Cytokine profiling

scioCyto is a high-content analysis service for cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell cultur supernatants can be used in this robust assay. The complete analysis services comprises all steps from protein extraction from your samples to a comprehensive study report for interpretation of the data.


  • 54 cytokines and chemokines analysed in a single assay
  • robust assay with 8 technical replicates
  • most antibodies available for follow-up studies
  • phosphorylation & ubiquitination status on request

Benefits for your research

  • comprehensive signalling information in one assay
  • low sample volumes required (10 μL plasma/serum)
  • sensitivity as ELISA-based assays
  • analysis possible from plasma/serum, tissue, cells, supernatants, CSF
  • complete analysis service (protein extraction > bioinformatic data interpretation)

scioCyto analysis process



Plasma samples 

  • Immune system activity
  • Immune response profile
  • Infection status
  • Biomarkers

Tissue samples

  • Microenvironment analysis
  • 3D cell culture models
  • Immune signalling
  • Inflammation status

Cell samples

  • Cellular signalling
  • Immune cell activation
  • Growth/stimuli profiling
  • Supernatant analysis


 Need more coverage? Have a look at our scioCD or scioDiscover service!


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scioCyto target proteins


scioCyto enables a robust profiling of 54 cytokines and chemokines in one single assay from minute sample amounts.

 sciomics scioCyto target proteins

scioUbi case study

DUB knockout cell line versus wild type

  • Direct comparison of protein level changes to ubiquitination changes
  • Easy identification of statistically significant changes
  • Immediate overview of molecular changes

sciomics scioUbi Ubiquitination And Protein Profiling case study
Overview of differentially abundant (x-axis) and ubiquitinated (y-axis) proteins from a cell culture study comparing a DUB knockout group with a wild type sample group.


Contact us   Overview scio-Ubi


Cytokine profiling: scioCyto enables a robust profiling of 54 cytokines and chemokines in one single assay from minute sample amounts.


