scioDiscover - Protein Expression Analysis

Protein expression profiling with protein arrays

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 900 proteins are analysed in parallel covered by 1130 antibodies. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples.

 

Features

  • Major pathways, secreted proteins, cell surface and inflammatory markers are covered
  • > 900 analytes
  • > 1,100 antibodies
  • Broad spectrum of indications covered such as oncology and organ failure
  • Robust dual-colour study design
  • Including full data analysis and study report
  • Studies possible in human, murine and rat samples 

 

 Array
 content
    Protein
 profiling
    Biomarker
 Discovery
    Target
 Discovery
    Contact
 us

 

 

Applications

  • Protein expression level analysis
  • Signalling Pathway Profiling
  • Immunsystem activation marker profiling
  • Transcription factor analysis 
  • Cancer pathway screening
  • Cell surface marker profiling
  • Profiling of apoptotic processes/ apoptosis pathway analysis 

 

  • Disease mechanism profiling
  • Pathway activation in stem cells 
  • Analysis of organ damage/failure models 
  • Response of cells and tissue models to oxidative stress analysis
  • Profiling proteomic changes for various cell culture conditions

 

  • Molecular initiate event analysis
  • Compound mode-of-action analysis
  • Prediction markers for toxicological effects 

 

 

  • CD cell surface molecule expression in immune cells  

 

 

 

 

Advantages

  • high-content: 900 proteins analysed with 1130 antibodies
  • broad coverage of:
    • signalling pathway proteins
    • transcription factors
    • apoptosis marker
    • markers for oxidative stress
    • cell surface markers
    • cytokines & chemokines

 

  • minimal sample requirements
  • native matrixes (non-fractionated / non-depleted)
  • sensitivity as ELISA or better

 

  • fully immuno-based assay

 

  • reliable dual-colour study design
  • technical replication ( 4 replicates for each antibody )
  • high reproducibility ( CV < 10% )

 

  • experienced partners with more than ten years of experience in conducting biomedical antibody array studies

 

 

Save time, costs and valuable sample

 

 

 

Reference Customers:

  • Prof. Dr. Faikah Güler, Molecular mechanisms of transplant dysfunction, Medical School Hanover, Hanover, Germany
  • Julian Kamhieh-Milz, Research Center ImmunoSciences, Charité, Berlin, Germany
  • Prof. Jose L. Peiró, Cincinnati Fetal Center Director, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, USA
 

Case studies:

Bladder Cancer

Acute Kidney Injury


 

Which sample types can be analysed ?

With the scioDiscover service a variety of samples can be analysed:

  • plasma / serum
  • fresh frozen tissue samples
  • cellular content
  • cell culture supernatant
  • cerebrospinal fluid
  • additional sample types on request

  

Sample-to-result serviceAntibody microarray workflow

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

  • definition of an appropriate study design
  • sample preparation
  • protein extraction
  • protein concentration measurements
  • protein quality control
  • sample labelling
  • sample purification
  • incubation of the samples on antibody microarrays
  • microarray scanning
  • raw data acquisition
  • data normalisation
  • data analysis including cluster analysis
  • statistical testing for differentially abundant proteins
  • comprehensive study report

 

 

Do you like to subscribe our newsletter ?      Yes   No

Products & Services

scioDiscover - Protein Expression Level Profiling

scioDiscover - Protein Expression Level Profiling

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower…
Read more
scioDiscover - Protein Expression Analysis

scioDiscover - Protein Expression Analysis

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker…
Read more
scioDiscover - Protein Biomarker Discovery

scioDiscover - Protein Biomarker Discovery

Personalised Medicine and Diagnostic Research require techniques to discover and verify novel and more accurate Biomarkers. We offer a comprehensive immuno-based Protein Biomarker Discovery Service using complex Antibody Microarrays. This…
scioPhospho: protein + phosphorylation profiling

scioPhospho: protein + phosphorylation profiling

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway…
scioExtract - Protein Extraction Buffers

scioExtract - Protein Extraction Buffers

The Scio-Extract Protein Extraction Buffers are optimised for superior protein isolation performance from all cell compartments and of membrane proteins. Their non-denaturing properties enable the extraction of active proteins under…
scioCD - CD-marker antibody array

scioCD - CD-marker antibody array

Characterise a certain cell type ? Cell composition in a certain tissue sample ? In-depth profiling of CD-marker expression ? Increase your throughput at high sensitivity levels ? You aim…
scioSpot - Microarray Production

scioSpot - Microarray Production

Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody-, protein-, peptide- or DNA/RNA chips. Benefit from…
scioSpot - Custom Antibody Microarrays

scioSpot - Custom Antibody Microarrays

Antibody Microarrays - tailor-made for you. With more than 10 years of experience, Sciomics is the ideal partner for designing and printing your antibody chips as well as arrays of…
scioSpec - Antibody Specificity Assessment

scioSpec - Antibody Specificity Assessment

Be sure that your antibody is fit for your application. Profile your therapeutic or diagnostic antibody against more than 9000 human proteins. Have a comprehensive cross-reactivity and off-target reactivity profile…
scioAuto - Autoantibody Discovery

scioAuto - Autoantibody Discovery

Profile your patient samples against more than 9000 human proteins. Let us help you with our comprehensive fast and cost-effective autoantibody biomarker discovery study service. Our comprehensive autoantibody discovery service…

News

New article | Expansion of a BDCA1+CD14+ Myeloid Cell Population in Melanoma Patients May Attenuate the Efficacy of Dendritic Cell Vaccines.

| July 2016 | One of our customer projects was published in Cancer Research. In this study Bakdash et al. analysed the expansion of a BDCA1+CD14+ myeloid cell population in the context of immune therapy of melanoma. Within this study a protein profiling of cellular patient samples was performed with scioCD antibody arrays.

Interview | Advanced Biomarkers for Precision Medicine

New article | Analysis of the blood protein profile in B-cell lymphoma patients

| 12.12.2013 | There is a new article in the current issue of Proteomics Clinical Applications on an analysis of the blood protein profile of different B-cell lymphoma using complex antibody microarrays. 200 blood samples from patients with Diffuse Large B-cell Lymphoma (DLBCL), Follicular Lymphoma (FL), Chronic Lymphocytic Leukemia (CLL) as well as healthy controls were analysed and compared with each other. The study identified proteins with discriminating power among the different lymphoma types, as well as subgroups within each lymphoma entity.

New article | Prediction of recurrence in bladder cancer

| 08.05.2014 | In a study published in Proteomics we identified proteins with differential abundance in bladder tumours with and without a local recurrence using antibody array technology. Next to interesting differences of the two tumour subtypes from a scientific point of view, we found a protein biomarker signature with high potential for a prognosis. This could enabling urologists in future to adjust the therapy and surveillance scheme accordingly. Further validation studies are ongoing. →Case study report

Tweets

  • 8 hours ago

    @FedExHelp thank you for your prompt replies and taking care, while we are waiting for any reply from your Germany team.

  • 9 hours ago

    @FedExHelp yes, the form was completed, refill is urgently needed. No response to the return of the form for some hours now again.

  • 10 hours ago

    @FedExHelp experiencing problems with almost all packages sent to us by our partners / customers if sent via FedEx - no problems with TNT

  • 10 hours ago

    @FedExHelp We received the dry ice form 27h after initial request and after our post here, probably to late for a refill on Fri afternoon.

  • 12 hours ago

    @FedExHelp +49 6221 4294830