Scio-Extract - Tissue

Different formulations

Price list and ordering

One sample free per lab. Shipping costs may apply.

Please contact us to answer any remaining questions.

Overview Scio-Extract       Scio-Extract - Cell

DUB knockout cell line versus wild type

• Direct comparison of protein level changes to ubiquitination changes
• Easy identification of statistically significant changes
• Immediate overview of molecular changes

Overview of differentially abundant (x-axis) and ubiquitinated (y-axis) proteins from a cell culture study comparing a DUB knockout group with a wild type sample group.

Contact us   Overview scio-Ubi

Our scioDiscover service enables high-content, immuno-based protein profiling of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1,300 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples.

Features

• Major pathways, secreted proteins, cell surface and inflammatory markers are covered
• 1,300 proteins are profiled
• Broad spectrum of indications covered such as oncology and organ failure
• Robust dual-colour study design
• Including full data analysis and study report
• Studies possible in human, murine and rat samples*

 Array
 content     Protein
 profiling     Biomarker
 Discovery      Target
 Discovery      Contact
 us

Which sample types can be analysed ?

With the scioDiscover service a variety of samples can be analysed:

• plasma / serum
• fresh frozen tissue samples
• cellular content
• cell culture supernatant
• cerebrospinal fluid
• additional sample types on request
• studies possible in samples from human, mouse or rat*

_{* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].}

Sample-to-result service

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

• definition of an appropriate study design
• sample preparation
• protein extraction
• protein concentration measurements
• protein quality control
• sample labelling
• sample purification
• incubation of the samples on antibody microarrays
• microarray scanning
• raw data acquisition
• data normalisation
• data analysis including cluster analysis
• statistical testing for differentially abundant proteins
• comprehensive study report

The Scio-Extract Protein Extraction Buffers are optimised for superior protein isolation performance from all cell compartments and of membrane proteins. Their non-denaturing properties enable the extraction of active proteins under native conditions. The unique composition ensures a high overall protein yield. The Scio-Extract buffers are available for protein extraction from tissue or cell culture samples and are formulated in different ways to suit different experimental requirements. 

Major Applications

• Proteomics studies

• SDS-Page/2D-Page analysis

• Western Blotting

• Microarray analysis and labelling with fluorescent dyes

• Analysis of protein-protein interactions

• Analysis of membrane proteins

 Special / Niche Applications

• Protein Extraction from Drosophila Melanogaster

• Protein Isolation from fracture hematoma

Main Advantages - Scio-Extract Buffers

• Optimised isolation of proteins in an easy and convenient fashion

• High yield of membrane proteins

• High extraction yields from all cell compartments

• High overall protein extraction yield

• Successfully used in many biomedical studies

• Non-denaturing properties are ideal for a wide range of applications

• Fully compatible with subsequent fluorescent dye labelling

• Fully compatible with all Sciomics microarray products
  

Product lines

The extraction buffers are available in different optimised compositions for extraction from different sample types

Tissue

Optimised composition for high-yield extraction from tissue samples

Cells

Optimised composition for high-yield extraction from cultured or isolated cells

and in different formats:

Basic

Optimised composition for high-yield extraction

Advanced

Protease Inhibitor cocktail in addition to Basic composition

Pro

Protease and Phosphatase Inhibitor cocktail in addition to Basic composition

Prices and Ordering

Scio-Extract - Tissue       Scio-Extract - Cells

 Please feel free to download our flyer for these buffers and please contact us to answer any remaining questions.

overview scio-phospho   Contact us

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1,300 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples. 

Target coverage - GO annotations

PLEASE NOTE: GO annotation terms may overlap 

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Reference customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

Case study - Bladder Cancer

Case study - Acute Kidney Injury

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower cost through a highly-parallel protein expression level analysis using our Scio-Discover Antibody Microarray Platform. Furthermore, you may link to and verify your Genomics and Transcriptomics data with protein expression levels.

