scioDiscover - Protein Expression Analysis

Protein expression profiling with protein arrays

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 900 proteins are analysed in parallel covered by 1130 antibodies. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples.

 

Features

  • Major pathways, secreted proteins, cell surface and inflammatory markers are covered
  • > 900 analytes
  • > 1,100 antibodies
  • Broad spectrum of indications covered such as oncology and organ failure
  • Robust dual-colour study design
  • Including full data analysis and study report
  • Studies possible in human, murine and rat samples 

 

 Array
 content
    Protein
 profiling
    Biomarker
 Discovery
    Target
 Discovery
    Contact
 us

 

 

Applications

  • Protein expression level analysis
  • Signalling Pathway Profiling
  • Immunsystem activation marker profiling
  • Transcription factor analysis 
  • Cancer pathway screening
  • Cell surface marker profiling
  • Profiling of apoptotic processes/ apoptosis pathway analysis 

 

  • Disease mechanism profiling
  • Pathway activation in stem cells 
  • Analysis of organ damage/failure models 
  • Response of cells and tissue models to oxidative stress analysis
  • Profiling proteomic changes for various cell culture conditions

 

  • Molecular initiate event analysis
  • Compound mode-of-action analysis
  • Prediction markers for toxicological effects 

 

 

  • CD cell surface molecule expression in immune cells  

 

 

 

 

Advantages

  • high-content: 900 proteins analysed with 1130 antibodies
  • broad coverage of:
    • signalling pathway proteins
    • transcription factors
    • apoptosis marker
    • markers for oxidative stress
    • cell surface markers
    • cytokines & chemokines

 

  • minimal sample requirements
  • native matrixes (non-fractionated / non-depleted)
  • sensitivity as ELISA or better

 

  • fully immuno-based assay

 

  • reliable dual-colour study design
  • technical replication ( 4 replicates for each antibody )
  • high reproducibility ( CV < 10% )

 

  • experienced partners with more than ten years of experience in conducting biomedical antibody array studies

 

 

Save time, costs and valuable sample

 

 

 

Reference Customers:

  • Prof. Dr. Faikah Güler, Molecular mechanisms of transplant dysfunction, Medical School Hanover, Hanover, Germany
  • Julian Kamhieh-Milz, Research Center ImmunoSciences, Charité, Berlin, Germany
  • Prof. Jose L. Peiró, Cincinnati Fetal Center Director, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, USA
 

Case studies:

Bladder Cancer

Acute Kidney Injury


 

Which sample types can be analysed ?

With the scioDiscover service a variety of samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request

  

Sample-to-result serviceAntibody microarray workflow

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

 

scioDiscover - Protein Expression Level Profiling

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower cost through a highly-parallel protein expression level analysis using our Scio-Discover Antibody Microarray Platform. Furthermore, you may link to and verify your Genomics and Transcriptomics data with protein expression levels.

 

Applications:

 

Key advantages:

  • High multiplexity (>900 analytes, >1,130 antibodies)
  • Low sample consumption
  • Investigation of human and murine samples*
  • Fast turnaround time, as low as two weeks**
  • No need for laborious depletion or fractionation of your samples
  • High sensitivity (as sensitive as an ELISA or better)
  • High reproducibility (CV < 10%)
  • Various sample types can be investigated
    • Serum / Plasma
    • Tissue
    • Cell culture
    • Cerebrospinal fluid (CSF)

 

Overview
Scio-Discover
    Pathway
Coverage
    Biomarker
Discovery
    Contact
us

 

Reference Customers:

  • Dr. Ghaith Bakdash, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands
  • Dr. Anke Dienelt, Julius Wolff Institut, Charité, Berlin, Germany
  • Dr. Eric Eldering, Academic Medical Center, Amsterdam, The Netherlands
  • Dr. Reece Marillier, Ludwig Institute for Cancer Research Ltd, Brussels, Belgium
  • Prof. Jose L. Peiró, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, USA
 

Case studies:

Bladder Cancer

Acute Kidney Injury

 

* other species on request

** depending on study design and sample numbers

 

scioPhospho: protein + phosphorylation profiling

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation.

