scioSpot - Custom Protein Arrays

Protein Microarrays - manufactured exactly to your requirements. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your Protein Arrays for various applications. Benefit from excellent quality manufacturing and a wide choice of microarray support surfaces and coupling-chemistry optimised for your application.

 

Possible applications:

  • Multiplexed autoimmune biomarker detection 
  • Patient stratification according to autoimmune biomarker profiles for Precision Medicine applications
  • Diagnostic tests for point-of-care IVD
  • Autoantibody Biomarker development
  • Autoimmune fingerprint profiling
  • Protein-Protein Interaction studies
  • Enzyme substrate discovery and investigation
  • Protein-DNA Interaction studies

 

Key advantages:

  • Highly-parallel analysis from the same sample in one experiment
  • Dual-colour experimental design improves robustness  
  • High sensitivity
  • High reproducibility (CV <10%)
  • Low sample consumption
  • Batch sizes from 1 up to 2.000 arrays, low batch-to-batch variation
  • Production is scalable as and when required 
  • Stringent microarray quality control

 

This email address is being protected from spambots. You need JavaScript enabled to view it. for further details and to discuss your custom protein array production.

scioSpot - Custom DNA Arrays

DNA Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your DNA arrays as well as RNA arrays. Benefit from excellent quality manufacturing and a wide choice of microarray support surfaces and coupling-chemistry optimised for your application.

 

Possible applications:

  • Diagnostic tests such as point-of-care SNP detection
  • Multiplexed pathogen detection for infectious diseases or food safety applications
  • Patient stratification according to SNP for Precision Medicine applications
  • DNA and RNA Biomarker development
  • In-depth gene expression profiling
  • Comparitive genomic studies
  • Detection of alternative splicing e.g. for oncological indications
  • Obtain and correlate genomic or transcriptomic data with protein expression data

 Key advantages:

  • Highly-parallel DNA/RNA analysis from the same sample in one experiment
  • Dual-colour experimental design improves robustness  
  • High sensitivity
  • High reproducibility (CV <13%)
  • Low sample consumption
  • Batch sizes from 1 up to 2.000 arrays, low batch-to-batch variation
  • Production is scalable as and when required 
  • Stringent microarray quality control

 

Reference customers:

Hutman Diagnostics, Switzerland

Dr. Stéphanie Renaud, University of Lausanne, Switzerland

 

This email address is being protected from spambots. You need JavaScript enabled to view it. for further details and to discuss your project. 

 

scioSpot - Custom Peptide Microarrays

Peptide Microarrays - manufactured exactly to your requirements. Using innovative laser-based technology for designing and printing custom Peptide Arrays we provide you with exactly the peptide composition you need. Fast turn-around times and cost-efficient manufacturing enables various study sizes from one microarray to hundreds.  

 

Possible applications:

  • Multiplexed autoimmune biomarker detection 
  • Autoantibody Biomarker development
  • Epitope Mapping
  • Detection of anti-pathogen antibodies
  • Detection of disease specific antibodies for e.g. HIV, Tuberculosis, CMV and Hepatitis
  • Autoimmune fingerprint profiling
  • Protein-Peptide Interaction studies

 

 Key advantages:

  • Highly-parallel analysis from the same sample in one experiment
  • Very cost-efficient even for low sample numbers
  • Production of a single slides possible  
  • Low sample consumption
  • Production is scalable as and when required 
  • Stringent microarray quality control

 

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scioCD - Cell surface marker and Cytokine profiling_v2

 

Characterise a certain cell type ?

 

Cell composition in a certain tissue sample ?

 

In-depth profiling of CD-marker expression ?

 

Increase your throughput at high sensitivity levels ?

You aim at high reproducibility ?

 

 

Our scioCD profiling service covers 206 different proteins by monoclonal antibodies:

All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of tissues or the differentiation status of cultured cells in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently, providing insights in the blood cell composition.