Applications:

• Pre-clinical Toxicology Studies
  • rodents such as mice* and rats*
  • cell culture models
  • tissue culture models
• Drug target identification and verification
• Mechanistic studies for various indications
• Profile cytokine and inflammatory status
• Protein Biomarker identification and verification

Key advantages:

• High multiplexity (1,300 proteins in parallel)
• Low sample consumption
• Investigation of human and murine samples*
• Fast turnaround time, as low as two weeks**
• No need for laborious depletion or fractionation of your samples
• High sensitivity (as sensitive as an ELISA or better)
• High reproducibility (CV < 10%)
• Various sample types can be investigated
  • Serum / Plasma
  • Tissue
  • Cell culture
  • Cerebrospinal fluid (CSF)

Overview
Scio-Discover     Pathway
Coverage     Biomarker
Discovery     Contact
us

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

.** depending on study design and sample numbers

High quality standards are essential for robust and reliable Biomarker Discovery and Verification as well as for the discovery of new Drug Targets. During the last 10 years a stringent quality assurance workflow has been established for our Antibody Array Platform in order to conduct immuno-based biomedical discovery studies with excellent standards in terms of reproducibility and platform stability. The automated assay workflow further improves the performance of our service. 

Biomarker Discovery Phase - scioDiscover Platform

• Stringent antigen design for maximised specificity of the resulting antibodies
• Antibodies are antigen affinity-purified
• Targets identified through transcriptional studies and expert input
• Stringent QC management
• Features:  
  • cross-species activity (human and mouse for high versatility)
  • detection of several protein isoforms
  • cost-efficiency through high content

Verification Phase - Custom Array Platform

• Stringent antigen design for maximised specificity of the resulting antibodies
• Antibodies are antigen affinity-purified
• In-house QC on Western Blot (different cell lines or tissues) and optional ELISA-based tests
• In-house QC on large-scale protein arrays (> 9.000 human proteins)
• In-depth validation of array performance
• Definition of biomarker signature with highest accuracy
• Selection of appropriate binders / sandwich pairs
• Features:  
  • specifically designed antibodies for further use in diagnostic assays
  • fully renewable antibody formats
  • in-depth quality controlled (WB, ELISA)
  • comprehensive cross-reactivity profile (large-scale protein arrays)

Orthogonal Biomarker Validation

Verified Candidates can be further validated by orthogonal methods according to customer’s requirements

• Western Blotting ( WB )
• ELISA
• Immuno-Precipitation ( IP )
• Mass spectrometry ( MS )
• Biacore

Overview
Scio-Discover     Biomarker
Discovery     Custom Microarrays
for Biomarker Verification     Contact
us

You identified a compound with the desired effect ?

You are looking for its target(s) ?

Which additional pathways are affected ?

Which proteins are altered in a diseased tissue ?

Get the whole picture of protein expression in multiple pathways !

Profound knowledge about protein expression levels is of utmost importance for the discovery and evaluation of new drug targets. Our Scio-Discover Service is fully immuno-based and covers proteins involved in major disease and signalling pathways as well as secreted proteins. For the highly-parallel protein analysis only minimal amounts of sample are required. No sample fractionation or depletion is needed. Our platform is able to process most biological samples without the need for laborious sample preparation, saving time and resources.

Applications:

• Drug Target Identification and verification studies
• Mechanistic studies for various indications
• Correlate Next-Gen-Sequencing Data to protein expression levels
• Profile cytokine and inflammatory status of your samples

Key advantages:

• Low sample consumption 
• Investigation of human, murine and rat samples*
• Fast turnaround time, as low as two weeks**
• No need for laborious depletion or fractionation of your samples
• High sensitivity (as sensitive as an ELISA or better)
• High reproducibility (CV < 10%)
• Various sample types can be investigated
  • Serum / Plasma
  • Tissue
  • Cell culture
  • Cerebrospinal fluid (CSF)

Service Features:

• Study Layout
• Protein Extraction and Purification
• Protein Quantification
• Protein labelling with high-performance fluorescent dyes
• Automated array incubation and handling 
• Array scanning and data acquisition
• Data analysis according to your requirements
• Individual study report

Overview
Scio-Discover     Array
Content     Biomarker
Discovery     Contact
us

_{* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].}

_{** depending on study design and sample numbers}