scioDiscover - Protein Biomarker Discovery

Personalised Medicine and Diagnostic Research require techniques to discover and verify novel and more accurate Biomarkers. We offer a comprehensive immuno-based Protein Biomarker Discovery Service using complex Antibody Microarrays. This platform covers key pathway proteins for various diseases as well as secreted proteins and was successfully applied in a variety of biomedical studies. The potential of our platform is underlined by the successful discovery of 4 innovative biomarker signatures.

Due to the fully immuno-based workflow a major platform change is not required for further development and therefore can decrease attrition rates of biomarker candidates. With more than 10 years of experience Sciomics is the ideal partner for Protein Biomarker Discovery and Verification. 

 

 

Biomarker Discovery for:

  • Diagnosis
  • Differential Diagnosis
  • Prognosis
  • Therapy Stratification / Drug Response Prediction
  • Companion Diagnostics

 

Features:

  • Design of appropriate study layout
  • Protein Extraction & Purification
  • Protein Quantification
  • Protein Labelling with high performance fluorescent dyes
  • Fully-automated array incubation and handling 
  • Array scanning, data acquisition and spot recognition
  • Data analysis according to your requirements
  • Individual study report

 

Key advantages:

  • Low sample consumption 
  • Investigation of human and murine samples*
  • Fast turnaround time, as low as two weeks**
  • No need for laborious depletion or fractionation of your samples
  • High sensitivity (as sensitive as an ELISA or better)
  • High reproducibility (CV < 10%)
  • Various sample types can be investigated
    • Serum / Plasma
    • Tissue
    • Cell culture
    • Cerebrospinal fluid (CSF)
  • Verification and clinical validation without major platform change
  • Parallel Verification of multiple Biomarker Candidates with Custom Antibody Arrays

 

Overview
Scio-Discover     Array
Content     Quality
Assurance     Contact
us

 

 

Reference Customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

  

Case studies:

Bladder Cancer

Acute Kidney Injury

 

 

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

** depending on study design and sample numbers

scioDiscover - Protein Expression Analysis Platform

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1,300 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples. 

 

Application Sample types - Sample requirement Advantages Service includes
       
Signalling Pathway Profiling plasma/serum - 10 ul high-content sample preparation
Immunsystem activation marker profiling tissue - 50 mg 1030 proteins analysed protein extraction
Transcription factor analysis  cells - 5 million   protein concentration measurements
Cancer pathway screening cerebrospinal fluid - 30 ul broad coverage of:  protein quality control
Cell surface marker Profiling cell culture supernatant - 10-20 ml - signalling pathway proteins  
Profiling of apoptotic processes/ apoptosis pathway analysis    - transcription factors sample labelling
  others on request - apoptosis marker sample purification
Disease mechanism profiling   - markers for oxidative stress sample incubation on arrays
Pathway activation in stem cells    - cell surface markers  
Analysis of organ damage/failure models    - cytokines/chemokines raw data acquisition
Response of cells and tissue models to oxidative stress analysis     data normalisation
Profiling proteomic changes for various cell culture conditions   minimal sample requirements data analysis
    native matrixes (non-fractionated/non-depleted) statistical analysis
Molecular initiate event analysis   sensitivity as ELISA or better comprehensive study report
Compound mode-of-action analysis      
Prediction markers for toxicological effects    fully immuno-based assay  
       
Biomarker Discovery   Reliable dual-colour study design  
Drug Target Discovery   high reproducibility, CV < 10%  
       
CD cell surface molecule expression in immune cells        

 

Target coverage - GO annotations

Biological Process Cellular Component Protein Classes 
cellular process (GO:0009987) 438 cell part (GO:0044464) 172 signaling molecule (PC00207) 122
metabolic process (GO:0008152) 410 organelle (GO:0043226) 97 receptor (PC00197) 113
response to stimulus (GO:0050896) 228 extracellular region (GO:0005576) 60 nucleic acid binding (PC00171) 109
biological regulation (GO:0065007) 220 membrane (GO:0016020) 59 hydrolase (PC00121) 77
developmental process (GO:0032502) 172 macromolecular complex (GO:0032991) 47 transcription factor (PC00218) 71
immune system process (GO:0002376) 145 extracellular matrix (GO:0031012) 28 enzyme modulator (PC00095) 67
localization (GO:0051179) 116 cell junction (GO:0030054) 3 cell adhesion molecule (PC00069) 62
multicellular organismal process (GO:0032501) 72 synapse (GO:0045202) 2 defense/immunity protein (PC00090) 52
apoptotic process (GO:0006915) 67     cytoskeletal protein (PC00085) 40
cellular component organization or biogenesis (GO:0071840) 65     oxidoreductase (PC00176) 31
biological adhesion (GO:0022610) 60     kinase (PC00137) 31
reproduction (GO:0000003) 26     transporter (PC00227) 27
locomotion (GO:0040011) 4     transfer/carrier protein (PC00219) 25
        calcium-binding protein (PC00060) 20
        phosphatase (PC00181) 20
        structural protein (PC00211) 18
        extracellular matrix protein (PC00102) 17
        cell junction protein (PC00070) 17
        membrane traffic protein (PC00150) 14

PLEASE NOTE: GO annotation terms may overlap 

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Reference customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

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