scioCD - Cell surface marker and Cytokine profiling

 

Characterise a certain cell type ?

 

Cell composition in a certain tissue sample ?

 

In-depth profiling of CD-marker expression ?

 

Increase your throughput at high sensitivity levels ?

You aim at high reproducibility ?

 

 

Our scioCD profiling service covers 141 different proteins by 270 different monoclonal antibodies:

All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of tissues or the differentiation status of cultured cells in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently, providing insights in the blood cell composition.

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

 

 

Target
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Applications

  • profiling of cell differentiation status
  • stem cell differentiation 
  • investigate soluble CD-molecules for e.g. allograft rejection
  • inflammatory response profiling
  • immuno-oncology profiling of check-point inhibitor targets
  • characterisation of tissue-isolated primary cells
  • immune-cell surface marker profiling on
    • various B-cells 
    • stimulated T-cells
    • patient derived T-cells
    • patient derived dendritic cells
  • oncology profiling for new biomarkers e.g. Leukemia
  • tumor micro-environment profiling
  • profile response to stimuli of cell lines
  • evaluate protein expression changes in silencing/overexpression  experiments
 

Advantages

  • high content - 141 proteins are analysed with 270 monoclonal antibodies
  • broad coverage and parallel analysis of
    • CD molecules 
    • cytokines and chemokines
    • other relevant molecules (HLAs, p53)
  • minimal sample requirements
  • native matrixes ( non-fractionated / non-depleted )
  • sensitivity as ELISA or better
  • fully immuno-based assay
  • reliable dual-colour study design
  • high reproducibility, CV < 10%
  • most proteins are covered redundantly by two or more antibodies
  • each antibody is presented in four replicates
  • antibodies are available for follow-up studies

 

 

Target list for the scioCD protein profiling service:

 

CD MarkerGene name CD MarkerGene name  Chemokine / CytokineGene name  Additional proteinsGene name
CD1aLEU6 CD50ICAM3  GM-CSFCSF2  ANCAPRTN3
CD2SRBC CD52HE5  IFN alphaIFNA1  BDNFBDNF
CD3T3G CD53TSPAN25  IFN gammaIFNG  Cox1PTGS1
CD4LEU3 CD54ICAM1  IL-1alphaIL1A  CYTLCYTL1
CD5LEU1 CD55DAF  IL-1betaIL1B  IgEFCER1A
CD6TP120 CD56NCAM1  IL-2IL2  HLA I 
CD7LEU9 CD57B3GAT1  IL-4IL4  HLA-ABC 
CD8MAL CD58LFA3  IL-6IL6  HLA-DPHLA-DPB1
CD9MIC3 CD59MIC11  IL-7IL7  HLA-DQ 
CD10MME CD61ITGB3  IL-8CXCL8  HLA-DR 
CD11aITGAL CD62LSELL  IL-10IL10  MPOMPO
CD11bITGAM CD62pSELP  IL-12BIL12B  NT-4NTF4
CD11cITGAX CD63TSPAN30  IL-13IL13  NTALLAT2
CD13ANPEP CD66aCEACAM1  IL-15IL15  pan HLA-class II 
CD14  CD66bCEACAM8  IL-16IL16  p53TP53
CD15FUT4 CD66cCEACAM6  IL-18IL18  p72SykSYK
CD16FCGR3A CD66dCEACAM3  IL-37IL37  TRYG1TPSG1
CD17  CD66eCEACAM5  LIFLIF  TSLPRCRLF2
CD18ITGB2 CD69CLEC2C  TNF alphaTNF  tTGTGM2
CD19LEU12 CD70TNFSF7  TSLPTSLP  VEGFVEGFA
CD20MS4A1 CD71TFRC        
CD21CR2 CD72Lyb-2  Eotaxin-1CCL11    
CD22SIGLEC2 CD79aMB1  MIP-1 alphaCCL3    
CD23FCER2 CD80LAB7  RANTESCCL5    
CD24  CD86LAB72  MCP-2CCL8    
CD25IL2RA CD95FAS  MCP-3CCL7    
CD27TNFRSF7 CD97   MIP-4CCL18    
CD28TP44 CD98SLC3A2        
CD29ITGB1 CD99MIC2        
CD30TNFRSF8 CD105ENG        
CD31PECAM1 CD106VCAM1        
CD33SIGLEC3 CD116CSF2RA        
CD34  CD117KIT        
CD35CR1 CD123IL3RA        
CD36GP3B CD131CSF2RB        
CD37TSPAN26 CD137TNFRSF9        
CD38ADPRC 1 CD139         
CD40TNFRSF5 CD147BSG        
CD41ITGA2B CD162SELPLG        
CD42bGP1BA CD177NB1        
CD43SPN CD222IGF2R        
CD44MDU2 CD223LAG3        
CD45PTPRC CD230PRNP        
CD46MCP CD235aGYPA        
CD47MER6 CD235bGYPB        
CD48BCM1 CD253TNFSF10        
CD49dITGA4 CD274PDL1        
   CD279PD1        

 

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request

  

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

 

 

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News

Meeting | EACR25

| June 30 - July 3 2018 | Meet us at the 25th Biennial Congress of the European Association for Cancer Research in Amsterdam. Please visit our booth 22 or get in touch for a scheduled appointment to learn more about our protein profiling services.

New article | Role of Corneal Stromal Cells on Epithelial Cell Function during Wound Healing

| February 2018 | Researchers at Rostock University Medical Center published an article on the role of Corneal Stromal Cells on Epithelial Cell Function during Wound Healing in the International Journal of Molecular Sciences.

> Customer Testimonial Bhavani Kowtharapu:

"I am really happy with service of Sciomics. Very friendly team and really fast service and we have the results in our hand including bioinformatics analysis within 2 weeks. Thank you Sciomics team."

> More testimonials

Research | Participation in collaborative clinical studies for the treatment of MCDS

| January 2018 | The multinational consortium MCDS-therapy has been granted 5.7 Million Euro by the European Commission for the development of novel therapeutic approaches in rare skeletal disorders. Sciomics' expertise with the antibody microarray technology herein serves as a valuable tool for biomarker identification and personalized treatment monitoring. > Press release

New article | Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells

| January 2018 | A scioDiscover protein microarray study contributed to an article in Cell Reports. In this study Gerard Ruiz-Babot et al. analysed a donor specific reprogramming of cells.

Customer testimonial Leonardo Guasti, PhD (senior author):

This has undoubtedly opened new avenues in our research. We have found the Sciomics team to be extremely helpful and efficient during the whole process, from advising the best strategy to the final interpretation of results.

> More testimonials

A glas full of hope | Ein Glas Hoffnung

| January 2018 | Article on the StartUp Sciomics and our antibody microarray platform by our partner SCHOTT nexterion.

> English version "A glass full of hope"

> Deutsche Version "Ein Glas Hoffnung"

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