scioDiscover - Protein Expression Analysis

Protein expression profiling with protein arrays

Our scioDiscover service enables high-content, immuno-based protein profiling of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1030 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples.

 

Features

  • Major pathways, secreted proteins, cell surface and inflammatory markers are covered
  • 1,300 proteins are profiled
  • Broad spectrum of indications covered such as oncology and organ failure
  • Robust dual-colour study design
  • Including full data analysis and study report
  • Studies possible in human, murine and rat samples*

 

 Array
 content
    Protein
 profiling
    Biomarker
 Discovery
     Target
 Discovery
     Contact
 us

 

 

Applications

  • Protein expression level analysis
  • Signalling Pathway Profiling
  • Immunsystem activation marker profiling
  • Transcription factor analysis 
  • Cancer pathway screening
  • Cell surface marker profiling
  • Profiling of apoptotic processes/ apoptosis pathway analysis 

 

  • Disease mechanism profiling
  • Pathway activation in stem cells 
  • Analysis of organ damage/failure models 
  • Response of cells and tissue models to oxidative stress analysis
  • Profiling proteomic changes for various cell culture conditions

 

  • Molecular initiate event analysis
  • Compound mode-of-action analysis
  • Prediction markers for toxicological effects 

 

 

  • CD cell surface molecule expression in immune cells  

 

 

 

 

Advantages

  • high-content protein profiling of 1030 proteins 
  • broad coverage of:
    • signalling pathway proteins
    • transcription factors
    • apoptosis marker
    • markers for oxidative stress
    • cell surface markers
    • cytokines & chemokines

 

  • minimal sample requirements
  • native matrixes (non-fractionated / non-depleted)
  • sensitivity as ELISA or better

 

  • fully immuno-based assay

 

  • reliable dual-colour study design
  • technical replication ( 4 replicates for each antibody )
  • high reproducibility ( CV < 10% )

 

  • experienced partners with more than ten years of experience in conducting biomedical antibody array studies

 

 

Save time, costs and valuable sample

 

 

 

Reference Customers:

  • Prof. Dr. Faikah Güler, Molecular mechanisms of transplant dysfunction, Medical School Hanover, Hanover, Germany
  • Julian Kamhieh-Milz, Research Center ImmunoSciences, Charité, Berlin, Germany
  • Prof. Jose L. Peiró, Cincinnati Fetal Center Director, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, USA
 

Case studies:

Bladder Cancer

Acute Kidney Injury


 

Which sample types can be analysed ?

With the scioDiscover service a variety of samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request
  • studies possible in samples from human, mouse or rat*

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

Sample-to-result serviceAntibody microarray workflow

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

 

scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation.

Features

  • 1,300 highly relevant proteins are profiled in a single assay
  • Phosphorylation status combined with protein expression levels
  • Tyrosine, serine and threonine phosphorylation
  • Analysis of individual phosphorylation types available
  • "Sample-to-result" service
  • Comprehenisive data analysis and presentation
  • Individualised study report
Protein abundance levels and phosphorylation status for one individual protein out of 900 proteins tested

Advantages

  • High-content screening of proteins and phosphorylation is time and ressource efficient
  • Direct comparability through protein expression and phosphorylation level analysis in a single assay
  • Complete analysis from minimal sample volume
  • Coverage of important
    • Signalling pathways
    • Transcription factors
    • Receptor molecules
    • Kinases
  • Individual data analysis options available
    • Protein interaction network analysis
    • Biological and pathway classification 
    • Lists with differential proteins and phosphorylation levels
  • Variety of sample formats possible without the potential bias of a sample fractionation or a sample depletion
  • Results presented in a publication-ready format

Applications

  • T cell activation
  • Tissue treatment response
  • Cell cycle regulation
  • Oxidative stress response
  • Cell adhesion and motility
  • Phosphorylation profiling of various signalling pathways:
    • FAK phosphorylation
    • PI3K-AKT pathway
    • p53 pathway regulation
    • Alzheimer pathway regulation

 

Case study   Target proteins   Contact us

 

Case study

Eight treated cell line samples were compared with 8 control samples. Several proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). While the left plot provides an overview on expression / phosphorylation differences for all tested proteins in the two sample groups, individual levels are depicted in the plots on the right. The sample status is indicated as C = control or T = treated.

