scioSpot - Microarray Production

Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody-, protein-, peptide- or DNA/RNA chips. Benefit from excellent quality manufacturing and a wide choice of microarray support surfaces and coupling-chemistry optimised for your application.

 

Microarrays possible:

 

Key Applications:

  • SNP analysis and genomic biomarkers (DNA arrays)
  • autoantibody profiling (protein and peptide arrays)
  • protein biomarker verification (antibody arrays)
  • protein expression level analysis and in-depth pathway profiling (antibody arrays)
  • highly-parallel affinity molecule characterisation (various array types)

 

Custom modifications available:

  • Surface chemistry
  • Optimised coupling-chemistry for different molecule classes
  • Spot size / diameter
  • Spot density
  • Numbers of replicates
  • Spotting layout
  • Number of subarrays (distinct incubation areas per microarray)

 

Reference customers:

Hutman Diagnostics, Switzerland

Dr. Stéphanie Renaud, University of Lausanne, Switzerland

 

This email address is being protected from spambots. You need JavaScript enabled to view it. for further information and to discuss your custom microarray production.

 

Protein Profiling Platform - Quality Assurance

High quality standards are essential for robust and reliable Biomarker Discovery and Verification as well as for the discovery of new Drug Targets. During the last 10 years a stringent quality assurance workflow has been established for our Antibody Array Platform in order to conduct immuno-based biomedical discovery studies with excellent standards in terms of reproducibility and platform stability. The automated assay workflow further improves the performance of our service. 

 

Biomarker Discovery Phase - scioDiscover Platform

  • Stringent antigen design for maximised specificity of the resulting antibodies
  • Antibodies are antigen affinity-purified
  • Targets identified through transcriptional studies and expert input
  • Stringent QC management
  • Features:  
    • cross-species activity (human and mouse for high versatility)
    • detection of several protein isoforms
    • cost-efficiency through high content

  

Verification Phase - Custom Array Platform

  • Stringent antigen design for maximised specificity of the resulting antibodies
  • Antibodies are antigen affinity-purified
  • In-house QC on Western Blot (different cell lines or tissues) and optional ELISA-based tests
  • In-house QC on large-scale protein arrays (> 9.000 human proteins)
  • In-depth validation of array performance
  • Definition of biomarker signature with highest accuracy
  • Selection of appropriate binders / sandwich pairs
  • Features:  
    • specifically designed antibodies for further use in diagnostic assays
    • fully renewable antibody formats
    • in-depth quality controlled (WB, ELISA)
    • comprehensive cross-reactivity profile (large-scale protein arrays)

 

Orthogonal Biomarker Validation

Verified Candidates can be further validated by orthogonal methods according to customer’s requirements

  • Western Blotting ( WB )
  • ELISA
  • Immuno-Precipitation ( IP )
  • Mass spectrometry ( MS )
  • Biacore

 

Overview
Scio-Discover
    Biomarker
Discovery
    Custom Microarrays
for Biomarker Verification
    Contact
us

Target proteins

Our scioDiscover and scioPhospho services are based on complex Antibody Microarrays, also called analytical Protein Arrays or Protein Capture Arrays. Within a single experiment 1030 proteins can be profiled in parallel requiring only minimal amounts of sample. The targets proteins were carefully selected over a period of more than 15 years to cover a broad set of different protein classes as well as pathways important in biomedical studies.

Thereby, the protein profiling covers key pathways in various diseases such as cancer, neurological disorders as well as organ failure. Given the wide spectrum of applications and indications the service is ideal for high-content protein expression level analysis from various biological samples. The broad coverage of many important pathways, extracellular and membrane proteins as well as transcription factors, renders scioDiscover a versatile and efficient tool for discovery projects.

Please find below the distribution of the proteins targeted by scioDiscover according their biological processes (Fig.1), their protein classes (Fig.2), their molecular function (Fig.3) as well as their cellular compartment (Fig.4). In addition, pathways covered by scioDiscover are listed in Table 1.

For the pathways TNF signaling, Cell cycle regulation and pathways important in Cancer, Tuberculosis and Huntington's Disease covered targets are depicted in green.

Please contact us for more information regarding targets and pathways covered or to discuss how we can support your study with a highly-parallel protein analysis.

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

Overview
Scio-Discover
    Biomarker
Discovery
    Target
Discovery
    Contact
us

 

 

Classification of target proteins

Biological processes

Protein classes

biological processes covered by protein array analysis

protein classes covered by protein array analysis

Fig. 1: Distribution of target proteins within different biological processes. The majority of the antibodies on the microarray detect proteins involved in cellular or metabolic processes or are involved biological regulation, in a developmental or immune system process or as a response to a stimulus.

Fig. 2: Distribution of target proteins within different protein classes. With scioDiscover 122 signaling molecules, 113 receptors, 109 nucleic acid binding proteins and 71 transcription factors can be analysed in parallel.

Molecular functions

Cellular compartmenrt

molecular functions covered by protein array analysis

 

cellular compartments covered by protein array analysis

 

Fig. 3: Distribution of target proteins within different molecular functions. The majority of proteins on the protein microarrays measures proteins involved in binding or with a catalytic or receptor activity.

Fig. 4: Target proteins classified according their Cellular Compartment.

