scioPhospho: phospho-proteomic protein profiling service

Protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform.

Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins.

Our scioPhospho protein phosphorylation analysis service combines protein profiling using scioDiscover with analysis of the corresponding protein phosphorylation status. More than 1,500 proteins are analyzed in parallel, covering key pathways in diseases such as cancer, neurological disorders and organ failure.  This combination provides a comprehensive overview of cellular signaling and pathway activity regulation in your samples.

Thereby, scioPhosho enables a fast and cost-effective in-depth protein and phosphorylation analysis from minute amounts of human biospecimen, organoid models, cell cultur models or animal models. Our full phospho-proteomic analysis service includes expert data analysis and generates knowledge on protein and protein phosphorylation level. This is highly valuable for our clients in preclinical research interested in activity-based protein profiling.

scioPhospho methodology

 

   

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scioPhospho flowchart

The Assay:

  • Phosphorylation and protein expression in a direct comparison in one assay
  • Coverage of key signalling pathways 
  • Analysis of various sample types with minimal sample amounts
  • Native matrices (non-fractionated / non-depleted)
  • 4 replicates for each antibody
  • High reproducibility (CV < 10%)

 

scioPhospho experimental workflow

Applications

  • Pathway analysis / Pathway regulation
  • Mode-of-action studies
  • Drug response profiling
  • Disease mechanism screening
  • Biomarker discovery
  • Validation of predicted protein phosphorylation

Data Analysis is included

  • Comprehensive data analysis using a basis of > 12,000 data points for each sample
  • Differential proteomics and phosphoproteomics
  • Protein interaction network analysis
  • Biological and pathway classification

Phospho-proteomic studies

Publications using scioPhospho

Article | Prevention of anemia and peritubular capillary loss

| October 2022 | Dapr. protects against induced kidney toxicity and anemia by preventing protein phosphorylation changes, preserving microcirculation, and maintaining hemoglobin levels.
Sciomics contributed to this study by analyzing mouse kidney samples using the scioPhospho proteomic assay.

Labes, R., Brinkmann, L. , Kulow, V.A., et al. Dapr. prevents cyclosp.-A-mediated anemia and peritubular capillary loss. Kidney Int. 2022;102(4):750-765.

Article | Protection from ischemia reperfusion injury-induced inflammation and fibrosis in C5aR2-deficient mice

| June 2018 | Complement component 5a receptor 2 (C5AR2) deficient mice are protected from inflammation and fibrosis in response to ischemia reperfusion injury. Our scioPhospho platform supported scientists at the Medical School Hannover to elucidate the underlying mechanism. 

Thorenz, A., Derlin, K., Schröder, C., et al. 2018 Enhanced activation of interleukin-10, heme oxygenase-1, and AKT in C5aR2-deficient mice is associated with protection from ischemia reperfusion injury–induced inflammation and fibrosis. Kidney International 94, 741–755.

Testimonials phosphorylation analysis

Dr. Bettina Grötsch

Department of Medicine 3 - Rheumatology and Immunology, University Clinics Erlangen, Erlangen, Germany

"Our lab is working with human osteoclast that were isolated and differentiated from human peripheral blood. Therefore, we were quite limited in cell numbers and protein content for our planned Phosphoprotein analyses. Nonetheless, Sciomics and especially Camille Lowy helped us a lot to test and improve our protein isolation techniques to achieve some interesting phosphoproteome data in human osteoclasts. We were really impressed by the detailed data report and their willingness to discuss and reanalyze the data repeatedly until we got the best results out of our measurements. Even several month after their first report, we could ask for help and suggestions with an immediate reply.  The phosphoproteome analyses completed our preliminary project data and are now published in Frontiers Immunology.  Altogether, we were very happy with the service of Sciomics and special thanks to Camille Lowy and Christoph Schröder who guided us through the whole process."

Product: scioPhospho
Publication: Grötsch B. et al. 2022 Front. Immunol. 13:958974 

Dr. Leonardo Guasti

Centre for Endocrinology, William Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom

"We were satisfied with the service provided by Sciomics back in 2017, so when it came to study a newly discovered secreted protein, for which little biology is known, we decided to engage again with them. Indeed, the phosphorylation and protein expression level data provided by Sciomics was of great help in assessing modulation of pathways initiated by adrenal cortex cells treated with this protein. Crucially, the Sciomics team actively engaged with us during both the planning of the experiments and subsequently in data analysis. This comprehensive data set has truly put us in the right path to dig into the role of a newly discovered protein in adrenocortical pathobiology."

Product: scioPhospho

 

Explore our protein profiling services

scioDiscover - In depth profiling on the protein level
Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…

scioUbi: protein profiling and ubiquitination status
Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse more than 1,400 proteins in a single assay.   Features Screen…

scioPhospho: phosphoproteomics and protein profiling service
Discover protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform. Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins. Our scioPhospho protein phosphorylation…

scioCD - Cell Surface Marker and Cytokine Profiling
Immune response profiling Cellular composition and signalling CD-Marker Expression Profiles       Our scioCD profiling service covers 349 different proteins : up to 141 unique CD-marker molecules up to 119 cytokines and chemokines

scioCyto : Cytokine Profiling
scioCyto is a high-content analysis service for a multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell culture supernatants can be screened in a high sensitivity and reproducibility
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