 

Reference customers

Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany

Dr. Caroline Pabst. University Hospital Halle, Germany

 

More information

Download our scioPhospho or scioDiscover service brochure.

Information on protein and pathway content / coverage.

Get in touch to answer any remaining questions.

Ask for a quotation to get your study started.

 

Which sample types can be analysed?

With the scioPhospho service a variety of samples can be analysed:

  • fresh frozen tissue samples
  • cellular content
  • cerebrospinal fluid
  • plasma / serum
  • additional sample types on request

 

Sample-to-result service

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • statistical testing for differentially phosphorylated proteins
  • comprehensive study report

 

scioDiscover - Drug Target Discovery

 

You identified a compound with the desired effect ?

 

You are looking for its target(s) ?

Which additional pathways are affected ?

Which proteins are altered in a diseased tissue ?

Get the whole picture of protein expression in multiple pathways !

 

 

 

Profound knowledge about protein expression levels is of utmost importance for the discovery and evaluation of new drug targets. Our Scio-Discover Service is fully immuno-based and covers proteins involved in major disease and signalling pathways as well as secreted proteins. For the highly-parallel protein analysis only minimal amounts of sample are required. No sample fractionation or depletion is needed. Our platform is able to process most biological samples without the need for laborious sample preparation, saving time and resources.

 

Applications:

  • Drug Target Identification and verification studies
  • Mechanistic studies for various indications
  • Correlate Next-Gen-Sequencing Data to protein expression levels
  • Profile cytokine and inflammatory status of your samples

 

Key advantages:

  • Low sample consumption 
  • Investigation of human, murine and rat samples*
  • Fast turnaround time, as low as two weeks**
  • No need for laborious depletion or fractionation of your samples
  • High sensitivity (as sensitive as an ELISA or better)
  • High reproducibility (CV < 10%)
  • Various sample types can be investigated
    • Serum / Plasma
    • Tissue
    • Cell culture
    • Cerebrospinal fluid (CSF)

 

Service Features:

  • Study Layout
  • Protein Extraction and Purification
  • Protein Quantification
  • Protein labelling with high-performance fluorescent dyes
  • Automated array incubation and handling 
  • Array scanning and data acquisition
  • Data analysis according to your requirements
  • Individual study report

 

Overview
Scio-Discover
    Array
Content
    Biomarker
Discovery
    Contact
us

 

 

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

** depending on study design and sample numbers

 

scioUbi case study

DUB knockout cell line versus wild type

  • Direct comparison of protein level changes to ubiquitination changes
  • Easy identification of statistically significant changes
  • Immediate overview of molecular changes

sciomics scioUbi Ubiquitination And Protein Profiling case study
Overview of differentially abundant (x-axis) and ubiquitinated (y-axis) proteins from a cell culture study comparing a DUB knockout group with a wild type sample group.

 

Contact us   Overview scio-Ubi

 

scioUbi

Protein & ubiquitination profiling

scioUbi is a high-content protein expression and ubiquitination level analysis.
With scioUbi you can analyse up to 1,000 proteins in a single assay.

 

Features

  • Screen for differential ubiquitination status
    in >1,000 proteins in parallel in a single assay
  • 4 technical replicates per sample
  • Comprehensive data analysis and presentation including
    an individualised report
  • Customisation of ubiquitin detection on request

Benefits for your research

  • Two in one: High-content protein expression and ubiquitination level analysis in a single assay
  • Proteins selected for biomedical studies: Central regulators (e.g. mTor, p53), transcription factors, receptors
  • Minimal sample (e.g. tissue or cell culture samples) amounts needed for complete analysis
sciomics scioUbi Ubiquitination And Protein Profiling Biological processes selection of proteins
Biological processes
  sciomics scioUbi Ubiquitination And Protein Profiling Cell Cycle proteins coveredCell cycle: proteins in green are covered by the analysis

 

Applications

Biological effects of

  • protein degradation in health and disease
  • protein activity changes
  • protein interaction alterations
  • drug response (e.g. proteasome enhancers)

Others

  • Interplay between ubiquitination and phosphorylation (see scioPhospho)
  • Neurodegenerative diseases
  • Progression of ageing
  • Cell cycle regulation
  • Proteasome activity
  • Transcriptional regulation
  • p53 pathway regulation
  • Alzheimer pathway regulation

 

Contact us

 

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