical and translational research
  • Pathway profiling and pathway regulation studies
  • Drug response profiling and mode-of-action studies
  • Patient stratification 
  • Basic research 

Compatible sample types

Our service supports a broad range of sample types – even in very limited amounts:

  • Plasma / Serum
  • Cerebrospinal fluid (CSF)
  • Interstitial fluid (ISF)
  • Urine
  • Saliva
  • Tissue lysates
  • Cell lysates / cultured cells
  • Cell culture supernatants
  • Organoids

If you are working with a different type of biological sample, feel free to contact our scientists – we are generally able to analyse any protein-containing sample

 

scioDiscover - In depth profiling on the protein level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…

scioUbi: protein profiling and ubiquitination status

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse more than 1,400 proteins in a single assay.   Features Screen…

scioPhospho: phosphoproteomics and protein profiling service

Discover protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform. Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins. Our scioPhospho protein phosphorylation…

scioCD - Cell Surface Marker and Cytokine Profiling

Immune response profiling Cellular composition and signalling CD-Marker Expression Profiles       Our scioCD profiling service covers 349 different proteins : up to 141 unique CD-marker molecules up to 119 cytokines and chemokines

scioCyto : Cytokine Profiling

scioCyto is a high-content analysis service for a multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell culture supernatants can be screened in a high sensitivity and reproducibility

Protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform.

Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins.

Our scioPhospho protein phosphorylation analysis service combines protein profiling using scioDiscover with analysis of the corresponding protein phosphorylation status. More than 1,500 proteins are analyzed in parallel, covering key pathways in diseases such as cancer, neurological disorders and organ failure.  This combination provides a comprehensive overview of cellular signaling and pathway activity regulation in your samples.

Thereby, scioPhosho enables a fast and cost-effective in-depth protein and phosphorylation analysis from minute amounts of human biospecimen, organoid models, cell cultur models or animal models. Our full phospho-proteomic analysis service includes expert data analysis and generates knowledge on protein and protein phosphorylation level. This is highly valuable for our clients in preclinical research interested in activity-based protein profiling.

scioPhospho methodology

 

   

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scioPhospho flowchart

The Assay:

  • Phosphorylation and protein expression in a direct comparison in one assay
  • Coverage of key signalling pathways 
  • Analysis of various sample types with minimal sample amounts
  • Native matrices (non-fractionated / non-depleted)
  • 4 replicates for each antibody
  • High reproducibility (CV < 10%)

 

scioPhospho experimental workflow

Applications

  • Pathway analysis / Pathway regulation
  • Mode-of-action studies
  • Drug response profiling
  • Disease mechanism screening
  • Biomarker discovery
  • Validation of predicted protein phosphorylation

Data Analysis is included

  • Comprehensive data analysis using a basis of > 12,000 data points for each sample
  • Differential proteomics and phosphoproteomics
  • Protein interaction network analysis
  • Biological and pathway classification

Phospho-proteomic studies

DUB knockout cell line versus wild type

  • Direct comparison of protein level changes to ubiquitination changes
  • Easy identification of statistically significant changes
  • Immediate overview of molecular changes

sciomics scioUbi Ubiquitination And Protein Profiling case study
Overview of differentially abundant (x-axis) and ubiquitinated (y-axis) proteins from a cell culture study comparing a DUB knockout group with a wild type sample group.

 

Contact us   Overview scio-Ubi

 

scioCyto : Cytokine profiling

scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell cultur supernatants can be used in this robust assay. The complete analysis service comprises all steps from protein extraction from your samples to a comprehensive study report for interpretation of the data.