 
 

Coverage of pathways

Selected Pathwaysno. targets
Inflammation mediated by chemokine and cytokine signaling (P00031)40
Apoptosis signaling (P00006)38
Gonadotropin releasing hormone receptor (P06664)35
CCKR signaling map (P06959)35
Integrin signalling (P00034)29
Angiogenesis (P00005)28
Interleukin signaling (P00036)27
Huntington disease (P00029)26
p53 pathway (P00059)22
T cell activation (P00053)22
B cell activation (P00010)21
Ras Pathway (P04393)18
Wnt signaling (P00057)17
TGF-beta signaling (P00052)17
EGF receptor signaling (P00018)17
Alzheimer disease-presenilin (P00004)15
FAS signaling (P00020)15
FGF signaling (P00021)14
VEGF signaling (P00056)13
Toll receptor signaling (P00054)13
Parkinson disease (P00049)12
PDGF signaling (P00047)12
Insulin / IGF pathway-mitogen activated protein kinase kinase / MAP kinase cascade (P00032)12
Alzheimer disease - amyloid secretase  (P00003)11
p53 pathway feedback loops 2 (P04398)10
Endothelin signaling (P00019)10
Blood coagulation (P00011)10
Plasminogen activating cascade (P00050)9
Cytoskeletal regulation by Rho GTPase (P00016)9
Interferon-gamma signaling (P00035)8
Insulin/IGF pathway-protein kinase B signaling cascade (P00033)8
Ubiquitin proteasome (P00060)7
Transcription regulation by bZIP transcription factor (P00055)7
PI3 kinase (P00048)7
Oxidative stress response (P00046)7
Cadherin signaling (P00012)7
 

 

Highly parallel protein analysis of TNF Signaling

Fig. 5: Proteins in the TNF signalling pathway covered by our protein array are shown in green.

 

 Parallel Analysis of Cell Cycle Proteins by complex antibody arrays

Fig. 6: Cell Cycle Proteins covered by antibody array are depicted in green.

 

 Cancer protein profiling

Fig 7: scioDiscover has a broad coverage of diverse pathways involved in cancer. Proteins in green can be profiled with scioDiscover antibody arrays.

 

 Protein analysis in Huntington's disease

Fig. 8: scioDiscover has a good coverage of proteins involved in various diseases such as Huntington's disease. Proteins analysed in parallel by scioDiscover protein arrays are shown in green

 

Proteins involved in Tuberculosis

Fig. 9: Pathways involved in Tuberculosis are shown. Proteins covered by the scioDiscover service are shown in green.

 

scioSpot - Custom Antibody Microarrays

Antibody Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody chips as well as arrays of various affinity molecules such as Nanobodies, scFv fragments or aptamers. Benefit from excellent quality manufacturing and a wide choice of microarray support surfaces and coupling-chemistry optimised for your application.

 

Possible applications:

  • In-depth pathway profiling
  • Profiling of inflammatory markers
  • Profiling of growth factors
  • Investigate toxic and adverse drug effects in animal and cell culture models
  • Parallel Verification of biomarker candidates
  • Development of diagnostic markers
  • Highly-parallel characterisation of large numbers of antibodies
  • Identification of optimal antibodies for ELISA sandwich pairs
  • Longitudinal mouse studies
  • Correlate genomic or transcriptomic data with protein expression data

 

 Key advantages:

  • Highly-parallel protein analysis from the same sample in one experiment
  • High sensitivity (similar to ELISA or better)
  • High reproducibility (CV <13%)
  • Low sample consumption, only 10 ul plasma or serum required
  • Batch sizes from 1 up to 2.000 arrays, low batch-to-batch variation
  • Production is scalable as and when required 
  • Stringent antibody and microarray quality control

 

Reference customers:

Immunotools GmbH, Germany

 

This email address is being protected from spambots. You need JavaScript enabled to view it. to discuss your microarray project. Downlad our flyer for this service here. 

 

 

scioDiscover - Protein Biomarker Discovery

Personalised Medicine and Diagnostic Research require techniques to discover and verify novel and more accurate Biomarkers. We offer a comprehensive immuno-based Protein Biomarker Discovery Service using complex Antibody Microarrays. This platform covers key pathway proteins for various diseases as well as secreted proteins and was successfully applied in a variety of biomedical studies. The potential of our platform is underlined by the successful discovery of 4 innovative biomarker signatures.

Due to the fully immuno-based workflow a major platform change is not required for further development and therefore can decrease attrition rates of biomarker candidates. With more than 10 years of experience Sciomics is the ideal partner for Protein Biomarker Discovery and Verification. 

 

 

Biomarker Discovery for:

  • Diagnosis
  • Differential Diagnosis
  • Prognosis
  • Therapy Stratification / Drug Response Prediction
  • Companion Diagnostics

 

Features:

  • Design of appropriate study layout
  • Protein Extraction & Purification
  • Protein Quantification
  • Protein Labelling with high performance fluorescent dyes
  • Fully-automated array incubation and handling 
  • Array scanning, data acquisition and spot recognition
  • Data analysis according to your requirements
  • Individual study report

 

Key advantages:

  • Low sample consumption 
  • Investigation of human and murine samples*
  • Fast turnaround time, as low as two weeks**
  • No need for laborious depletion or fractionation of your samples
  • High sensitivity (as sensitive as an ELISA or better)
  • High reproducibility (CV < 10%)
  • Various sample types can be investigated
    • Serum / Plasma
    • Tissue
    • Cell culture
    • Cerebrospinal fluid (CSF)
  • Verification and clinical validation without major platform change
  • Parallel Verification of multiple Biomarker Candidates with Custom Antibody Arrays

 

Overview
Scio-Discover
    Array
Content
    Quality
Assurance
    Contact
us

 

 

Reference Customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

 

Case studies:

Bladder Cancer

Acute Kidney Injury

 

 

* other species on request

** depending on study design and sample numbers

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