sciomics scioCyto target proteins


Cytokine profiling - analyze 54 cytokines and chemokines in parallel

Uniprot Entry HGNC Gene names (synonym) Uniprot ID Protein names
CCL2_HUMAN CCL2 MCP1, SCYA2 P13500 C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)
CCL3_HUMAN CCL3 G0S19-1 MIP1A SCYA3 P10147 C-C motif chemokine 3 (G0/G1 switch regulatory protein 19-1) (Macrophage inflammatory protein 1-alpha) (MIP-1-alpha) (PAT 464.1) (SIS-beta) (Small-inducible cytokine A3) (Tonsillar lymphocyte LD78 alpha protein) [Cleaved into: MIP-1-alpha(4-69) (LD78-alpha(4-69))]
CCL5_HUMAN CCL5 D17S136E SCYA5 P13501 C-C motif chemokine 5 (EoCP) (Eosinophil chemotactic cytokine) (SIS-delta) (Small-inducible cytokine A5) (T cell-specific protein P228) (TCP228) (T-cell-specific protein RANTES) [Cleaved into: RANTES(3-68); RANTES(4-68)]
CCL7_HUMAN CCL7 MCP3 SCYA6 SCYA7 P80098 C-C motif chemokine 7 (Monocyte chemoattractant protein 3) (Monocyte chemotactic protein 3) (MCP-3) (NC28) (Small-inducible cytokine A7)
CCL8_HUMAN CCL8 MCP2 SCYA10 SCYA8 P80075 C-C motif chemokine 8 (HC14) (Monocyte chemoattractant protein 2) (Monocyte chemotactic protein 2) (MCP-2) (Small-inducible cytokine A8) [Cleaved into: MCP-2(6-76)]
CCL11_HUMAN CCL11 SCYA11 P51671 Eotaxin (C-C motif chemokine 11) (Eosinophil chemotactic protein) (Small-inducible cytokine A11)
CCL17_HUMAN CCL17 SCYA17 TARC Q92583 C-C motif chemokine 17 (CC chemokine TARC) (Small-inducible cytokine A17) (Thymus and activation-regulated chemokine)
CCL18_HUMAN CCL18 AMAC1 DCCK1 MIP4 PARC SCYA18 P55774 C-C motif chemokine 18 (Alternative macrophage activation-associated CC chemokine 1) (AMAC-1) (CC chemokine PARC) (Dendritic cell chemokine 1) (DC-CK1) (Macrophage inflammatory protein 4) (MIP-4) (Pulmonary and activation-regulated chemokine) (Small-inducible cytokine A18) [Cleaved into: CCL18(1-68); CCL18(3-69); CCL18(4-69)]
CD70_HUMAN CD70 CD27L CD27LG TNFSF7 P32970 CD70 antigen (CD27 ligand) (CD27-L) (Tumor necrosis factor ligand superfamily member 7) (CD antigen CD70)
CSF2_HUMAN CSF2 GMCSF P04141 Granulocyte-macrophage colony-stimulating factor (GM-CSF) (Colony-stimulating factor) (CSF) (Molgramostin) (Sargramostim)
GROA_HUMAN CXCL1 GRO GRO1 GROA MGSA SCYB1 P09341 Growth-regulated alpha protein (C-X-C motif chemokine 1) (GRO-alpha(1-73)) (Melanoma growth stimulatory activity) (MGSA) (Neutrophil-activating protein 3) (NAP-3) [Cleaved into: GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73)]
CXL10_HUMAN CXCL10 INP10 SCYB10 P02778 C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) [Cleaved into: CXCL10(1-73)]
SDF1_HUMAN CXCL12 SDF1 SDF1A SDF1B P48061 Stromal cell-derived factor 1 (SDF-1) (hSDF-1) (C-X-C motif chemokine 12) (Intercrine reduced in hepatomas) (IRH) (hIRH) (Pre-B cell growth-stimulating factor) (PBSF) [Cleaved into: SDF-1-beta(3-72); SDF-1-alpha(3-67)]
CXL13_HUMAN CXCL13 BCA1, BLC, SCYB13 O43927 C-X-C motif chemokine 13
CXL16_HUMAN CXCL16 SCYB16, SRPSOX Q9H2A7 C-X-C motif chemokine 16
FGF1_HUMAN FGF1 FGFA P05230 Fibroblast growth factor 1 (FGF-1) (Acidic fibroblast growth factor) (aFGF) (Endothelial cell growth factor) (ECGF) (Heparin-binding growth factor 1) (HBGF-1)
FGF2_HUMAN FGF2 FGFB P09038 Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
HGF_HUMAN HGF HPTA P14210 Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain]
IFNA1_HUMAN IFNA1   P01562 Interferon alpha-1/13 (IFN-alpha-1/13) (Interferon alpha-D) (LeIF D)
IFNG_HUMAN IFNG   P01579 Interferon gamma (IFN-gamma) (Immune interferon)
IL1A_HUMAN IL1A IL1F1 P01583 Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
IL1B_HUMAN IL1B IL1F2 P01584 Interleukin-1 beta (IL-1 beta) (Catabolin)
IL2_HUMAN IL2   P60568 Interleukin-2 (IL-2) (T-cell growth factor) (TCGF) (Aldesleukin)
IL4_HUMAN IL4   P05112 Interleukin-4 (IL-4) (B-cell stimulatory factor 1) (BSF-1) (Binetrakin) (Lymphocyte stimulatory factor 1) (Pitrakinra)
IL6_HUMAN IL6 IFNB2 P05231 Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2)
IL7_HUMAN IL7   P13232 Interleukin-7 (IL-7)