 

Features

  • 900 highly relevant proteins are profiled in a single assay
  • Phosphorylation status combined with protein expression levels
  • Tyrosine, serine and threonine phosphorylation
  • Analysis of individual phosphorylation types available
  • "Sample-to-result" service
  • Comprehenisive data analysis and presentation
  • Individualised study report
Protein abundance levels and phosphorylation status for one individual protein out of 900 proteins tested

 

Advantages

  • High-content screening of proteins and phosphorylation is time and ressource efficient
  • Direct comparability through protein expression and phosphorylation level analysis in a single assay
  • Complete analysis from minimal sample volume
  • Coverage of important
    • Signalling pathways
    • Transcription factors
    • Receptor molecules
    • Kinases
  • Individual data analysis options available
    • Protein interaction network analysis
    • Biological and pathway classification 
    • Lists with differential proteins and phosphorylation levels
  • Variety of sample formats possible without the potential bias of a sample fractionation or a sample depletion
  • Results presented in a publication-ready format

 

Applications

  • T cell activation
  • Tissue treatment response
  • Cell cycle regulation
  • Oxidative stress response
  • Cell adhesion and motility
  • Phosphorylation profiling of various signalling pathways:
    • FAK phosphorylation
    • PI3K-AKT pathway
    • p53 pathway regulation
    • Alzheimer pathway regulation

 

 

Case
study
    Target
proteins
    Contact
us

 


 

Case study

Eight treated cell line samples were compared with 8 control samples. Several proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). While the left plot provides an overview on expression / phosphorylation differences for all tested proteins in the two sample groups, individual levels are depicted in the plots on the right. The sample status is indicated as C = control or T = treated.

 

 

Reference customers

  Christopher Y. Park, MD, PhD, Memorial Sloan Kettering Cancer Center, New York, USA

  Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany

  Dr. Caroline Pabst. University Hospital Halle, Germany

 

 

More information

  Download our scioPhospho or scioDiscover service brochure.

  Information on protein and pathway content / coverage.

  Get in touch to answer any remaining questions.

  Ask for a quotation to get your study started.

 

 

Which sample types can be analysed ?

With the scioPhospho service a variety of samples can be analysed:Sample-to-result service

  • fresh frozen tissue samples
  • cellular content
  • cerebrospinal fluid
  • plasma / serum
  • additional sample types on request

 

 

Sample-to-result service

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • statistical testing for differentially phosphorylated proteins
  • comprehensive study report

 

 

 

 

 

 

 

scioCD - Cell surface marker and Cytokine profiling

 

Characterise a certain cell type ?

 

Cell composition in a certain tissue sample ?

 

In-depth profiling of CD-marker expression ?

 

Increase your throughput at high sensitivity levels ?

You aim at high reproducibility ?

 

 

Our scioCD profiling service covers 141 different proteins by 270 different monoclonal antibodies:

All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of tissues or the differentiation status of cultured cells in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently, providing insights in the blood cell composition.

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

 

 

Target
proteins
      Analysis
Service
      Sample
Types
      Product
brochure
      Contact
us

 

 

 

 

Applications

  • profiling of cell differentiation status
  • stem cell differentiation 
  • investigate soluble CD-molecules for e.g. allograft rejection
  • inflammatory response profiling
  • immuno-oncology profiling of check-point inhibitor targets
  • characterisation of tissue-isolated primary cells
  • immune-cell surface marker profiling on
    • various B-cells 
    • stimulated T-cells
    • patient derived T-cells
    • patient derived dendritic cells
  • oncology profiling for new biomarkers e.g. Leukemia
  • tumor micro-environment profiling
  • profile response to stimuli of cell lines
  • evaluate protein expression changes in silencing/overexpression  experiments
 

Advantages

  • high content - 141 proteins are analysed with 270 monoclonal antibodies
  • broad coverage and parallel analysis of
    • CD molecules 
    • cytokines and chemokines
    • other relevant molecules (HLAs, p53)
  • minimal sample requirements
  • native matrixes ( non-fractionated / non-depleted )
  • sensitivity as ELISA or better
  • fully immuno-based assay
  • reliable dual-colour study design
  • high reproducibility, CV < 10%
  • most proteins are covered redundantly by two or more antibodies
  • each antibody is presented in four replicates
  • antibodies are available for follow-up studies

 

 

Target list for the scioCD protein profiling service:

 