Features

  • up to 119 cytokines and chemokines analysed in a single assay
  • robust assay with 8 technical replicates
  • most antibodies available for follow-up studies
  • phosphorylation & ubiquitination status on request
 
 

Benefits for your research

  • comprehensive cytokine and chemokine signalling information
  • low sample volumes (10 μL plasma/serum)
  • sensitivity as ELISAs
  • analysis possible from various sample types, including plasma/serum, tissue, cells, supernatants, cerebrospinal fluid (CSF)
  • complete analysis service from protein extraction to bioinformatic data interpretation and a comprehensive study report

scioCyto analysis process

 

Applications

   

Plasma samples 

  • Immune system activity
  • Immune response profile
  • Biomarkers

Tissue samples

  • Microenvironment analysis
  • 3D cell culture models
  • Immune signalling
  • Inflammation status

Cell samples

  • Cellular signalling
  • Immune cell activation
  • Growth/stimuli profiling
  • Supernatant analysis

 

 You need more coverage? Have a look at our scioCD or scioDiscover service!

Contact us  

 

 scioCyto target proteins

 

scioCyto enables a robust profiling of up to 119 cytokines and chemokines in one single assay from minute sample amounts.

scioCyto target cytokines for profiling

 

Chemokines

C-C-Motive


CCL1 ,   CCL2 ,   CCL3 ,   CCL4 ,   CCL5 ,   CCL7 ,   CCL8 ,   CCL11 ,   CCL13 ,   CCL14 ,   CCL15 ,   CCL16 ,   CCL17 ,   CCL18 ,   CCL19 ,   CCL20 ,   CCL21 ,   CCL22 ,   CCL23 ,   CCL24 ,   CCL25 ,   CCL26 ,   CCL27 ,   CCL28
C-X-C motif 

CXCL1 ,   CXCL5 ,   CXCL6 ,   CXCL7 (PPBP) ,   CXCL8 (IL-8) ,   CXCL9 ,
CXCL10 ,   CXCL11 ,   CXCL12 (SDF-1) ,   CXCL13 ,   CXCL14 ,   CXCL16 
C motif XCL1
C-X3-C motif CX3CL1
   

Cytokines

Interferons IFNA1 ,   IFNG ,   IFNL1 ,   IFNL2 ,   IFNL3

Interleukins



IL-1A ,   IL-1B ,   IL-2 ,   IL-3 ,   IL-4 ,   IL-5 ,   IL-6 ,   IL-7 ,   IL-8 (CXCL8) ,   IL-9 ,
IL-10 ,   IL-12p70 ,   IL-12B ,   IL-13 ,   IL-15 ,   IL-16 ,   IL-17 ,   IL-17B ,   IL-17C ,   IL-17F ,   IL-18 ,   IL-19 ,
IL-20 ,   IL-22 ,   IL-23A ,   IL-25 ,   IL-27A ,   IL-28A (IFNL2) ,   IL-28B (IFNL3) ,   IL-29 (IFN-L1) ,
IL-31 ,   IL-32 ,   IL-33 ,   IL-34 ,   IL-36G ,   IL-37
TNF-Family





TNFA (TNF) ,   TNFB (LTA) ,
TNFSF4 (CD252) ,   TNFSF5 (CD154, CD40L) ,  
TNFSF6 (FASLG, CD95-L) 
,   TNFSF7 (CD70, CD27-L) ,   TNFSF8 (CD153, CD30-L) ,
TNFSF10 (TRAIL, CD253) ,   TNFSF11 (CD254, OPG-L) ,   TNFSF12 (APO3-L) ,
TNFSF13 (APRIL, CD256) ,   TNFSF13B (BAFF, CD257) ,
TNFSF14 (CD258, HVEM-L) ,   TNFSF18 (AITRL) 
   

Cytokine / growth factor activity

Colony-stimulating factors (CSF) M-CSF ,   GM-CSF ,   G-CSF
TGF-Family TGFB1 ,   TGFB2 ,   TGFB3
VEGF-Family VEGF165/VEGF121 ,   VEGFA ,   VEGFC
Others










CNTF ,   CRLF2 ,   CYTL1,
FGF2 ,   FLT3L ,
GDF2 ,   GDF15 ,   GRN,
HGF ,   HMGB1 ,
IGF1 ,   INHBA ,
LIF ,
MIF ,
NGF-beta ,
ONCM ,   OSTP ,
PLF4 ,
TSLP

 

Contact us  

 Need more coverage? Have a look at our scioCD or scioDiscover service!

Protein & ubiquitination profiling

scioUbi is a high-content protein expression and ubiquitination level analysis.
With scioUbi you can analyse more than 1,400 proteins in a single assay.