IL8_HUMAN CXCL8 IL8 P10145 Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I); Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b); IL-8(5-77); IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF); IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV); IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V); IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
IL10_HUMAN IL10   P22301 Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
IL12A_HUMAN IL12A NKSF1 P29459 Interleukin-12 subunit alpha (IL-12A) (Cytotoxic lymphocyte maturation factor 35 kDa subunit) (CLMF p35) (IL-12 subunit p35) (NK cell stimulatory factor chain 1) (NKSF1)
IL12B_HUMAN IL12B NKSF2 P29460 Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40) (NK cell stimulatory factor chain 2) (NKSF2)
IL13_HUMAN IL13 NC30 P35225 Interleukin-13 (IL-13)
IL15_HUMAN IL15   P40933 Interleukin-15 (IL-15)
IL16_HUMAN IL16   Q14005 Pro-interleukin-16 [Cleaved into: Interleukin-16 (IL-16) (Lymphocyte chemoattractant factor) (LCF)]
IL17_HUMAN IL17A CTLA8 IL17 Q16552 Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8)
IL18_HUMAN IL18 IGIF IL1F4 Q14116 Interleukin-18 (IL-18) (Iboctadekin) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
IL19_HUMAN IL19 ZMDA1 Q9UHD0 Interleukin-19
IL20_HUMAN IL20 ZCYTO10 Q9NYY1 Interleukin-20 (IL-20) (Cytokine Zcyto10)
IL22_HUMAN IL22 ILTIF ZCYTO18 Q9GZX6 Interleukin-22 (IL-22) (Cytokine Zcyto18) (IL-10-related T-cell-derived-inducible factor) (IL-TIF)
IL33_HUMAN IL33 C9orf26, IL1F11, NFHEV O95760 Interleukin-33
IL34_HUMAN IL34 C16orf77 Q6ZMJ4 Interleukin-34
IL36A_HUMAN IL36A FIL1E IL1E IL1F6 Q9UHA7 Interleukin-36 alpha (FIL1 epsilon) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 6) (IL-1F6)
IL36G_HUMAN IL36G IL1E IL1F9 IL1H1 IL1RP2 Q9NZH8 Interleukin-36 gamma (IL-1-related protein 2) (IL-1RP2) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 9) (IL-1F9) (Interleukin-1 homolog 1) (IL-1H1)
IL37_HUMAN IL37 FIL1Z IL1F7 IL1H4 IL1RP1 Q9NZH6 Interleukin-37 (FIL1 zeta) (IL-1X) (Interleukin-1 family member 7) (IL-1F7) (Interleukin-1 homolog 4) (IL-1H) (IL-1H4) (Interleukin-1 zeta) (IL-1 zeta) (Interleukin-1-related protein) (IL-1RP1) (Interleukin-23) (IL-37)
LIF_HUMAN LIF HILDA P15018 Leukemia inhibitory factor (LIF) (Differentiation-stimulating factor) (D factor) (Melanoma-derived LPL inhibitor) (MLPLI) (Emfilermin)
TIMP1_HUMAN TIMP1 CLGI TIMP P01033 Metalloproteinase inhibitor 1 (Erythroid-potentiating activity) (EPA) (Fibroblast collagenase inhibitor) (Collagenase inhibitor) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
TIMP3_HUMAN TIMP3   P35625 Metalloproteinase inhibitor 3 (Protein MIG-5) (Tissue inhibitor of metalloproteinases 3) (TIMP-3)
TN13B_HUMAN TNFSF13B BAFF BLYS TALL1 TNFSF20 ZTNF4 Q9Y275 Tumor necrosis factor ligand superfamily member 13B (B lymphocyte stimulator) (BLyS) (B-cell-activating factor) (BAFF) (Dendritic cell-derived TNF-like molecule) (TNF- and APOL-related leukocyte expressed ligand 1) (TALL-1) (CD antigen CD257) [Cleaved into: Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form]
TNF10_HUMAN TNFSF10 APO2L TRAIL P50591 Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)
TNF13_HUMAN TNFSF13 APRIL TALL2 ZTNF2 O75888 Tumor necrosis factor ligand superfamily member 13 (A proliferation-inducing ligand) (APRIL) (TNF- and APOL-related leukocyte expressed ligand 2) (TALL-2) (TNF-related death ligand 1) (TRDL-1) (CD antigen CD256)
TNFA_HUMAN TNF TNFA TNFSF2 P01375 Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular domain 1 (ICD1); Intracellular domain 2 (ICD2); C-domain 1; C-domain 2; Tumor necrosis factor, soluble form]
TNFB_HUMAN LTA TNFB TNFSF1 P01374 Lymphotoxin-alpha (LT-alpha) (TNF-beta) (Tumor necrosis factor ligand superfamily member 1)
TNFL4_HUMAN TNFSF4 TXGP1 P23510 Tumor necrosis factor ligand superfamily member 4 (Glycoprotein Gp34) (OX40 ligand) (OX40L) (TAX transcriptionally-activated glycoprotein 1) (CD antigen CD252)
TNFL8_HUMAN TNFSF8 CD30L, CD30LG P32971 Tumor necrosis factor ligand superfamily member 8
TSLP_HUMAN TSLP   Q969D9 Thymic stromal lymphopoietin



Contact us   Overview scio-Cyto


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