Start your career @sciomics

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower cost through a highly-parallel protein expression level analysis using our Scio-Discover Antibody Microarray Platform. Furthermore, you may link to and verify your Genomics and Transcriptomics data with protein expression levels.

Applications:

Pre-clinical Toxicology Studies
- rodents such as mice* and rats*
- cell culture models
- tissue culture models
Drug target identification and verification
Mechanistic studies for various indications
Profile cytokine and inflammatory status
Protein Biomarker identification and verification

Key advantages:

High multiplexity (more than 1,400 proteins in parallel)
Low sample consumption
Investigation of human and murine samples*
Fast turnaround time, as low as two weeks**
No need for laborious depletion or fractionation of your samples
High sensitivity (as sensitive as an ELISA or better)
High reproducibility (CV < 10%)
Various sample types can be investigated
- Plasma or Serum
- fresh-frozen tissue samples
- Cell culture samples
- Cerebrospinal fluid (CSF)

Overview
Scio-Discover Biomarker
Discovery Contact
us

Reference Customers:

Dr. Ghaith Bakdash, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands
Dr. Anke Dienelt, Julius Wolff Institut, Charité, Berlin, Germany
Dr. Eric Eldering, Academic Medical Center, Amsterdam, The Netherlands
Dr. Reece Marillier, Ludwig Institute for Cancer Research Ltd, Brussels, Belgium
Prof. Jose L. Peiró, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, USA

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

** Depending on study design and sample numbers.

You have identified a compound with the desired effect ?

You are looking for its targets ?

Which additional pathways are affected ?

Which proteins are altered in a diseased tissue ?

Get a complete picture of protein expression in multiple pathways !

In-depth knowledge about protein expression levels is crucial for the discovery and evaluation of new drug targets. Our Scio-Discover service is fully immuno-based and covers proteins involved in key disease and signaling pathways as well as secreted proteins. Only minimal sample amounts are required for highly parallel protein analysis. There is no need for sample fractionation or depletion. Our platform is capable of processing most biological samples without extensive sample preparation, saving valuable time and resources.

Applications

Drug target identification and verification studies
Mechanistic studies for various indications
Correlation of next-gen sequencing (NGS) data with protein expression levels
Profile cytokine and inflammatory status of your samples

Key Advantages

Low sample consumption, for example*
- Plasma sample: 5µl
- Tissue: 1mg (fresh-frozen)
- Cells: 100,000
- CSF: 5µl
Investigation of human, murine and rat samples**
Fast turnaround time, as little as two weeks***
No need for laborious sample depletion or fractionation
High sensitivity (as sensitive as or more sensitive than ELISA)
High reproducibility (CV < 10%)
Various sample types can be analyzed
- Blood serum or plasma
- Tissue
- Cell culture
- Cerebrospinal fluid (CSF)

Service Features

Study design
Protein extraction and purification
Protein quantification
Protein labelling with high-performance fluorescent dyes
Automated array incubation and handling
Array scanning and data acquisition
Data analysis according to your requirements
Customized study report

Overview
Scio-Discover Biomarker
Discovery Contact
us

*Minimum quantity. These values may vary depending on the sample type, study design, storage conditions. and additional parameters.

_{** Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].}

_{*** Depending on study design and sample numbers.}

Fluorescence image of a scioCD antibody microarray for the profiling of cytokines and cell surface markers

Immune response profiling

Cellular composition and signalling

CD-Marker Expression Profiles

Our scioCD profiling service covers 349 different proteins:

Protein interaction network of 119 cytokines / chemokines and 140 cell surface markers screened in parallel by our scioCD protein profiling service

up to 141 unique CD-marker molecules
up to 119 Cytokines/Chemokines
other relevant proteins such as various HLAs and p53

All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of samples and signalling in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently.

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

      Analysis
Service       Sample
Types       Contact
us

Applications

profiling of cell differentiation status
stem cell differentiation
investigate soluble CD-molecules for e.g. allograft rejection
inflammatory response profiling
immuno-oncology profiling of check-point inhibitor targets
characterisation of tissue-isolated primary cells
immune-cell surface marker profiling on
- various B-cells
- stimulated T-cells
- patient derived T-cells
- patient derived dendritic cells
oncology profiling for new biomarkers e.g. Leukemia
tumor micro-environment profiling
profile response to stimuli of cell lines
evaluate protein expression changes in silencing/overexpression experiments

Advantages

high content: up to 349 proteins are analysed
broad coverage and parallel analysis of
- CD molecules
- cytokines and chemokines
- other relevant molecules (HLAs, p53)
minimal sample requirements
native matrixes ( non-fractionated / non-depleted )
sensitivity as ELISA or better
fully immuno-based assay
reliable dual-colour study design
high reproducibility, CV < 10%
most proteins are covered redundantly by two or more antibodies
each antibody is presented in four replicates

