scioSpot - Custom Protein Arrays

Protein Microarrays - manufactured exactly to your requirements. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your Protein Arrays for various applications. Benefit from excellent quality manufacturing and a wide choice of microarray support surfaces and coupling-chemistry optimised for your application.

 

Possible applications:

  • Multiplexed autoimmune biomarker detection 
  • Patient stratification according to autoimmune biomarker profiles for Precision Medicine applications
  • Diagnostic tests for point-of-care IVD
  • Autoantibody Biomarker development
  • Autoimmune fingerprint profiling
  • Protein-Protein Interaction studies
  • Enzyme substrate discovery and investigation
  • Protein-DNA Interaction studies

 

Key advantages:

  • Highly-parallel analysis from the same sample in one experiment
  • Dual-colour experimental design improves robustness  
  • High sensitivity
  • High reproducibility (CV <10%)
  • Low sample consumption
  • Batch sizes from 1 up to 2.000 arrays, low batch-to-batch variation
  • Production is scalable as and when required 
  • Stringent microarray quality control

 

This email address is being protected from spambots. You need JavaScript enabled to view it. for further details and to discuss your custom protein array production.

scioSpot - Custom DNA Arrays

DNA Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your DNA arrays as well as RNA arrays. Benefit from excellent quality manufacturing and a wide choice of microarray support surfaces and coupling-chemistry optimised for your application.

 

Possible applications:

  • Diagnostic tests such as point-of-care SNP detection
  • Multiplexed pathogen detection for infectious diseases or food safety applications
  • Patient stratification according to SNP for Precision Medicine applications
  • DNA and RNA Biomarker development
  • In-depth gene expression profiling
  • Comparitive genomic studies
  • Detection of alternative splicing e.g. for oncological indications
  • Obtain and correlate genomic or transcriptomic data with protein expression data

 Key advantages:

  • Highly-parallel DNA/RNA analysis from the same sample in one experiment
  • Dual-colour experimental design improves robustness  
  • High sensitivity
  • High reproducibility (CV <13%)
  • Low sample consumption
  • Batch sizes from 1 up to 2.000 arrays, low batch-to-batch variation
  • Production is scalable as and when required 
  • Stringent microarray quality control

 

Reference customers:

Hutman Diagnostics, Switzerland

Dr. Stéphanie Renaud, University of Lausanne, Switzerland

 

This email address is being protected from spambots. You need JavaScript enabled to view it. for further details and to discuss your project. 

 

scioSpot - Custom Peptide Microarrays

Peptide Microarrays - manufactured exactly to your requirements. Using innovative laser-based technology for designing and printing custom Peptide Arrays we provide you with exactly the peptide composition you need. Fast turn-around times and cost-efficient manufacturing enables various study sizes from one microarray to hundreds.  

 

Possible applications:

  • Multiplexed autoimmune biomarker detection 
  • Autoantibody Biomarker development
  • Epitope Mapping
  • Detection of anti-pathogen antibodies
  • Detection of disease specific antibodies for e.g. HIV, Tuberculosis, CMV and Hepatitis
  • Autoimmune fingerprint profiling
  • Protein-Peptide Interaction studies

 

 Key advantages:

  • Highly-parallel analysis from the same sample in one experiment
  • Very cost-efficient even for low sample numbers
  • Production of a single slides possible  
  • Low sample consumption
  • Production is scalable as and when required 
  • Stringent microarray quality control

 

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scioDiscover - Protein Expression Analysis Platform

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1030 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples. 

