scioCD - Cell surface marker and Cytokine profiling

 

Characterise a certain cell type ?

 

Cell composition in a certain tissue sample ?

 

In-depth profiling of CD-marker expression ?

 

Increase your throughput at high sensitivity levels ?

You aim at high reproducibility ?

 

 

Our scioCD profiling service covers 141 different proteins by 270 different monoclonal antibodies:

All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of tissues or the differentiation status of cultured cells in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently, providing insights in the blood cell composition.

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

 

 

Target
proteins
      Analysis
Service
      Sample
Types
      Product
brochure
      Contact
us

 

 

 

 

Applications

  • profiling of cell differentiation status
  • stem cell differentiation 
  • investigate soluble CD-molecules for e.g. allograft rejection
  • inflammatory response profiling
  • immuno-oncology profiling of check-point inhibitor targets
  • characterisation of tissue-isolated primary cells
  • immune-cell surface marker profiling on
    • various B-cells 
    • stimulated T-cells
    • patient derived T-cells
    • patient derived dendritic cells
  • oncology profiling for new biomarkers e.g. Leukemia
  • tumor micro-environment profiling
  • profile response to stimuli of cell lines
  • evaluate protein expression changes in silencing/overexpression  experiments
 

Advantages

  • high content - 141 proteins are analysed with 270 monoclonal antibodies
  • broad coverage and parallel analysis of
    • CD molecules 
    • cytokines and chemokines
    • other relevant molecules (HLAs, p53)
  • minimal sample requirements
  • native matrixes ( non-fractionated / non-depleted )
  • sensitivity as ELISA or better
  • fully immuno-based assay
  • reliable dual-colour study design
  • high reproducibility, CV < 10%
  • most proteins are covered redundantly by two or more antibodies
  • each antibody is presented in four replicates
  • antibodies are available for follow-up studies

 

 

Target list for the scioCD protein profiling service:

 

CD MarkerGene name CD MarkerGene name  Chemokine / CytokineGene name  Additional proteinsGene name
CD1aLEU6 CD50ICAM3  GM-CSFCSF2  ANCAPRTN3
CD2SRBC CD52HE5  IFN alphaIFNA1  BDNFBDNF
CD3T3G CD53TSPAN25  IFN gammaIFNG  Cox1PTGS1
CD4LEU3 CD54ICAM1  IL-1alphaIL1A  CYTLCYTL1
CD5LEU1 CD55DAF  IL-1betaIL1B  IgEFCER1A
CD6TP120 CD56NCAM1  IL-2IL2  HLA I 
CD7LEU9 CD57B3GAT1  IL-4IL4  HLA-ABC 
CD8MAL CD58LFA3  IL-6IL6  HLA-DPHLA-DPB1
CD9MIC3 CD59MIC11  IL-7IL7  HLA-DQ 
CD10MME CD61ITGB3  IL-8CXCL8  HLA-DR 
CD11aITGAL CD62LSELL  IL-10IL10  MPOMPO
CD11bITGAM CD62pSELP  IL-12BIL12B  NT-4NTF4
CD11cITGAX CD63TSPAN30  IL-13IL13  NTALLAT2
CD13ANPEP CD66aCEACAM1  IL-15IL15  pan HLA-class II 
CD14  CD66bCEACAM8  IL-16IL16  p53TP53
CD15FUT4 CD66cCEACAM6  IL-18IL18  p72SykSYK
CD16FCGR3A CD66dCEACAM3  IL-37IL37  TRYG1TPSG1
CD17  CD66eCEACAM5  LIFLIF  TSLPRCRLF2
CD18ITGB2 CD69CLEC2C  TNF alphaTNF  tTGTGM2
CD19LEU12 CD70TNFSF7  TSLPTSLP  VEGFVEGFA
CD20MS4A1 CD71TFRC        
CD21CR2 CD72Lyb-2  Eotaxin-1CCL11    
CD22SIGLEC2 CD79aMB1  MIP-1 alphaCCL3    
CD23FCER2 CD80LAB7  RANTESCCL5    
CD24  CD86LAB72  MCP-2CCL8    
CD25IL2RA CD95FAS  MCP-3CCL7    
CD27TNFRSF7 CD97   MIP-4CCL18    
CD28TP44 CD98SLC3A2        
CD29ITGB1 CD99MIC2        
CD30TNFRSF8 CD105ENG        
CD31PECAM1 CD106VCAM1        
CD33SIGLEC3 CD116CSF2RA        
CD34  CD117KIT        
CD35CR1 CD123IL3RA        
CD36GP3B CD131CSF2RB        
CD37TSPAN26 CD137TNFRSF9        
CD38ADPRC 1 CD139         
CD40TNFRSF5 CD147BSG        
CD41ITGA2B CD162SELPLG        
CD42bGP1BA CD177NB1        
CD43SPN CD222IGF2R        
CD44MDU2 CD223LAG3        
CD45PTPRC CD230PRNP        
CD46MCP CD235aGYPA        
CD47MER6 CD235bGYPB        
CD48BCM1 CD253TNFSF10        
CD49dITGA4 CD274PDL1        
   CD279PD1        

