Protein biomarker discovery & development

Accelerating Biomarker Research Through Robust Immuno-based Proteomics

Reliable biomarkers are essential for  early disease detection, patient stratification, and treatment monitoring. Among biomarker types, proteins offer significant advantages, enabling cost-efficient and high-throughput measurements often within minutes using standard immuno-assays or lateral flow devices. Sciomics is a leading Biomarker CRO dedicated to advancing protein biomarker research for precison medicine. We provide a comprehensive and reproducible approach to biomarker discovery and validation utilizing our scioDiscover platform. Our expertise assists researchers across diverse fields, ensuring the generation of robust and clinically relevant results.

Enabling reliable & translational Biomarker Discovery

Our scioDiscover platform ensures:

  • Robust Data: Ensure data reliability and high reproducibility with four technical replicates per target protein and sample.
  • Broad Coverage: Unmatched multiplexing and sensitivity for diverse, complex samples.
  • Effortless translation: Seamless integration into diagnostic assays and lab tests due to immuno-based methodology..
With these benefits, Sciomics is your ideal partner for biomarker discovery and development.
Sciomics supports the discovery and development of a variety of different protein biomarker types such as drug response markers, stratification markers, CDx markers, prognostic biomarkers or diagnostic biomarkers.

Our track record in biomarker discovery

We have performed successful biomarker studies in diverse research areas, including:

  • Acute Kidney Injury (AKI)
  • Assessing Dengue Fever Severity
  • Neurodegenerative Diseases (Alzheimer’s & Parkinson’s)
  • MCDS (Metaphyseal Chondrodysplasia, Schmid Type)
  • COVID-19 disease severity
  • Endometrial Cancer
  • Endometriosis
  • Infectious Diseases

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Comprehensive Scientific Support for Protein Biomarker Discovery & Development

 We offer tailored expert guidance throughout the entire process, from study design and protein analysis to data interpretation and biomarker validation. Choose Sciomics as your preferred Biomarker CRO for development projects. 

Sciomics supports your protein biomarker development project via the steps study design, sample selection, sample analysis, data analysis, data interpretation as well as validation.

1. Study design:  Our experts help perfect your experimental biomarker study setup, sample, and control selection for robust, meaningful results.

2. Sample selection: We analyze diverse sample types (plasma, serum, urine, CSF, ISF, PBMC, tissue, cells, etc.), requiring minimal input amounts.

3. Sample analysis: Our experts process your samples from protein extraction to incubation, delivering results quickly (within few weeks).

4. Data analysis: Comprehensive bioinformatics and machine learning provide actionable insights, ROC-curves, and robust, interpretable biomarker panels for routine testing.

5. Data interpretation: Detailed reports and guidance to support your validation and follow-up strategies.

6. Validation:  Our antibody platform facilitates easy transfer to standard immuno-assays, accelerating validation and diagnostic assay development. 

 

Biomarker discovery based on our antibody-based discovery platform ensures a straight-forward translation into other immuno-assays for validation, accelerating and de-risking the development of diagnostic assays. 

 

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Validating scioDiscover Biomarkers: Proven Success

Direct Validation: Easily confirm scioDiscover findings using common immuno-assays like ELISA.

Accelerated Implementation: Speed up the journey from biomarker discovery to clinical use and diagnostics.

Exceptional Correlation: Strong agreement (Pearson’s r > 0.9) between discovery and validation data.

 

Correlation of r > 0.9 for data generated by the scioDiscover platform and biomarker measurement in a routine clinical laboratory

An immunobased discovery approach for protein biomarkers enables an easy translation to other immunobased platforms such as ELISA for validation.

 

Selected projects with external validation

Roche: Validated two biomarkers using their Elecsys platform ( poster)

AbbVie: Confirmed biomarkers from a small cohort on an internal platform (paper)

University Hospital Heidelberg: Validated CRP in their clinical laboratory; additional marker confirmed by ELISA in our lab (paper)

Hannover Medical School: Validation of tissue proteomics/phosphoproteomics in a mouse model by Western Blotting (paper)

Charité: Verified phosphorylation state via Western blot/IHC in a tissue study (paper)

scioDiscover: Protein profiling & biomarker discovery

Proteomics service for pre-clinical research &

Protein biomarker discovery

Robust, comprehensive & fast

Our high-throughput protein profiling service supports researchers in identifying novel protein biomarkers, understanding disease mechanisms, and accelerating pre-clinical drug development.

Using our proprietary antibody microarray platform, we enable the simultaneous quantification of over 1,500 proteins from minimal sample volumes — ideal for biomarker discovery, pathway analysis, and protein profiling studies.

Partner with Sciomics – your specialized proteomics CRO – and take your biomarker discovery and pre-clinical research to the next level.