CD MarkerGene name CD MarkerGene name  Chemokine / CytokineGene name  Additional proteinsGene name
CD1aLEU6 CD50ICAM3  GM-CSFCSF2  ANCAPRTN3
CD2SRBC CD52HE5  IFN alphaIFNA1  BDNFBDNF
CD3T3G CD53TSPAN25  IFN gammaIFNG  Cox1PTGS1
CD4LEU3 CD54ICAM1  IL-1alphaIL1A  CYTLCYTL1
CD5LEU1 CD55DAF  IL-1betaIL1B  IgEFCER1A
CD6TP120 CD56NCAM1  IL-2IL2  HLA I 
CD7LEU9 CD57B3GAT1  IL-4IL4  HLA-ABC 
CD8MAL CD58LFA3  IL-6IL6  HLA-DPHLA-DPB1
CD9MIC3 CD59MIC11  IL-7IL7  HLA-DQ 
CD10MME CD61ITGB3  IL-8CXCL8  HLA-DR 
CD11aITGAL CD62LSELL  IL-10IL10  MPOMPO
CD11bITGAM CD62pSELP  IL-12BIL12B  NT-4NTF4
CD11cITGAX CD63TSPAN30  IL-13IL13  NTALLAT2
CD13ANPEP CD66aCEACAM1  IL-15IL15  pan HLA-class II 
CD14  CD66bCEACAM8  IL-16IL16  p53TP53
CD15FUT4 CD66cCEACAM6  IL-18IL18  p72SykSYK
CD16FCGR3A CD66dCEACAM3  IL-37IL37  TRYG1TPSG1
CD17  CD66eCEACAM5  LIFLIF  TSLPRCRLF2
CD18ITGB2 CD69CLEC2C  TNF alphaTNF  tTGTGM2
CD19LEU12 CD70TNFSF7  TSLPTSLP  VEGFVEGFA
CD20MS4A1 CD71TFRC        
CD21CR2 CD72Lyb-2  Eotaxin-1CCL11    
CD22SIGLEC2 CD79aMB1  MIP-1 alphaCCL3    
CD23FCER2 CD80LAB7  RANTESCCL5    
CD24  CD86LAB72  MCP-2CCL8    
CD25IL2RA CD95FAS  MCP-3CCL7    
CD27TNFRSF7 CD97   MIP-4CCL18    
CD28TP44 CD98SLC3A2        
CD29ITGB1 CD99MIC2        
CD30TNFRSF8 CD105ENG        
CD31PECAM1 CD106VCAM1        
CD33SIGLEC3 CD116CSF2RA        
CD34  CD117KIT        
CD35CR1 CD123IL3RA        
CD36GP3B CD131CSF2RB        
CD37TSPAN26 CD137TNFRSF9        
CD38ADPRC 1 CD139         
CD40TNFRSF5 CD147BSG        
CD41ITGA2B CD162SELPLG        
CD42bGP1BA CD177NB1        
CD43SPN CD222IGF2R        
CD44MDU2 CD223LAG3        
CD45PTPRC CD230PRNP        
CD46MCP CD235aGYPA        
CD47MER6 CD235bGYPB        
CD48BCM1 CD253TNFSF10        
CD49dITGA4 CD274PDL1        
   CD279PD1        

 

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request

  

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

 

scioDiscover - Protein Biomarker Discovery

Personalised Medicine and Diagnostic Research require techniques to discover and verify novel and more accurate Biomarkers. We offer a comprehensive immuno-based Protein Biomarker Discovery Service using complex Antibody Microarrays. This platform covers key pathway proteins for various diseases as well as secreted proteins and was successfully applied in a variety of biomedical studies. The potential of our platform is underlined by the successful discovery of 4 innovative biomarker signatures.

Due to the fully immuno-based workflow a major platform change is not required for further development and therefore can decrease attrition rates of biomarker candidates. With more than 10 years of experience Sciomics is the ideal partner for Protein Biomarker Discovery and Verification. 

 

 

Biomarker Discovery for:

  • Diagnosis
  • Differential Diagnosis
  • Prognosis
  • Therapy Stratification / Drug Response Prediction
  • Companion Diagnostics

 

Features:

  • Design of appropriate study layout
  • Protein Extraction & Purification
  • Protein Quantification
  • Protein Labelling with high performance fluorescent dyes
  • Fully-automated array incubation and handling 
  • Array scanning, data acquisition and spot recognition
  • Data analysis according to your requirements
  • Individual study report

 

Key advantages:

  • Low sample consumption 
  • Investigation of human and murine samples*
  • Fast turnaround time, as low as two weeks**
  • No need for laborious depletion or fractionation of your samples
  • High sensitivity (as sensitive as an ELISA or better)
  • High reproducibility (CV < 10%)
  • Various sample types can be investigated
    • Serum / Plasma
    • Tissue
    • Cell culture
    • Cerebrospinal fluid (CSF)
  • Verification and clinical validation without major platform change
  • Parallel Verification of multiple Biomarker Candidates with Custom Antibody Arrays

 

Overview
Scio-Discover
    Array
Content
    Quality
Assurance
    Contact
us

 

 

Reference Customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

 

Case studies:

Bladder Cancer

Acute Kidney Injury

 

 

* other species on request

** depending on study design and sample numbers

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