The assay

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

plasma or serum
fresh frozen tissue samples
cellular samples
cell culture samples
cerebrospinal fluid (CSF)
additional sample types on request

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

definition of an appropriate study design
sample preparation
protein extraction
protein concentration measurements
protein quality control
sample labelling
sample purification
incubation of the samples on antibody microarrays
microarray scanning
raw data acquisition
data normalisation
data analysis including cluster analysis
statistical testing for differentially abundant proteins
comprehensive study report

scioCyto : Cytokine profiling

scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell cultur supernatants can be used in this robust assay. The complete analysis service comprises all steps from protein extraction from your samples to a comprehensive study report for interpretation of the data.

Features

up to 119 cytokines and chemokines analysed in a single assay
robust assay with 8 technical replicates
most antibodies available for follow-up studies
phosphorylation & ubiquitination status on request

Benefits for your research

comprehensive cytokine and chemokine signalling information
low sample volumes (10 μL plasma/serum)
sensitivity as ELISAs
analysis possible from various sample types, including plasma/serum, tissue, cells, supernatants, cerebrospinal fluid (CSF)
complete analysis service from protein extraction to bioinformatic data interpretation and a comprehensive study report

scioCyto analysis process

Applications

Plasma samples

Immune system activity
Immune response profile
Biomarkers

Tissue samples

Microenvironment analysis
3D cell culture models
Immune signalling
Inflammation status

Cell samples

Cellular signalling
Immune cell activation
Growth/stimuli profiling
Supernatant analysis

You need more coverage? Have a look at our scioCD or scioDiscover service!

scioCyto target proteins

scioCyto enables a robust profiling of up to 119 cytokines and chemokines in one single assay from minute sample amounts.

Chemokines
C-C-Motive	CCL1 , CCL2 , CCL3 , CCL4 , CCL5 , CCL7 , CCL8 , CCL11 , CCL13 , CCL14 , CCL15 , CCL16 , CCL17 , CCL18 , CCL19 , CCL20 , CCL21 , CCL22 , CCL23 , CCL24 , CCL25 , CCL26 , CCL27 , CCL28
C-X-C motif	CXCL1 , CXCL5 , CXCL6 , CXCL7 (PPBP) , CXCL8 (IL-8) , CXCL9 , CXCL10 , CXCL11 , CXCL12 (SDF-1) , CXCL13 , CXCL14 , CXCL16
C motif	XCL1
C-X3-C motif	CX3CL1

Cytokines
Interferons	IFNA1 , IFNG , IFNL1 , IFNL2 , IFNL3
Interleukins	IL-1A , IL-1B , IL-2 , IL-3 , IL-4 , IL-5 , IL-6 , IL-7 , IL-8 (CXCL8) , IL-9 , IL-10 , IL-12p70 , IL-12B , IL-13 , IL-15 , IL-16 , IL-17 , IL-17B , IL-17C , IL-17F , IL-18 , IL-19 , IL-20 , IL-22 , IL-23A , IL-25 , IL-27A , IL-28A (IFNL2) , IL-28B (IFNL3) , IL-29 (IFN-L1) , IL-31 , IL-32 , IL-33 , IL-34 , IL-36G , IL-37
TNF-Family	TNFA (TNF) , TNFB (LTA) , TNFSF4 (CD252) , TNFSF5 (CD154, CD40L) , TNFSF6 (FASLG, CD95-L) , TNFSF7 (CD70, CD27-L) , TNFSF8 (CD153, CD30-L) , TNFSF10 (TRAIL, CD253) , TNFSF11 (CD254, OPG-L) , TNFSF12 (APO3-L) , TNFSF13 (APRIL, CD256) , TNFSF13B (BAFF, CD257) , TNFSF14 (CD258, HVEM-L) , TNFSF18 (AITRL)

Cytokine / growth factor activity
Colony-stimulating factors (CSF)	M-CSF , GM-CSF , G-CSF
TGF-Family	TGFB1 , TGFB2 , TGFB3
VEGF-Family	VEGF165/VEGF121 , VEGFA , VEGFC
Others	CNTF , CRLF2 , CYTL1, FGF2 , FLT3L , GDF2 , GDF15 , GRN, HGF , HMGB1 , IGF1 , INHBA , LIF , MIF , NGF-beta , ONCM , OSTP , PLF4 , TSLP

Need more coverage? Have a look at our scioCD or scioDiscover service!

Protein & ubiquitination profiling

scioUbi is a high-content protein expression and ubiquitination level analysis.
With scioUbi you can analyse more than 1,400 proteins in a single assay.