 

Application Sample types - Sample requirement Advantages Service includes
       
Signalling Pathway Profiling plasma/serum - 10 ul high-content sample preparation
Immunsystem activation marker profiling tissue - 50 mg 1030 proteins analysed protein extraction
Transcription factor analysis  cells - 5 million   protein concentration measurements
Cancer pathway screening cerebrospinal fluid - 30 ul broad coverage of:  protein quality control
Cell surface marker Profiling cell culture supernatant - 10-20 ml - signalling pathway proteins  
Profiling of apoptotic processes/ apoptosis pathway analysis    - transcription factors sample labelling
  others on request - apoptosis marker sample purification
Disease mechanism profiling   - markers for oxidative stress sample incubation on arrays
Pathway activation in stem cells    - cell surface markers  
Analysis of organ damage/failure models    - cytokines/chemokines raw data acquisition
Response of cells and tissue models to oxidative stress analysis     data normalisation
Profiling proteomic changes for various cell culture conditions   minimal sample requirements data analysis
    native matrixes (non-fractionated/non-depleted) statistical analysis
Molecular initiate event analysis   sensitivity as ELISA or better comprehensive study report
Compound mode-of-action analysis      
Prediction markers for toxicological effects    fully immuno-based assay  
       
Biomarker Discovery   Reliable dual-colour study design  
Drug Target Discovery   high reproducibility, CV < 10%  
       
CD cell surface molecule expression in immune cells        

 

Target coverage - GO annotations

Biological Process   Cellular Component   Protein Classes   
cellular process (GO:0009987) 438 cell part (GO:0044464) 172 signaling molecule (PC00207) 122
metabolic process (GO:0008152) 410 organelle (GO:0043226) 97 receptor (PC00197) 113
response to stimulus (GO:0050896) 228 extracellular region (GO:0005576) 60 nucleic acid binding (PC00171) 109
biological regulation (GO:0065007) 220 membrane (GO:0016020) 59 hydrolase (PC00121) 77
developmental process (GO:0032502) 172 macromolecular complex (GO:0032991) 47 transcription factor (PC00218) 71
immune system process (GO:0002376) 145 extracellular matrix (GO:0031012) 28 enzyme modulator (PC00095) 67
localization (GO:0051179) 116 cell junction (GO:0030054) 3 cell adhesion molecule (PC00069) 62
multicellular organismal process (GO:0032501) 72 synapse (GO:0045202) 2 defense/immunity protein (PC00090) 52
apoptotic process (GO:0006915) 67     cytoskeletal protein (PC00085) 40
cellular component organization or biogenesis (GO:0071840) 65     oxidoreductase (PC00176) 31
biological adhesion (GO:0022610) 60     kinase (PC00137) 31
reproduction (GO:0000003) 26     transporter (PC00227) 27
locomotion (GO:0040011) 4     transfer/carrier protein (PC00219) 25
        calcium-binding protein (PC00060) 20
        phosphatase (PC00181) 20
        structural protein (PC00211) 18
        extracellular matrix protein (PC00102) 17
        cell junction protein (PC00070) 17
        membrane traffic protein (PC00150) 14

PLEASE NOTE: GO annotation terms may overlap 

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Reference customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

scioUbi

Protein & ubiquitination profiling

scioUbi is a high-content protein expression and ubiquitination level analysis.
With scioUbi you can analyse up to 1,000 proteins in a single assay.

 

Features

  • Ubiquitination status for more than 1,000 proteins in parallel
    in a single assay
  • 4 technical replicates per sample
  • Comprehensive data analysis and presentation including
    an individualised report
  • Customisation of ubiquitin detection on request

Benefits for your research

  • Two in one: High-content protein expression and ubiquitination level analysis in a single assay
  • Proteins selected for biomedical studies: Central regulators (e.g. mTor, p53), transcription factors, receptors
  • Minimal sample (e.g. tissue or cell culture samples) amounts needed for complete analysis
sciomics scioUbi Ubiquitination And Protein Profiling Biological processes selection of proteins
Biological processes
  sciomics scioUbi Ubiquitination And Protein Profiling Cell Cycle proteins coveredCell cycle: proteins in green are covered by the analysis

 

Applications

Biological effects of

  • protein degradation in health and disease
  • protein activity changes
  • protein interaction alterations
  • drug response (e.g. proteasome enhancers)

Others

  • Interplay between ubiquitination and phosphorylation (see scioPhospho)
  • Neurodegenerative diseases
  • Progression of ageing
  • Cell cycle regulation
  • Proteasome activity
  • Transcriptional regulation
  • p53 pathway regulation
  • Alzheimer pathway regulation

 

Contact us

 

Subcategories