 

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request

  

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

 

 

Do you like to subscribe our newsletter ?      Yes   No

Products & Services

scioDiscover - Protein Expression Analysis

scioDiscover - Protein Expression Analysis

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker…
Read more
scioDiscover - Protein Expression Level Profiling

scioDiscover - Protein Expression Level Profiling

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower…
Read more
scioPhospho: protein + phosphorylation profiling

scioPhospho: protein + phosphorylation profiling

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway…
scioCD - Cell surface marker and Cytokine profiling

scioCD - Cell surface marker and Cytokine profiling

Characterise a certain cell type ? Cell composition in a certain tissue sample ? In-depth profiling of CD-marker expression ? Increase your throughput at high sensitivity levels ? You aim…
scioDiscover - Protein Biomarker Discovery

scioDiscover - Protein Biomarker Discovery

Personalised Medicine and Diagnostic Research require techniques to discover and verify novel and more accurate Biomarkers. We offer a comprehensive immuno-based Protein Biomarker Discovery Service using complex Antibody Microarrays. This…
scioSpec - Antibody Specificity Assessment

scioSpec - Antibody Specificity Assessment

Be sure that your antibody is fit for your application. Profile your therapeutic or diagnostic antibody against more than 9000 human proteins. Have a comprehensive cross-reactivity and off-target reactivity profile…
scioExtract - Protein Extraction Buffers

scioExtract - Protein Extraction Buffers

The Scio-Extract Protein Extraction Buffers are optimised for superior protein isolation performance from all cell compartments and of membrane proteins. Their non-denaturing properties enable the extraction of active proteins under…
scioSpot - Custom Antibody Microarrays

scioSpot - Custom Antibody Microarrays

Antibody Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody chips as well as arrays of…
scioSpot - Microarray Production

scioSpot - Microarray Production

Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody-, protein-, peptide- or DNA/RNA chips. Benefit from…

News

New article | Expansion of a BDCA1+CD14+ Myeloid Cell Population in Melanoma Patients May Attenuate the Efficacy of Dendritic Cell Vaccines.

| July 2016 | One of our customer projects was published in Cancer Research. In this study Bakdash et al. analysed the expansion of a BDCA1+CD14+ myeloid cell population in the context of immune therapy of melanoma. Within this study a protein profiling of cellular patient samples was performed with scioCD antibody arrays.

Interview | Advanced Biomarkers for Precision Medicine

New article | Analysis of the blood protein profile in B-cell lymphoma patients

| 12.12.2013 | There is a new article in the current issue of Proteomics Clinical Applications on an analysis of the blood protein profile of different B-cell lymphoma using complex antibody microarrays. 200 blood samples from patients with Diffuse Large B-cell Lymphoma (DLBCL), Follicular Lymphoma (FL), Chronic Lymphocytic Leukemia (CLL) as well as healthy controls were analysed and compared with each other. The study identified proteins with discriminating power among the different lymphoma types, as well as subgroups within each lymphoma entity.

New article | Prediction of recurrence in bladder cancer

| 08.05.2014 | In a study published in Proteomics we identified proteins with differential abundance in bladder tumours with and without a local recurrence using antibody array technology. Next to interesting differences of the two tumour subtypes from a scientific point of view, we found a protein biomarker signature with high potential for a prognosis. This could enabling urologists in future to adjust the therapy and surveillance scheme accordingly. Further validation studies are ongoing. →Case study report

Tweets

  • yesterday

    RNA has a very short life time of only 2 minutes - look at proteins to avoid experimental bias - scioDiscover -… https://t.co/M6wFNYaDrE

  • 2 days ago

    Precision Medicine - from proteomic discovery to clinical application. Join our webinar on 30th October, 3:30pm CET: https://t.co/fWiJZdFyOC

  • 9 days ago

    Precision Medicine - from proteomic discovery to clinical application. Join our webinar on 30th October, 3:30pm CET: https://t.co/vzzSqhb1pD

  • 31 days ago

    "We absolutely recommend Sciomics to everyone interested in an antibody array service" -from Prof Gräler -Thank you! https://t.co/RJvdhMZxRa

  • 38 days ago

    Looking forward to the webinar presenting our platform for protein #biomarker identification and verification from… https://t.co/tDgtQvrDKp