scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation.

Features

  • 1030 highly relevant proteins are profiled in a single assay
  • Phosphorylation status combined with protein expression levels
  • Tyrosine, serine and threonine phosphorylation
  • Analysis of individual phosphorylation types available
  • "Sample-to-result" service
  • Comprehenisive data analysis and presentation
  • Individualised study report
Protein abundance levels and phosphorylation status for one individual protein out of 900 proteins tested

Advantages

  • High-content screening of proteins and phosphorylation is time and ressource efficient
  • Direct comparability through protein expression and phosphorylation level analysis in a single assay
  • Complete analysis from minimal sample volume
  • Coverage of important
    • Signalling pathways
    • Transcription factors
    • Receptor molecules
    • Kinases
  • Individual data analysis options available
    • Protein interaction network analysis
    • Biological and pathway classification 
    • Lists with differential proteins and phosphorylation levels
  • Variety of sample formats possible without the potential bias of a sample fractionation or a sample depletion
  • Results presented in a publication-ready format

Applications

  • T cell activation
  • Tissue treatment response
  • Cell cycle regulation
  • Oxidative stress response
  • Cell adhesion and motility
  • Phosphorylation profiling of various signalling pathways:
    • FAK phosphorylation
    • PI3K-AKT pathway
    • p53 pathway regulation
    • Alzheimer pathway regulation

 

Case study   Target proteins   Contact us

 

Case study

Eight treated cell line samples were compared with 8 control samples. Several proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). While the left plot provides an overview on expression / phosphorylation differences for all tested proteins in the two sample groups, individual levels are depicted in the plots on the right. The sample status is indicated as C = control or T = treated.

 

Reference customers

Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany

Dr. Caroline Pabst. University Hospital Halle, Germany

 

More information

Download our scioPhospho or scioDiscover service brochure.

Information on protein and pathway content / coverage.

Get in touch to answer any remaining questions.

Ask for a quotation to get your study started.

 

Which sample types can be analysed?

With the scioPhospho service a variety of samples can be analysed:

 

Sample-to-result service

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes: