Characterisation of antibodies

Efficiently characterize the binding behaviour of your antibodies with antibody microarraysDo you have a set of antibodies against a number of target proteins ?

Antibody microarrays facilitate a fast and cost-effective method for characterising these antibodies in a parallel manner. With antibody microarrays you can:

  • analyse the binding properties of your target protein
  • analyse cross-binding profiles to other proteins
  • identify optimal pairs for a sandwich assays

In addition, we can help you to assess the specificity of your antibody or to characterize the exact epitope. Please contact us for more informations.

Applications for antibody microarrays

Antibody microarrays are a versatile tool.

Antibody microarrays are an efficient and cost-effective tool for the analysis of multiple proteins in parallel. Some advantages of an analysis based on antibody microarray are:

  • Lower antibody consumption as compared to standard assays such as ELISA
  • The highly parallel approach increases the probability to identify a protein biomarker candidate in a biomarker screening or verification study.
  • The parallel analysis of multiple target proteins facilitates a direct comparison of protein abundance in the very same experiment and set of samples.
  • Only minimal amount of sample such as 20 µL of plasma is needed to analyse hundreds of proteins. Therefore, multiplex analysis is feasible for low abundant samples.
  • A variety of sample types such as plasma, serum, tissue or samples from cell culture experiments can be analysed with the very same platform
  • A sample fractionation or a depletion of high abundant proteins such as albumin is not necessary. Thereby, the sample preparation is simplified and a potential bias can be prevented.
  • Where necessary, alternative immunological assays can be built comparably easy based on the very same antibodies.

Most of time antibody microarrays are used in biomedical studies. However, there are multiple other possibilities such as analysis and surveillance of food or the analysis of environmental samples. Contact us to learn about the advantages of an antibody microarray based analysis in your application.

Target identification

Identify the target(s) of your compound and pathways affected with antibody microarray technology

You identified a compound with the desired effect ?

You are looking for its target(s) ?

Are additional pathways affected ?


Antibody microarrays are an efficient tool to screen for targets and pathways affected by a compound or treatment. You will get the whole picture of protein expression for one or multiple pathways.

Sequencing data and protein expression

Antibody microarrays enable you to correlate your next generation sequencing data with protein expression levels.hits on 

You have a wealth of results from next generation sequencing experiments. Now you would like to know whether the alteration on DNA level has an impact on the phenotype or the level of protein expression, protein interaction partners and downstream elements? Does the mutation have therapeutic potential?

With antibody microarrays, you can investigate hundreds of your hits and their interaction partners in parallel with a minimal amount of sample.

Contact us, to connect your sequencing data with knowledge on protein expression.

Biomarker Studies

Use the approriate tool for your biomarker study. With a highly parallel testing you increase the probability to identify a suitable biomarker candidate.

Are you interested in diagnostic, prognostic or predictive protein biomarkers ?

Are you challenged to select appropriate candidates from a pilot study, from experiments using mass spectrometry or from literature searches ?

Are your samples extremely valuable and you would like to minimize the amount needed for a screening or verification study ?


Antibody microarrays offer a robust technology for biomarker screening or a verification of multiple biomarker candidates in parallel. Only minute amounts of sample, as little as 15-20µL plasma are needed and the sensitivity of this technology is as high as in ELISA.

With antibody arrays you consume less of your valuable samples and increase the probability of identifying a suitable biomarker candidate by using parallel analysis.