Features

Screen for differential ubiquitination status
in more than 1,400 proteins in parallel in a single assay
4 technical replicates per sample
Comprehensive data analysis and presentation including
an individualised report
Customisation of ubiquitin detection on request

Benefits for your research

Two in one: High-content protein expression and ubiquitination level analysis in a single assay
Proteins selected for biomedical studies: Central regulators (e.g. mTor, p53), transcription factors, receptors
Minimal sample (e.g. tissue or cell culture samples) amounts needed for complete analysis

Biological processes

sciomics scioUbi Ubiquitination And Protein Profiling Cell Cycle proteins covered

Cell cycle: proteins in green are covered by the analysis

Applications

Biological effects of

protein degradation in health and disease
protein activity changes
protein interaction alterations
drug response (e.g. proteasome enhancers)

Others

Interplay between ubiquitination and phosphorylation (see scioPhospho)
Neurodegenerative diseases
Progression of ageing
Cell cycle regulation
Proteasome activity
Transcriptional regulation
p53 pathway regulation
Alzheimer pathway regulation

DUB knockout cell line versus wild type

Direct comparison of protein level changes to ubiquitination changes
Easy identification of statistically significant changes
Immediate overview of molecular changes

sciomics scioUbi Ubiquitination And Protein Profiling case study
Overview of differentially abundant (x-axis) and ubiquitinated (y-axis) proteins from a cell culture study comparing a DUB knockout group with a wild type sample group.

Protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform.

Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins.

Our scioPhospho protein phosphorylation analysis service combines protein profiling using scioDiscover with analysis of the corresponding protein phosphorylation status. More than 1,500 proteins are analyzed in parallel, covering key pathways in diseases such as cancer, neurological disorders and organ failure. This combination provides a comprehensive overview of cellular signaling and pathway activity regulation in your samples.

Thereby, scioPhosho enables a fast and cost-effective in-depth protein and phosphorylation analysis from minute amounts of human biospecimen, organoid models, cell cultur models or animal models. Our full phospho-proteomic analysis service includes expert data analysis and generates knowledge on protein and protein phosphorylation level. This is highly valuable for our clients in preclinical research interested in activity-based protein profiling.

scioPhospho methodology

   Article in
our research blog       Discuss your project
with a scientist

The Assay:

Phosphorylation and protein expression in a direct comparison in one assay
Coverage of key signalling pathways
Analysis of various sample types with minimal sample amounts
Native matrices (non-fractionated / non-depleted)
4 replicates for each antibody
High reproducibility (CV < 10%)

Applications

Pathway analysis / Pathway regulation
Mode-of-action studies
Drug response profiling
Disease mechanism screening
Biomarker discovery
Validation of predicted protein phosphorylation

Data Analysis is included

Comprehensive data analysis using a basis of > 12,000 data points for each sample
Differential proteomics and phosphoproteomics
Protein interaction network analysis
Biological and pathway classification

Phospho-proteomic studies

Characterisation of a Parkinson Organoid Model in terms of protein secretion as well as intracellular effects
Poster presented at ADPD 2022
Phosphoproteomic anaylsis of osteoclasts differentiated from human peripheral blood
Front. Immunol. 13:958974
Protection of kidney toxicity and anemia by preventing protein phosphorylation changes
Kidney Int. (2022)102(4):750-765.
Characterisation of protective effect of Complement component 5a receptor 2 in ischemia reperfusion injury
Kidney International (2018) 94, 741–755.
Analysis of BET and PARP inhibitor synergy in Small Cell Lung Cancer cellular model
Int J Mol Sci (2020) 21

scioDiscover: high-content protein profiling of 1,500 proteins

scioDiscover - In depth profiling on the protein level

scioUbi: protein profiling and ubiquitination status

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse more than 1,400 proteins in a single assay. Features Screen…

scioPhospho: phosphoproteomics and protein profiling service

Discover protein abundance and phosphorylation analysis in one step by our high-throughput scioPhospho platform. Simultaneous detection of protein levels & phosphorylation status of > 1,500 proteins. Our scioPhospho protein phosphorylation…

scioCD - Cell Surface Marker and Cytokine Profiling

Immune response profiling Cellular composition and signalling CD-Marker Expression Profiles Our scioCD profiling service covers 349 different proteins : up to 141 unique CD-marker molecules up to 119 cytokines and chemokines

scioCyto : Cytokine Profiling

scioCyto is a high-content analysis service for a multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell culture supernatants can be screened in a high sensitivity and reproducibility

Start your career @sciomics

Reference Customers:

Applications

Key Advantages

Service Features

Applications

Advantages