   

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Antibody Microarray Workflow for robust proteomics profiling: Illustration depicting the steps involved in protein profiling using antibody microarrays, from protein isolation and sample labeling to antibody immobilization, sample incubation, image acquisition, and data analysis for the scioDiscover platform.

The Assay

  • High-throughput immuno-based protein profiling using complex antibody microarrays
  • Ideal for pre-clinical protein biomarker discovery and pathway analysis
  • Broad coverage of key biological processes and signaling pathways
  • Native sample matrices (non-fractionated / non-depleted)
  • Highest sensitivity and reproducibility (CV <10%, 4 technical replicates) from minimal sample volumes
scioDiscover Proteomic Profiling and PTM Analysis: Diagram illustrating the applications of scioDiscover in biomarker discovery, disease model characterization, pathway activity analysis, drug target discovery, and mode of action studies. As your trusted CRO partner for immunobased proteomics, we offer end-to-end support – from study planning and sample analysis to comprehensive data interpretation. Whether you're exploring disease mechanisms or validating drug effects, our platform delivers actionable insights for your research success.

 

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Data Analysis is included

  • In-depth bioinformatic analysis based on > 6,000 data points per sample
  • Protein interaction and pathway network mapping
  • Functional annotation and biological pathway classification
  • Data integration for different sample types

Application examples

  • Protein biomarker discovery
  • Pre-clinical and translational research
  • Pathway profiling and pathway regulation studies
  • Drug response profiling and mode-of-action studies
  • Patient stratification 
  • Basic research 

Compatible sample types

Our service supports a broad range of sample types – even in very limited amounts:

  • Plasma / Serum
  • Cerebrospinal fluid (CSF)
  • Interstitial fluid (ISF)
  • Urine
  • Saliva
  • Tissue lysates
  • Cell lysates / cultured cells
  • Cell culture supernatants
  • Organoids

If you are working with a different type of biological sample, feel free to contact our scientists – we are generally able to analyse any protein-containing sample

 

scioPhospho: phospho-proteomic protein profiling service

Protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform.

Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins.

Our scioPhospho protein phosphorylation analysis service combines protein profiling using scioDiscover with analysis of the corresponding protein phosphorylation status. More than 1,500 proteins are analyzed in parallel, covering key pathways in diseases such as cancer, neurological disorders and organ failure.  This combination provides a comprehensive overview of cellular signaling and pathway activity regulation in your samples.

Thereby, scioPhosho enables a fast and cost-effective in-depth protein and phosphorylation analysis from minute amounts of human biospecimen, organoid models, cell cultur models or animal models. Our full phospho-proteomic analysis service includes expert data analysis and generates knowledge on protein and protein phosphorylation level. This is highly valuable for our clients in preclinical research interested in activity-based protein profiling.

scioPhospho methodology

 

   

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scioPhospho flowchart

The Assay:

  • Phosphorylation and protein expression in a direct comparison in one assay
  • Coverage of key signalling pathways 
  • Analysis of various sample types with minimal sample amounts
  • Native matrices (non-fractionated / non-depleted)
  • 4 replicates for each antibody
  • High reproducibility (CV < 10%)

 

scioPhospho experimental workflow

Applications

  • Pathway analysis / Pathway regulation
  • Mode-of-action studies
  • Drug response profiling
  • Disease mechanism screening
  • Biomarker discovery
  • Validation of predicted protein phosphorylation

Data Analysis is included

  • Comprehensive data analysis using a basis of > 12,000 data points for each sample
  • Differential proteomics and phosphoproteomics
  • Protein interaction network analysis
  • Biological and pathway classification

Phospho-proteomic studies

Our protein profiling services

scioDiscover - In depth profiling on the protein level
Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…

scioUbi: protein profiling and ubiquitination status
Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse more than 1,400 proteins in a single assay.   Features Screen…

scioPhospho: phosphoproteomics and protein profiling service
Discover protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform. Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins. Our scioPhospho protein phosphorylation…

scioCD - Cell Surface Marker and Cytokine Profiling
Immune response profiling Cellular composition and signalling CD-Marker Expression Profiles       Our scioCD profiling service covers 349 different proteins : up to 141 unique CD-marker molecules up to 119 cytokines and chemokines

scioCyto : Cytokine Profiling
scioCyto is a high-content analysis service for a multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell culture supernatants can be screened in a high sensitivity and reproducibility

More Articles ...

  1. scioUbi: protein profiling and ubiquitination status
  2. scioCyto : Cytokine Profiling
  3. scioCD - Cell Surface Marker and Cytokine Profiling
  4. scioDiscover - Protein Profiling on the Expression Level