scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation.
Eight treated cell line samples were compared with 8 control samples. Several proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). While the left plot provides an overview on expression / phosphorylation differences for all tested proteins in the two sample groups, individual levels are depicted in the plots on the right. The sample status is indicated as C = control or T = treated.
Christopher Y. Park, MD, PhD, Memorial Sloan Kettering Cancer Center, New York, USA
Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany
Dr. Caroline Pabst. University Hospital Halle, Germany
Information on protein and pathway content / coverage.
With the scioPhospho service a variety of samples can be analysed:
- fresh frozen tissue samples
- cellular content
- cerebrospinal fluid
- plasma / serum
- additional sample types on request
Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.
Our analysis service includes:
- definition of an appropriate study design
- sample preparation
- protein extraction
- protein concentration measurements
- protein quality control
- sample labelling
- sample purification
- incubation of the samples on antibody microarrays
- microarray scanning
- raw data acquisition
- data normalisation
- data analysis including cluster analysis
- statistical testing for differentially abundant proteins
- statistical testing for differentially phosphorylated proteins
- comprehensive study report