Target proteins

Our scioDiscover and scioPhospho services are based on complex Antibody Microarrays, also called analytical Protein Arrays or Protein Capture Arrays. Within a single experiment 900 proteins can be profiled in parallel requiring only minimal amounts of sample. The targets proteins were carefully selected over a period of more than 15 years to cover a broad set of different protein classes as well as pathways important in biomedical studies.

Thereby, the protein profiling covers key pathways in various diseases such as cancer, neurological disorders as well as organ failure. Given the wide spectrum of applications and indications the service is ideal for high-content protein expression level analysis from various biological samples. The broad coverage of many important pathways, extracellular and membrane proteins as well as transcription factors, renders scioDiscover a versatile and efficient tool for discovery projects.

Please find below the distribution of the proteins targeted by scioDiscover according their biological processes (Fig.1), their protein classes (Fig.2), their molecular function (Fig.3) as well as their cellular compartment (Fig.4). In addition, pathways covered by scioDiscover are listed in Table 1.

For the pathways TNF signaling, Cell cycle regulation and pathways important in Cancer, Tuberculosis and Huntington's Disease covered targets are depicted in green.

Please contact us for more information regarding targets and pathways covered or to discuss how we can support your study with a highly-parallel protein analysis.

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

Overview
Scio-Discover
    Biomarker
Discovery
    Target
Discovery
    Contact
us

 

 

Classification of target proteins

Biological processes

Protein classes

biological processes covered by protein array analysis

protein classes covered by protein array analysis

Fig. 1: Distribution of target proteins within different biological processes. The majority of the antibodies on the microarray detect proteins involved in cellular or metabolic processes or are involved biological regulation, in a developmental or immune system process or as a response to a stimulus.

Fig. 2: Distribution of target proteins within different protein classes. With scioDiscover 122 signaling molecules, 113 receptors, 109 nucleic acid binding proteins and 71 transcription factors can be analysed in parallel.

Molecular functions

Cellular compartmenrt

molecular functions covered by protein array analysis

 

cellular compartments covered by protein array analysis

 

Fig. 3: Distribution of target proteins within different molecular functions. The majority of proteins on the protein microarrays measures proteins involved in binding or with a catalytic or receptor activity.

Fig. 4: Target proteins classified according their Cellular Compartment.

 
 

Coverage of pathways

Selected Pathwaysno. targets
Inflammation mediated by chemokine and cytokine signaling (P00031)40
Apoptosis signaling (P00006)38
Gonadotropin releasing hormone receptor (P06664)35
CCKR signaling map (P06959)35
Integrin signalling (P00034)29
Angiogenesis (P00005)28
Interleukin signaling (P00036)27
Huntington disease (P00029)26
p53 pathway (P00059)22
T cell activation (P00053)22
B cell activation (P00010)21
Ras Pathway (P04393)18
Wnt signaling (P00057)17
TGF-beta signaling (P00052)17
EGF receptor signaling (P00018)17
Alzheimer disease-presenilin (P00004)15
FAS signaling (P00020)15
FGF signaling (P00021)14
VEGF signaling (P00056)13
Toll receptor signaling (P00054)13
Parkinson disease (P00049)12
PDGF signaling (P00047)12
Insulin / IGF pathway-mitogen activated protein kinase kinase / MAP kinase cascade (P00032)12
Alzheimer disease - amyloid secretase  (P00003)11
p53 pathway feedback loops 2 (P04398)10
Endothelin signaling (P00019)10
Blood coagulation (P00011)10
Plasminogen activating cascade (P00050)9
Cytoskeletal regulation by Rho GTPase (P00016)9
Interferon-gamma signaling (P00035)8
Insulin/IGF pathway-protein kinase B signaling cascade (P00033)8
Ubiquitin proteasome (P00060)7
Transcription regulation by bZIP transcription factor (P00055)7
PI3 kinase (P00048)7
Oxidative stress response (P00046)7
Cadherin signaling (P00012)7
 

 

Highly parallel protein analysis of TNF Signaling

Fig. 5: Proteins in the TNF signalling pathway covered by our protein array are shown in green.

 

 Parallel Analysis of Cell Cycle Proteins by complex antibody arrays

Fig. 6: Cell Cycle Proteins covered by antibody array are depicted in green.

 

 Cancer protein profiling

Fig 7: scioDiscover has a broad coverage of diverse pathways involved in cancer. Proteins in green can be profiled with scioDiscover antibody arrays.