Combining analysis of signalling and cellular receptor proteins provides in-depth insights into signal transduction processes
Proteins profiled in parallel by scioCD antibody microarray and their interactions

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

 

 

Target
proteins
      Analysis
Service
      Sample
Types
      Product
brochure
      Contact
us

 

 

 

 

Applications

  • profiling of cell differentiation status
  • stem cell differentiation 
  • investigate soluble CD-molecules for e.g. allograft rejection
  • inflammatory response profiling
  • immuno-oncology profiling of check-point inhibitor targets
  • characterisation of tissue-isolated primary cells
  • immune-cell surface marker profiling on
    • various B-cells 
    • stimulated T-cells
    • patient derived T-cells
    • patient derived dendritic cells
  • oncology profiling for new biomarkers e.g. Leukemia
  • tumor micro-environment profiling
  • profile response to stimuli of cell lines
  • evaluate protein expression changes in silencing/overexpression  experiments
 

Advantages

  • high content - 206 proteins are analysed with monoclonal antibodies
  • broad coverage and parallel analysis of
    • 129 CD molecules 
    • 54 cytokines and chemokines
    • 23 other relevant molecules (HLAs, p53)
  • minimal sample requirements
  • native matrixes ( non-fractionated / non-depleted )
  • sensitivity as ELISA or better
  • fully immuno-based assay
  • reliable dual-colour study design
  • high reproducibility, CV < 10%
  • most proteins are covered redundantly by two or more antibodies
  • each antibody is presented in four replicates
  • antibodies are available for follow-up studies


 

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request

  

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

scioDiscover - Protein Expression Analysis Platform

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1030 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples. 

 

ApplicationSample types - Sample requirementAdvantagesService includes
    
Signalling Pathway Profilingplasma/serum - 10 ulhigh-contentsample preparation
Immunsystem activation marker profilingtissue - 50 mg1030 proteins analysedprotein extraction
Transcription factor analysis cells - 5 million protein concentration measurements
Cancer pathway screeningcerebrospinal fluid - 30 ulbroad coverage of: protein quality control
Cell surface marker Profilingcell culture supernatant - 10-20 ml- signalling pathway proteins 
Profiling of apoptotic processes/ apoptosis pathway analysis  - transcription factorssample labelling
 others on request- apoptosis markersample purification
Disease mechanism profiling - markers for oxidative stresssample incubation on arrays
Pathway activation in stem cells  - cell surface markers 
Analysis of organ damage/failure models  - cytokines/chemokinesraw data acquisition
Response of cells and tissue models to oxidative stress analysis  data normalisation
Profiling proteomic changes for various cell culture conditions minimal sample requirementsdata analysis
  native matrixes (non-fractionated/non-depleted)statistical analysis
Molecular initiate event analysis sensitivity as ELISA or bettercomprehensive study report
Compound mode-of-action analysis   
Prediction markers for toxicological effects  fully immuno-based assay 
    
Biomarker Discovery Reliable dual-colour study design 
Drug Target Discovery high reproducibility, CV < 10% 
    
CD cell surface molecule expression in immune cells     

 

Target coverage - GO annotations

Biological Process Cellular Component Protein Classes  
cellular process (GO:0009987)438cell part (GO:0044464)172signaling molecule (PC00207)122
metabolic process (GO:0008152)410organelle (GO:0043226)97receptor (PC00197)113
response to stimulus (GO:0050896)228extracellular region (GO:0005576)60nucleic acid binding (PC00171)109
biological regulation (GO:0065007)220membrane (GO:0016020)59hydrolase (PC00121)77
developmental process (GO:0032502)172macromolecular complex (GO:0032991)47transcription factor (PC00218)71
immune system process (GO:0002376)145extracellular matrix (GO:0031012)28enzyme modulator (PC00095)67
localization (GO:0051179)116cell junction (GO:0030054)3cell adhesion molecule (PC00069)62
multicellular organismal process (GO:0032501)72synapse (GO:0045202)2defense/immunity protein (PC00090)52
apoptotic process (GO:0006915)67  cytoskeletal protein (PC00085)40
cellular component organization or biogenesis (GO:0071840)65  oxidoreductase (PC00176)31
biological adhesion (GO:0022610)60  kinase (PC00137)31
reproduction (GO:0000003)26  transporter (PC00227)27
locomotion (GO:0040011)4  transfer/carrier protein (PC00219)25
    calcium-binding protein (PC00060)20
    phosphatase (PC00181)20
    structural protein (PC00211)18
    extracellular matrix protein (PC00102)17
    cell junction protein (PC00070)17
    membrane traffic protein (PC00150)14

PLEASE NOTE: GO annotation terms may overlap 

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Reference customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

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