 

Features

  • Screen for differential ubiquitination status
    in more than 1,400 proteins in parallel in a single assay
  • 4 technical replicates per sample
  • Comprehensive data analysis and presentation including
    an individualised report
  • Customisation of ubiquitin detection on request

Benefits for your research

  • Two in one: High-content protein expression and ubiquitination level analysis in a single assay
  • Proteins selected for biomedical studies: Central regulators (e.g. mTor, p53), transcription factors, receptors
  • Minimal sample (e.g. tissue or cell culture samples) amounts needed for complete analysis
sciomics scioUbi Ubiquitination And Protein Profiling Biological processes selection of proteins
Biological processes
  sciomics scioUbi Ubiquitination And Protein Profiling Cell Cycle proteins coveredCell cycle: proteins in green are covered by the analysis

 

Applications

Biological effects of

  • protein degradation in health and disease
  • protein activity changes
  • protein interaction alterations
  • drug response (e.g. proteasome enhancers)

Others

  • Interplay between ubiquitination and phosphorylation (see scioPhospho)
  • Neurodegenerative diseases
  • Progression of ageing
  • Cell cycle regulation
  • Proteasome activity
  • Transcriptional regulation
  • p53 pathway regulation
  • Alzheimer pathway regulation

 

Contact us

 

Fluorescence image of a scioCD antibody microarray for the profiling of cytokines and cell surface markers

Immune response profiling

Cellular composition and signalling

CD-Marker Expression Profiles

 

 

 

Our scioCD profiling service covers 349 different proteins:

Protein interaction network of 119 cytokines / chemokines and 140 cell surface markers screened in parallel by our scioCD protein profiling service

  • up to 141 unique CD-marker molecules
  • up to 119 Cytokines/Chemokines
  • other relevant proteins such as various HLAs and p53


All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of samples and signalling in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently.

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

 

 

      Analysis
Service
      Sample
Types
      Contact
us

 

  

Applications

  • profiling of cell differentiation status
  • stem cell differentiation 
  • investigate soluble CD-molecules for e.g. allograft rejection
  • inflammatory response profiling
  • immuno-oncology profiling of check-point inhibitor targets
  • characterisation of tissue-isolated primary cells
  • immune-cell surface marker profiling on
    • various B-cells 
    • stimulated T-cells
    • patient derived T-cells
    • patient derived dendritic cells
  • oncology profiling for new biomarkers e.g. Leukemia
  • tumor micro-environment profiling
  • profile response to stimuli of cell lines
  • evaluate protein expression changes in silencing/overexpression  experiments
 

Advantages

  • high content: up to 349 proteins are analysed
  • broad coverage and parallel analysis of
    • CD molecules 
    • cytokines and chemokines
    • other relevant molecules (HLAs, p53)
  • minimal sample requirements
  • native matrixes ( non-fractionated / non-depleted )
  • sensitivity as ELISA or better
  • fully immuno-based assay
  • reliable dual-colour study design
  • high reproducibility, CV < 10%
  • most proteins are covered redundantly by two or more antibodies
  • each antibody is presented in four replicates

The assay

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

  • plasma  or serum
  • fresh frozen tissue samples
  • cellular samples
  • cell culture samples
  • cerebrospinal fluid (CSF)
  • additional sample types on request

  

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report


 

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Our protein profiling services

scioDiscover - In depth profiling on the protein level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…

scioUbi: protein profiling and ubiquitination status

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse more than 1,400 proteins in a single assay.   Features Screen…

scioPhospho: phosphoproteomics and protein profiling service

Discover protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform. Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins. Our scioPhospho protein phosphorylation…

scioCD - Cell Surface Marker and Cytokine Profiling

Immune response profiling Cellular composition and signalling CD-Marker Expression Profiles       Our scioCD profiling service covers 349 different proteins : up to 141 unique CD-marker molecules up to 119 cytokines and chemokines

scioCyto : Cytokine Profiling

scioCyto is a high-content analysis service for a multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell culture supernatants can be screened in a high sensitivity and reproducibility