 

 Protein analysis in Huntington's disease

Fig. 8: scioDiscover has a good coverage of proteins involved in various diseases such as Huntington's disease. Proteins analysed in parallel by scioDiscover protein arrays are shown in green

 

Proteins involved in Tuberculosis

Fig. 9: Pathways involved in Tuberculosis are shown. Proteins covered by the scioDiscover service are shown in green.

 

 

Do you like to subscribe our newsletter ?      Yes   No

Products & Services

scioDiscover - Protein Expression Analysis

scioDiscover - Protein Expression Analysis

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker…
Read more
scioDiscover - Protein Expression Level Profiling

scioDiscover - Protein Expression Level Profiling

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower…
Read more
scioPhospho: protein + phosphorylation profiling

scioPhospho: protein + phosphorylation profiling

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway…
scioCD - Cell surface marker and Cytokine profiling

scioCD - Cell surface marker and Cytokine profiling

Characterise a certain cell type ? Cell composition in a certain tissue sample ? In-depth profiling of CD-marker expression ? Increase your throughput at high sensitivity levels ? You aim…
scioDiscover - Protein Biomarker Discovery

scioDiscover - Protein Biomarker Discovery

Personalised Medicine and Diagnostic Research require techniques to discover and verify novel and more accurate Biomarkers. We offer a comprehensive immuno-based Protein Biomarker Discovery Service using complex Antibody Microarrays. This…
scioSpec - Antibody Specificity Assessment

scioSpec - Antibody Specificity Assessment

Be sure that your antibody is fit for your application. Profile your therapeutic or diagnostic antibody against more than 9000 human proteins. Have a comprehensive cross-reactivity and off-target reactivity profile…
scioExtract - Protein Extraction Buffers

scioExtract - Protein Extraction Buffers

The Scio-Extract Protein Extraction Buffers are optimised for superior protein isolation performance from all cell compartments and of membrane proteins. Their non-denaturing properties enable the extraction of active proteins under…
scioSpot - Custom Antibody Microarrays

scioSpot - Custom Antibody Microarrays

Antibody Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody chips as well as arrays of…
scioSpot - Microarray Production

scioSpot - Microarray Production

Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody-, protein-, peptide- or DNA/RNA chips. Benefit from…

News

New article | Expansion of a BDCA1+CD14+ Myeloid Cell Population in Melanoma Patients May Attenuate the Efficacy of Dendritic Cell Vaccines.

| July 2016 | One of our customer projects was published in Cancer Research. In this study Bakdash et al. analysed the expansion of a BDCA1+CD14+ myeloid cell population in the context of immune therapy of melanoma. Within this study a protein profiling of cellular patient samples was performed with scioCD antibody arrays.

Interview | Advanced Biomarkers for Precision Medicine

New article | Analysis of the blood protein profile in B-cell lymphoma patients

| 12.12.2013 | There is a new article in the current issue of Proteomics Clinical Applications on an analysis of the blood protein profile of different B-cell lymphoma using complex antibody microarrays. 200 blood samples from patients with Diffuse Large B-cell Lymphoma (DLBCL), Follicular Lymphoma (FL), Chronic Lymphocytic Leukemia (CLL) as well as healthy controls were analysed and compared with each other. The study identified proteins with discriminating power among the different lymphoma types, as well as subgroups within each lymphoma entity.

New article | Prediction of recurrence in bladder cancer

| 08.05.2014 | In a study published in Proteomics we identified proteins with differential abundance in bladder tumours with and without a local recurrence using antibody array technology. Next to interesting differences of the two tumour subtypes from a scientific point of view, we found a protein biomarker signature with high potential for a prognosis. This could enabling urologists in future to adjust the therapy and surveillance scheme accordingly. Further validation studies are ongoing. →Case study report

Tweets

  • yesterday

    RNA has a very short life time of only 2 minutes - look at proteins to avoid experimental bias - scioDiscover -… https://t.co/M6wFNYaDrE

  • 2 days ago

    Precision Medicine - from proteomic discovery to clinical application. Join our webinar on 30th October, 3:30pm CET: https://t.co/fWiJZdFyOC

  • 9 days ago

    Precision Medicine - from proteomic discovery to clinical application. Join our webinar on 30th October, 3:30pm CET: https://t.co/vzzSqhb1pD

  • 31 days ago

    "We absolutely recommend Sciomics to everyone interested in an antibody array service" -from Prof Gräler -Thank you! https://t.co/RJvdhMZxRa

  • 38 days ago

    Looking forward to the webinar presenting our platform for protein #biomarker identification and verification from… https://t.co/tDgtQvrDKp